The principle and performance of liquid chromatography–mass spectrometry (LC-MS)�

The principle and performance of liquid chromatography–mass spectrometry (LC-MS)�

1. The principle and performance of liquid chromatography–mass spectrometry (LC-MS)

2. Liquid chromatography–mass spectrometry (LC-MS) an analytical chemistry technique that combines the physical separation capabilities of liquid chromatography (LC ) with the mass analysis capabilities of mass spectrometry (MS ) introduced in 1980s many compounds can be measured in a single analytical run very high sensitive and selective useful in many applications

3. Components

4. Liquid chromatography a) solvent inlet filter, ( b) pump, ( c) inline solvent filter, ( d) injection valve, (e) precolumn filter, (f) column, (g) detector, (h) recorder, (i) backpressure regulator, (j ) waste reservoir

5. Mass Spectrometry PHOTO Dario

6. Mass Spectrometry Ion generation Data analyzing Ions sorting by mass ( m/z ) Detection Solid Liquid Vapor

7. Ionization and Interface

8. Ionization and Interface Electrospray Ionisation (ESI )

9. Ionization and Interface Atmospheric Pressure Photo-ionisation Atmospheric Pressure Chemical Ionisation (APCI )

10. Ionization and Interface Thermospray Ionisation (TSI)

11. Ionization and Interface Particle Beam Ionisation (PBI)

12. Mass Analyzers A component of the mass spectrometer takes and separates ionized masses based on charge to mass ratios outputs them to the detector where they are detected and later converted to a digital output.

13. Mass Analyzers Quadrupole Analyzer

14. Time of Flight Analyzer Ion Trap Analyzer Mass Analysers

15. The ions - electrically detected by detector (have been separated according to their mass/charge ratio) The choice of detector is based on: the required detection sensitivity and the speed the thermal and chemical stability Detectors

16. Detectors Faraday cup detector

17. Detectors Photographic detector

18. Detectors Photomultiplier detector

19. Data Recording

20. Applications