Streptomycin- Pharmaceutical Microbiology

It is an antibiotic that was the first drug to be successful against tuberculosis but now it is used with other drugs because of its toxic effects
othere uses
Anti TB drug
Plaque
Veterinary treatment against gram negative bacteria in horses , cattle , sheep
Fermentation is defined as chemical transformation of organic compound brought about through agency of microorganisms


Microorganisms useful in fementation can be either prokaryotes such as bacteria , virus or eukaryotes such as fungi; yeast
Test Organism-Streptomyces griseus

1. STREPTOMYCIN BY- SANCHIT DHANKHAR

2. It is an antibiotic that was the first drug to be successful against tuberculosis but now it is used with other drugs because of its toxic effects

3. OTHER USES Anti TB drug Plaque Veterinary treatment against gram negative bacteria in horses , cattle , sheep

4. ADMINISTRATION Intravenously Intramuscularly{ in many countries it is only licensed to be used intramuscularly}

5. FERMENTATION Fermentation is defined as chemical transformation of organic compound brought about through agency of microorganisms Microorganisms useful in fementation can be either prokaryotes such as bacteria , virus or eukaryotes such as fungi; yeast

6. FERMENTATION OF STREPTOMYCIN Test Organism - Streptomyces griseus

7. REQUIREMENTS Substrate-glucose, fructose, galactose , xylose , mannitol , lactose , starch Source of nitrogen- peptone,meat extract, soy extract, the residues from alcohol distillation, dried pencillium mycelium, ammonium salts,nitrates , glycine

8. Growth stimulating agents-phenyl acetic acid, l – naphthalene Antioxidant-starch , agar , sodium sulphite

9. 3. PHASES first phase[2 days] consist of mycelial growth requirements oxygen, glucose, nitrogen, phosphorous pH rises to 8.0 due to production of ammonia

10. Second phase(1 to 2 days) In this streptomycin is produced without the production of mycelium Large quantities of glucose and oxygen are required Ammonia is utilized and ph falls to about 7.0

11. THIRD PHASE (1 TO 2 DAYS)

12. TYPES OF CULTURE METHOD IN FERMENTATION Surface culture method in which mold is grown on the surface of shallow layers of fermentation medium Submerged culture in which the mold is grown submerged in fermentation medium in shake flask or deep tanks

13. STREPTOMYCIN IS GENERALLY GROWN BY SUBMERGED CULTURE METHOD BY FOLLOWING WAYS MASTER STOCK CULTURE – The selected production strain is maintained in the form of master stock culture can be preserved by Freeze drying: By fixing spores in sterilized soil sand mixtures Storage under liquid nitrogen in frozen state Second method is simple and adequate and simple to adopt

14. Recovery Steps.. Master Culture Agar Slopes Shaker Flask Seed Vessel Fermenter Filtration Evaporator Crystallization Finishing

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