INTRODUCTION:
IOP is one of the major risk factor in:
Diagnosis
Prognosis
Only modifiable risk factor
Procedure performed to measure IOP - TONOMETRY
History of tonometry
1826: Concept started with William Bowman , he used digital
tonometry as a routine examination test.
1863: Albrecht von Grafe designed the first instrument to attempt
to measure intraocular pressure.
Further instruments followed, notably by Donders in 1865 and
Preistly-Smith in 1880.
These instruments were all of the indentation type and rested on
the cornea (no anaesthetic was used until 1884).
1885: Maklakov designed an applanation tonometer. This was
refined in 1892. Used for a number of years in Russia and Eastern
Europe. This was used till 1959.
1905: Hjalmar Schiotz produced his indentation tonometer. This
made tonometry a simple and routine clinical test.
Donders tonometer
Albrecht von Grafe tonometer
Ideal tonometer
Should give accurate and reasonable IOP measurement
Convenient to use
Simple to calibrate
Stable for day to day use
Easier to standardize
Free of maintenance problems
Types of tonometry
Direct – manometry
Indirect - A) Static
B) Dynamic
DYNAMIC TONOMETER
BALLISTIC TONOMETER: Vogelsang 1927 -rebound of a small
metal ball from the eye is measured and this depends to a
large extent on the physical properties of the coats of the eye.
VIBRATION TONOMETER: Roth & Blake 1963- Vibration tonometer
cause minimal deformation by oscillating force by a probe,
which also functions as a sensor and measures the resonant
frequency of the eye.
MANOMETRY:
Canula inserted into eye
Not practical clinically.
Intraocular pressure is higher than atmospheric pressure;
therefore, if a small hollow needle is inserted into the
anterior chamber, aqueous humor flows out through the
needle.
If the needle is attached to a
reservoir of fluid that is raised just
high enough to prevent any loss of
aqueous, the height of the column
of fluid, usually calibrated in cm of
water or mm of mercury, reflects
the intraocular pressure
DIGITAL TONOMETRY:
Intraocular pressure (IOP) is estimated by response of eye
to pressure applied by finger pulp.
indents easily – low IOP
Firm to touch – normal IOP
Hard to touch – high IOP
Schiotz tonometry-characteristics
The extent to which cornea is indented by
plunger is measured as the distance from the
foot plate curve to the plunger base and a
lever system moves a needle on calibrated
scale.
The indicated scale reading and the plunger
weight are converted to an IOP
measurement.
More the plunger indents the cornea, higher
the scale reading and lower the IOP
Each scale unit represents 0.05 mm protrusion
of the plunger.
PRINCIPLE
The weight of tonometer on the eye increases
the actual IOP (Po) to a higher level (Pt).
The change in pressure from Po to Pt is an
expression of the resistance of the eye (scleral
rigidity) to the displacement of fluid.
P(t) = P(o) + E
IOP with Tonometer in position Pt =
Actual IOP Po + Scleral Rigidity E
Determination of Po from a scale reading Pt
requires conversion which is done according to
Friedenwald conversion tables.
Friedenwald formula
Friedenwald generated formula for linear relationship
between the log function of IOP and the ocular
distension.
Pt = log Po + C ΔV
This formula has ‘C’ a numerical constant, the
coefficient of ocular rigidity which is an expression of
distensibility of eye. Its average value is 0.025
ΔV is the change in volume
TECHNIQUE
Patient should be anasthetised with 4%lignocaine
or 0.5% proparacaine
With the patient in supine position, looking up at a
fixation target while examiner separates the lids
and lowers the tonometer plate to rest on the
anesthetized cornea so that plunger is free to
move vertically .
Scale reading is measured.
The 5.5 gm weight is initially used.
If scale reading is 4 or less, additional weight is
added to plunger.
Conversion table is used to derive IOP in mm Hg
from scale reading and plunger weight.
SOURCES OF ERROR
Accuracy is limited as ocular rigidity varies from eye
to eye.
