Stains and staining procedures in Pathology pptx

Stains used in Pathology

1. PRACTICAL-STAINING DR.K.L.MISHRA(ASSISTANT PROFESSOR)

2. acidic and basic components of Romanowsky stain Acidic stain-  Eosin Y – stains basic components like cytoplasmic  granules  and RBCs  in a pink colour Basic stain –  Methylene blue – stains acidic components like  basophilic granules in the cytoplasm, nuclei of  all leukocytes  in a blue violet colour

3. Field’s stain Preparation of stain Stain A  (Polychrome methylene blue) Methylene blue – 0.26g Azure B- 0.1g Disodium hydrogen phosphate – 2.5g Potassium dihydrogen phosphate – 1.25g Water – 100 ml Stain B ( Eosin) Eosin (Water soluble yellow Eosin) – 0.26g Disodium hydrogen phosphate – 2.5g Potassium dihydrogen phosphate – 1.25g Water – 100ml Preparation of stains Phosphates are dissolved in freshly boiled water Azure B is grounded in mortar and added to phosphate solution so that it will be dissolved well Dyes are added in Phosphate solutions and mixed well and filtered Method Keep the smear for 1 second in Stain A Rinse gently in clean water for 5 – 10 seconds until the slide becomes free from stain Place the smear in solution B for 1 second Rinse for 2 to 3 seconds in clean water Place vertically in the rack and allow it to dry

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