Sterilization of Pharmaceutical Products.pptx

Sterilization sterility testing

1. PANDAVESWAR SCHOOL OF PHARMACY NAME : ATANU SARMA ROLL NO : 38405923011 COURSE : B.PHARM YEAR : 2 nd year, 3 rd semester SUBJECT : PHARMACUTICAL MICROBIOLOGY TOPIC : STERILIZATION TEACHER : AMITTABHA GHOSH

2. STERILIZATION INTRODUCTION PRINCIPLE APPLICATION PHYSICAL METHOD CHEMICAL METHOD GASEOUS METHOD RADIATION METHOD MECHANICAL METHOD CONCLUSION REFERENCE

3. INTRODUCTION Sterilization is a process, which make a substance free from all micro organisms both in vegetative and sporing states. By this process we can make a substance free from microbes.

4. PRINCIPLE In microbiology, sterilization is the process of removing all microorganisms from a surface or object, including vegetative and spore forms. The goal is to prevent microorganisms from growing on the object and make it sterile or aseptic. Sterilization is different from disinfection or sanitization, which only reduce microorganisms instead of eliminating them.

5. APPLICATION OF STERILIZATION FOODS : One of first step to word the sterilization was made by Nicols Apart who discover that through application of heat over suitable period. Medicine & Surgery : It is used to preparation of injectable medication. LABORATORY : It is used in labs to clean apparatus or glasswares .

6. PHYSICAL METHOD DRY HEAT STERILIZATION : All microbes including bacterial spores can be destroyed by heat. Dry heating at 100 c kill all vegetative bacteria in one hour but it does not kill spores. MOIST HEAT STERILIZATION : Microbes can be exposed to moist heat by using hot water, boiling water, steam at atmospheric pressure, steam under pressure (autoclaving) STERILIZATION BY RADIATION Sterilization by radiation also known as cold sterilization because no heat is used in this method. Electromagnetic waves which include infra red radiation, UV lights, X-ray & gamma rays.

7. CHEMICAL METHOD Chemical sterilization are that kind of sterilization where chemicals are involves the use of chemical agents to kill unwanted microorganisms such as fungi, bacteria & viruses and other disease causing agents . There are some example of chemical which is involved in chemical sterilization method – Alcohols Aldehydes Halogens Phenols, etc.

8. GASEOUS METHOD Gaseous sterilization method are that kind of sterilization where some gases are involved to kill the unwanted microorganisms from the substance. There are some example of chemical which is involved in chemical sterilization method – Ozone. Chlorine dioxide. Nitrogen oxide. Formaldehyde Gas, etc.

9. MECHANICAL METHOD Filtration is a mechanical method of sterilization that use filters with small process to separate microorganisms from liquids.

10. CONCLUTION Sterilization when properly used, disinfection & sterilization can ensure the safe use of invasive and non-invasive medical devices.

11. REFERENCE ‘ Internet ’ & the book ‘ PHARMACEUTICAL MICROBIOLOGY ’ which is written by ‘ N.K. JAIN ’ are help me to have an idea about this topic.

12. THANK YOU