As conversion tables are based on an average
coefficient of ocular rigidity; eye that varies
significantly from this value gives erroneous IOP.
Repeated measurements lower IOP.
steeper or a thicker cornea causes greater
displacement of fluid during tonometry and gives a
falsely high IOP measurement.
Schiøtz reads lower than GAT
Low scleral rigidity
Low Scleral Rigidity
increasing age
high myopia
miotics
vasodilators
Postoperative after RD surgery (vitrectomy, cryopexy, scleral
band)
intravitreal injection of compressible gas.
keratoconus
Low ocular rigidity ----- falsely high scale reading -----
falsely low IOP.
LIMITATIONS
Instrumental errors
Standardisation - testing labs for certification
Mechanical obstruction to plunger etc.
Muscular contractions
Of extra ocular muscles increase IOP
Accomodation decreases IOP
Variations in volume of globe
Microphthalmos
High Myopia
Buphthalmos
It can be recorded only in supine position
Advantages of schiotz
tonometer
Simple technique
Portable
No need for SlitLamp or power supply
Reasonably priced
Calibration
The instrument should be calibrated before each use by placing
it on a polished metal sphere and checking to be sure that the
scale reading is zero.
If the reading is not zero, the instrument must be repaired.
Sterilization
The tonometer is disassembled between each use and the
barrel is cleaned with 2 pipe cleaners, the first soaked in
isopropyl alcohol 70 % or methylated spirit and the second
dry.
The foot plate is cleaned with alcohol swab.
All surfaces must be dried before reassembling.
The instrument can be sterilized with ultraviolet radiation,
steam, ethylene oxide.
As with other tonometer tips, the Schiotz can be damaged by
some disinfecting solutions such as hydrogen peroxide and
bleach.
GOLDMANN APPLANATION TONOMETER
GOLD STANDARD
Biprism
(measuring prism)
Feeder arm
Housing
Adjusting knob
Connects to the slit
lamp
Control weight insert
PRINCIPLE
Applanation tonometry is based on the Imbert-Fick
principle, which states that the pressure (p) inside an ideal dry,
thin-walled sphere equals the force (F) necessary to flatten its surface
divided by the area of the flattening (A).
P=F/A
Cornea being aspherical, wet, and slightly
inflexible fails to follow the law.
Moisture creates surface tension (S) or capillary
attraction of tear film for tonometry head.
Lack of flexibility requires force to bend the
cornea (B) which is independent of internal
pressure.
The central thickness of cornea is about 0.55
mm and the outer area of corneal flattening
differs from the inner area of flattening (A1). It is
this inner area which is of importance.
IMBERT FICKS LAW & MODIFIED IMBERT
FICKS LAW
W=PA W+S=PA
1
+B
Modified Imbert-Fick Law is
W + S = PA1 + B
When A1 = 7.35 mm
2
, S balances B and W
balances P.
This internal area of applanation is achieved
when the diameter of the external area of
corneal applanation is around 3.06 mm.
Grams of force applied to flatten 3.06
diameter of the cornea multiplied by 10 is
directly converted to mmHg.
cont…..
The two beam-splitting prism within the
applanating unit optically convert the circular
area of corneal contact in to semicircles
cont….
The instrument is mounted
on a standard slit lamp in such
a way that the examiners view
is directed through the centre
of a plastic Biprism.
Biprism is attached by a rod
to a housing which contains a
coil spring and series of levers
that are used to adjust the
force of the biprism against
the cornea.
Two beam splitting prisms
within applanating unit
optically convert circular area
of corneal contact in 2
semicircles.
procedure
The patient is asked not to drink alcoholic beverages as
it will lower IOP and not to take large amounts of fluid
(e.g., 500 ml or more) for 2 hours before the test, as it
may raise the IOP.
The angle between the illumination and the
microscope should be approximately 60°.
The room illumination is reduced.
A fixation light may be placed in front of the fellow
eye.
The tension knob is set at 1 g. If the knob is set at 0, the
prism head may vibrate when it touches the eye and
damage the corneal epithelium.
