This is the presentation report of fatty rizza

Presentation report in Science

1. Polyunsaturated fatty acids Cis configuration in nature Generally unsaturated fatty acids are confined to the sn-2 position of phospholipids and are 18-20 carbons long MUFA tend to be in the sn-1 position, like saturated fats. Hydrocarbon portion is saturated or partially unsaturated  3,6- PUFA  9 -MUFA

2. PUFA, MUFA Monounsaturated fatty acids (MUFA) - only one double bond present Polyunsaturated fatty acids (PUFA) - at least 2 double bonds are present Acetylenic – one or more triple bonds Polyunsaturated /Monounsaturated Ex. Docosahexaenoic Acid, Oleic Acid Double bonds have a planar conformation

3. MUFA Most of the 100 MUFAs identified are rare Oleic acid is very abundant in nature -most prevalent unsaturated fat in human diet -highest content in extra virgin olive oil (70g/ 100g)

4. 3 categories of PUFA Conjugated – if double bonds alternate with single bonds (– C = C – C = C –) Unconjugated – of the double bonds are separated by carbon atoms bonded with single bonds ( – C = C – C – C = C –) A third category exist in which double bonds are not all entirely in the methylene interrupted arrangement (present in certain microorganisms, marine lipids and some seed oils. Methylene interrupted

5. Unconjugated most common Subdivided into families, depending on the position of the double bond closest to the methyl side ( ), numbered 1 on the fatty acid chain. 12 omega families - 3,6,9 are what’s found in normal diet

6. -3 and -6 are Es sential Fatty Acids Mammals lack the enzymes to introduce double bonds at carbon atoms beyond C9. Hence , all fatty acids containing a double bond at positions beyond C9 have to be supplied in the diet. Linoleate (18:2 Δ 9,12) and Linolenate (18:3 Δ 9,12,15) are the two essential fatty acids in mammals . Interconversion between PUFAs occur only within the same family

7. Oleic Acid (OA): C18:1, n-9 or -9 Good source: Olive oil, Peanut oil, Soy oil Linoleic Acid (LA): C18:2, n-6 or -6. Essential Fatty Acid Alpha Linolenic Acid (ALA): C18-3, n-3 or -3. Essential Fatty Acid Good source: Flaxseed

8. Eicosapentaenoic Acid (EPA): C20:5, n-3 or -3. Essential Fatty Acid. Good source: Fish oil Docosahexaenoic Acid (DHA): C22:6, n-3 or -3. Essential Fatty Acid. Good Source: Fish oil Arachidonic Acid (AA): C20:4, n-6 or -6. Good source: Liver, Beef.

9. Omega-6 fatty acids Linoleic (C18:2) is essential Found in: -vegetable oil -nuts and seeds Only need about a tbsp /day

10. Omega-3 Fatty Acids primarily in fish and marine mammals Also found in soybean, canola, walnut, flaxseed oils Recommended omega6:omega-3 ratio is 2.3:1

11. Anthropological research suggest that our hunter gatherer ancestors consumed roughly 1:1 ratio of omega-6:omega 3. Vegetable oil consumption rose dramatically between the beginning and end of the 20th century. -Between 1935 and 1939, the ratio of n-6 to n-3 fatty acids was reported to be 8.4:1 -Ratio increased to 10.3:1 by 1985 -Todays estimates range between 10:1 to 20:1 Americans now get almost 20% of their calories from a single food source – soybean oil – with almost 9% of all calories from the omega-6 fat linoleic acid (LA) alone! recommended 30% of diet -10% monounsaturated -10% saturated -10% unsaturated

12. How Can Fatty Acids Modulate the Immune System? Membrane fluidity Lipid peroxidation inflammatory cytokines (IL-6, IL-12, IL-23 ) production Eicosanoid production Membrane fusion and phagocytic activity L ymphocytic proliferation I mmune cell migration Fatty acids impact:

13. Depending on diet, fatty acids end up in plasma membrane Consumption of hydrogenated oils results in reduction of PUFAs in plasma membrane Most tissues contain mainly omega-6 fatty acids (75% as LA and AA), with small amounts of omega-3 fatty acids -the exception is retina, brain and testes

14. 19 Eicosanoids : paracrine hormones Derived from the 20 carbon polyunsaturated fatty acid (arachidonic acid) Involved in reproduction, inflammation, fever and pain 3 classes: Prostaglandins, thromboxanes, leukotrienes Regulate synthesis of cAMP production  fever, pain, blood flow, and uterine contraction Produced by platelets, important in clotting and blood flow Inflammation, asthma, allergy

15. The Good, Reactive lipid species may benefit cell Fast & easy way for membrane to initiated a nonspecific response to external stress The Bad Reactive Lipid Species (RLS) are electrophilic in nature and capable of reacting with nucleophilic amino acids Thiol groups can act as a redox-switch controlling cell signaling, metabolism or gene expression & the G ooder RLS-mediated signaling is involved in various physiological pathways: apoptosis, induction of antioxidative defense, membrane repair, proteosomal pathways