Titration curve of amino acid by KK Sahu sir

Introduction of amino acid
Structure of amino acid
Classification of amino acid
Non-polar aliphatic ‘R’ group
Aromatic ‘R’ group
Polar uncharged ‘R’ group
Positively charged ‘R’ group
Negatively charged ‘R’ group
What is titration curve?
Amino acid act as acid and base
Curve of amino acid
Conclusions
References

1. TITRATION CURVE OF AMINO ACID By KAUSHAL KUMAR SAHU Assistant Professor (Ad Hoc) Department of Biotechnology Govt. Digvijay Autonomous P. G. College Raj-Nandgaon ( C. G. )

2. Introduction of amino acid Structure of amino acid Classification of amino acid Non-polar aliphatic ‘R’ group Aromatic ‘R’ group Polar uncharged ‘R’ group Positively charged ‘R’ group Negatively charged ‘R’ group What is titration curve? Amino acid act as acid and base Curve of amino acid Conclusions References S Y N O P S I S

3. INTRODUCTION OF AMINO ACID Amino acid are compounds containing amino group (-NH 2 ) and carboxyl group (COOH). So, amino acid are also called amino carboxylic acid. Amino acid are the essential component of all living cells. Amino acid are building blocks of protein. 20 amino acids are used as building blocks for the protein synthesis.

4. STRUCTURE OF AMINO ACID An amino acid is made up of 5 component namely:- A carbon atom- C A hydrogen atom- H An amino group- NH 2 A carboxyl group- COOH A side chain or residue- R

5. CLASSIFICATION OF AMINO ACID The amino acid can be classified by R group, they are 5 main classes- Non polar aliphatic R groups. Aromatic R groups. Polar uncharged R group. Positively charged R group. Negatively charged R group.

6. WHAT IS TITRATION CURVE? Titration is the analytical method used to determine the amount of acid in a solution. A measured volume of the acid solution is titrated by slowly adding a solution of base. Titration curves are obtained when the pH of given volume of a sample solution varies after successive addition of acid or alkali .

7. AMINO ACID ACT AS ACID AND BASE ZWITTER-ION :- zwitterions is a dipolar ion containing positive and negative charge .

8. TITRATION CURVE OF LYSINE

9. TITRATION CURVE OF ASPARTATE

10. TITRATION CURVE OF GLUTAMATE

11. TITRATION CURVE OF ALANINE

12. Conclusions:- Determine the titration curve for an amino acid and to use this curve to estimate the pKa value of ionizable groups of the amino acid and to understand the acid-base behavior of amino acid.

13. References:- Principles of biochemistry- David L. Nelson & Michael Cox (fifth edition) Biochemistry- Donald Volt Judith G. Voet ( 4 th edition) www.wikipedia.com