The 1 g position is used before each measurement.
Procedure cont..
The palpebral fissure is a little wider if the patient
looks up. However, the gaze should be no more
than 15° above the horizontal to prevent an
elevation of IOP.
After instilling topical anaestheia, Edge of corneal
contact is made apparent by instilling fluorescein
while viewing in cobalt blue light.
The biprism should not touch the lids or lashes
because this stimulates blinking and squeezing.
The patient should blink the eyes once or twice to
spread the fluorescein-stained tear film over the
cornea, and then should keep the eyes open wide.
Procedure cont..
In some patients, it is necessary for the examiner to
hold the eyelids open with the thumb and forefinger
of one hand against the orbital ring.
By manually rotating a dial calibrated in grams, the
force is adjusted by changing the length of a spring
within the device.
The prisms are calibrated in such a fashion that inner
margin of semicircles touch when 3.06 mm of the
cornea is applanated.
The Intra ocular pressure is then read directly from a
scale on the tonometry housing.
cont….
The fluorescent semicircles are
viewed through the biprism and the
force against the cornea is adjusted
until the inner edges overlap.
The fluorescein rings should be
approximately 0.25–0.3 mm in thickness – or
about one-tenth the diameter of the flattened
area.
Potential source of error
Thin cornea
Thick cornea
Astigmatism >3dioptre
Inadequate fluorescein
Too much fluorescein
Irregular cornea
Tonometer out of calibration
Elevating the eyes >15degree
Repeated tonometry
Pressing on the eyelids or globe
Squeezing of the eyelids
Observer bias(expectation and even numbers
Effect of central corneal thickness (CCT):
A thinner cornea may require less force to applanate
it, leading to underestimation of true IOP while a
thicker cornea would need more force to applanate
it, giving an artificially higher IOP.
The Goldmann applanation tonometer was designed
to give accurate readings when the CCT was 520 μm.
The deviation of CCT from 520 μm yields a change in
applanation readings of 0.7 mm Hg per 10 μm.
IOP measurements are also modified after PRK and
LASIK.
Thinning of the central cornea is gives lower readings
on applanation.
Wider mires or improper vertical alignment gives higher
IOP readings
If the two semicircles are not equal in size, IOP is
overestimated.
For every 3D increase in corneal curvature, IOP raises
about 1 mm Hg as more fluid is displaced under steeper
corneas causing increase in ocular rigidity
More than 6 D astigmatism produces an elliptical area
on applanation that gives erroneous IOP. 4D with-the-
rule astigmatism underestimate IOP and 4D against-the-
rule astigmatism overestimate IOP.
Mires may be distorted on applanating on irregular
corneas .
Elevating the eyes more than 15° above the
horizontal causes an overestimation of IOP.
Widening the lid fissure excessively causes an
overestimation of IOP
Repeated tonometry reduces IOP, causing an
underestimation of the true level . This effect is
greatest between the first and second readings,
but the trend continues through a number of
repetitions.
A natural bias for even numbers may cause slight
errors in reading.
Applanation- Possible Error
Falsely low IOP
too little flouroscein
thin cornea
corneal edema
with the rule astigmatism
1mm Hg per 4 D
prolonged contact
Repeated tonometry
Falsely high IOP
too much flouroscein
thick cornea
steep cornea
against the rule
astigmatism
1mm Hg per 3D
wider meniscus
Widening the lid fissure
excessively
Elevating the eyes more
than 15°
Potential Sources of Error – During Measurement
If the fluorescein rings are too wide, the patient’s eyelids should be blotted
carefully with a tissue, and the front surface of the prism should be dried with
lint-free material.
An excessively wide fluorescein ring can cause IOP to be overestimated
Potential Sources of Error – During Measurement
If the rings are too narrow, the patient should blink two or three times to
replenish the fluorescein; additional fluorescein may be added if necessary.
If the fluorescein rings are too narrow,IOP is underestimated.
Potential Sources of Error – During Measurement
Potential Sources of Error – During Measurement
Potential Sources of Error – During Measurement
Potential Sources of Error – During Measurement
Potential Sources of Error – During Measurement
Potential Sources of Error – During Measurement
Potential Sources of Error – During Measurement
CALIBRATION
GAT should be calibrated periodically, at least
monthly. If the GAT is not within 0.1 g (1 mmHg)
of the correct calibration, the instrument should
be repaired; however, calibration errors of up to
2.5 mmHg may still be tolerated clinically.
sterilization
Applanation tip should be soaked for 5-15 min in
diluted sodium hypochlorite, 3% H
2
O
2
or 70%
isopropyl alcohol or by wiping with alcohol, H
2
O
2
,
povidone iodine or 1: 1000 merthiolate.
Other methods of sterilization include: 10 min of
rinsing in running tap water, wash with soap and
water, cover the tip with a disposable film, and
exposure to UV light.
Disposable tonometer tips may also be used
When using disposable tips, they have a smooth
applanating surface.
The acrylic disposable tips seem to be somewhat more
accurate than the silicone ones.
While disposable shields or tips may be safer than
disinfection solutions, they are not 100% protective against
prion disease.
SAFETY REGULATIONS
No examination should be undertaken in case of
eye infections (or) injured corneas.
Only clean and disinfected measuring prism should
be used.
No damaged prisms should be used.
If the measuring prism come in to contact with
the cornea without the drum having previously
been correctly set, vibration can occur in the
feeler arm, which will produce unpleasant feeling
for the patient.
The tonometer tips should be examined
periodically under magnification as the antiseptic
solutions and mechanical wiping may cause
irregularities in the surface of the tip that can, in
turn, injure the cornea.
Perkins tonometer
It uses same prisms as Goldmann
It is counterbalanced so that tonometry is
performed in any position
The prism is illuminated by battery powered
bulbs.
Being portable it is practical when measuring
IOP in infants / children, bed ridden patients
and for use in operating rooms.
Draeger Tonometer
Draeger tonometer is similar to Perkins
It has a different set of prisms
It operates with a motor.
Mackay marg tonometer
Mackay-Marg Tonometer
This instrument is useful for measuring IOP in eyes
with scarred, irregular, or edematous corneas
because the end point does not depend on the
evaluation of a light reflex sensitive to optical
irregularity, as does the Goldmann tonometer.
It is accurate when used over therapeutic soft
contact lenses.
Tonopen
Tono pen
Portable
battery operated .
same principle as that of Mackay-Marg tonometer.
It is particularly useful in community health fairs, on
ward rounds ,children, irregular surfaces, measuring
through an amniotic membrance patch graft, to
read from the sclera .
Tono-Pen tends to overestimate the IOP in infants so
its usefulness in congenital glaucoma screening and
monitoring is somewhat limited.
In band keratopathy where the surface of the
pathology is harder than normal cornea, the Tono-
Pen tends to overestimate the IOP
A disposable latex cover which is discarded after
each use provides infection control.
Pneumatonometer or pneumatic
tonometer :
It is like Mackay-Marg tonometer.
The sensor is a air pressure like electronically controlled
plunger in Mackay-Marg tonometer.
It can also be used for continuous monitoring of IOP.
It gives significantly higher IOP estimates.
It has a sensing device that consists of a gas
chamber covered by a polymeric silicone
diaphragm.
A transducer converts the gas pressure in the
chamber into an electrical signal that is recorded
on a paper strip.
The gas in the chamber escapes through an
exhaust vent between the diaphragm and the tip of
the support nozzle.
As the diaphragm touches the cornea, the gas
vent is reduced in size, and the pressure in the
chamber rises.
Maklakov tonometer
•Indentation
•Can be used in
supine position
•wire holder
Dumb-bell-shaped
metal cylinders with
flat end plates of
polished glass
Diameter of 10 mm
The surface of the
weight is painted
with a dye, such as
mild silver protein
(Argyrol) mixed
with glycerin.
1 sec contact
imprint on end plate
IOP = W / π (d/2)
2
weight (W) diameter of the area of applanation (d)
Intraocular pressure is measured in grams per square
centimeter and is converted to millimeters of mercury
by dividing by 1.36.
Widely used in Russia and China
This instrument displaces a greater volume of
aqueous humor and thus IOP readings are more
influenced by ocular rigidity.
It does not correct for corneal bending, capillary
attraction, or tear encroachment on the layer of dye.
Many instruments similar to the Maklakow device have
been described,like the Applanometer, Tonomat,
Halberg tonometer, and GlaucoTest.
The Ocuton tonometer
The Ocuton™ tonometer
hand-held tonometer
works on the applanation principle
probe is so light that it is barely felt
needs no anesthetic in most patients.
It has been marketed in Europe for home tonometry
useful to get some idea of the relative diurnal variation in IOP if
the patient or spouse (etc.) can learn to use it.
Trans palpebral tonometry
used in situations where other, more accurate, devices are not practical,
such as in young children, demented patients and severely
developmentally-challenged patients.
In addition to all the problems facing indentation tonometry, such as
scleral rigidity, transpalpebral tonometry adds variables such as the
thickness of the eyelids, orbicularis muscle tone and potential Intra
palpebral scarring.
Portable so patients can measure their own IOP
at home, DVT.
It is not accurate always. Inter observer and
intra observer variability was large.subsequent
studies failed to confirm the accuracy of this
device.
Non contact tonometer
Noncontact tonometer (NCT) was introduced by Grolman.
Original NCT has 3 subsystems:
1. Alignment system: It aligns patient’s eye in 3 dimensions.
2. Optoelectronic applanation monitoring system:
It comprises transmitter, receiver and detector, and timer.
a. Transmitter directs a collimated beam of light at corneal
apex.
b. Receiver and detector accept only parallel coaxial rays
of light reflected from cornea.
c. Timer measures from an internal reference to the point of
peak light intensity.
3. Pneumatic system: It generates a puff of room air directed
against cornea
PRINCIPLE
A puff of room air creates a constant force that
momentarily flattens the cornea. The corneal
apex is deformed by a jet of air
The force of air jet which is generated by a
solenoid activated piston increases linearly over
time.
When the reflected light is at peak intensity, the
cornea is presumed to be flattened.
The time elapsed is directly related to the force
of jet necessary to flatten the cornea and
correspondingly to IOP.
The time from an internal reference point to the
moment of flattening is measured and
converted to IOP.
A puff of air of known area is generated against cornea
(B).
At the moment of corneal applanation,a light (T), which
is usually reflected from the normal cornea into space,
suddenly is reflected (R) into an optical sensor (A).
When the sensor is activated by the reflected light, the
air generator is switched off. The level of force at which
the generator stops is recorded, and a computer
calculates and displays the intraocular pressure.
NCT is accurate if IOP is nearly normal, accuracy
decreases with increase in IOP and in eyes with
abnormal cornea or poor fixation.
It is useful for screening programs because it can be
operated by non-medical personnel
It does not absolutely require topical anesthesia .
There is no direct contact between instrument and
the eye.
The patient should be warned that the air puff can
be startling.
The non-contact tonometer measures IOP over very
short intervals, so it is important to average a series of
readings.
New NCT, Pulsair is a portable hand held tonometer.
PULSAIR tonometer
Ocular Response Analyzer
It is an adaptation of the non-contact tonometer.
It directs the air jet against the cornea and measures not one
but two pressures at which applanation occurs
1) when the air jet flattens the cornea as the cornea is bent inward
and 2) as the air jet lessens in force and the cornea recovers.
Ocular response analyser
The first is the resting intraocular pressure.
The difference between the first and the
second applanation pressure is called
corneal hysteresis
corneal hysteresis is a measure of the
viscous dampening and, hence, the
biomechanical properties of the cornea.
The biomechanical properties of the cornea
are related to corneal thickness and
include elastic and viscous dampening
attributes.
IOP correlate well with Goldmann tonometry
but, on average, measure a few millimeters
higher.
Further , while IOP varies over the 24-hour day,
hysteresis seems to be stable.
Congdon et al found that a ‘low’ hysteresis
reading with the ORA correlates with progression
of glaucoma, whereas thin central corneal
thickness correlates with glaucoma damage.
It has practical value in the management of
glaucoma.
Dynamic contour tonometer
Introduced by Kanngiesser
It is based on a totally different concept other than
indentation or applanation tonometry.
Principle : By surrounding and matching the contour of
a sphere (or a portion thereof ), the pressure on the
outside equals the pressure on the inside.
The tip of the probe matches the contour of the cornea.
A pressure transducer built into the center of the probe
measures the outside pressure, which should equal the
inside pressure, and the IOP is recorded digitally on the
liquid crystal display (LCD).
The concept developed from a previous
contact lens tonometer called the ‘Smart Lens”.
It superior in accuracy to Goldmann tonometry
and pneumotonometry .
IOP is not affected by corneal thickness.
IOP is not altered by corneal refractive surgery
that thins the cornea.
Because the DCT measures IOP in real time, the
actual measurement, like the IOP, is pulsed. The
internal electronics ‘call’ the IOP as the bottom of
the pulsed curve and indicate it digitally on the
LCD ..
IOP readings with the DCT are generally lower than
GAT because, when properly done, indicates the
average difference between the maximum and
minimum pressures whereas the DCT reads the
minimum.
Ocular pulse amplitude
The DCT indicates the magnitude of the difference
between maximum and minimum IOP as the ocular
pulse amplitude.
OPA may be indicative of the status of ocular blood
flow and be differentially affected in different types
of glaucoma.
ocular pulse amplitude is
increased over normals in
most forms of glaucoma and
may be related to the level
of IOP.
ICARE tonometer
It is a new and updated version of an indentation
tonometer
Portable
can be used without anesthetizing the eye.
A very light, disposable, sterile probe is propelled
forward into the cornea .
The time taken for the probe to return to its resting
position and the characteristics of the rebound motion
are indicative of the IOP.
The time taken for the probe to return to its resting
position is longer in eyes with lower IOP and faster in
eyes with higher IOP.
It is comparable to the GAT.
It correlates with central corneal thickness like the
Goldmann, .
used in screening situations, when patients are
unable to be seated or measured at the slit lamp,
or when topical anesthetics are not feasible or
usable.
Not useful in scarred corneas (as does the
Goldmann).
Can be used with non-compliant patients (e.g.
children and dementia patients).
Continuous monitoring of intraocular pressure
Applanation instruments inside contact lenses or
suction cups or strain gauges in encircling bands that
resemble scleral buckling elements.
None of these instruments has achieved widespread
use.
resonance applanation tonometry measuring the
sonic resonance of the eye when a continuous force
over a fixed area is applied.
use of infrared spectroscopy to measure IOP.
To build a miniature pressure sensor that can reside
inside the eye; one such device is part of an
intraocular lens.
Tonometry for Special Clinical Circumstances
Tonometry on Irregular Corneas
The accuracy of Goldmann and Tono-Pen
tonometers and the noncontact tonometers is limited
in eyes with irregular corneas.
The pneumatic tonometer has been shown to be
useful in eyes with diseased or irregular corneas .
Tonometry over Soft Contact Lenses
Pneumo tonometry and the Tono-Pen can measure
with reasonable accuracy the IOP through bandage
contact lenses .
pneumotonometer correlates well with
manometrically determined IOP, whereas the Tono-
Pen consistently underestimates the pressure.
Tonometry with Gas-Filled Eyes
Intraocular gas affects scleral rigidity, rendering
indentation tonometry unsatisfactory.
pneumatic tonometer and Tono-Pen used.
A pneumatic tonometer underestimates
Goldmann IOP measurements in eyes with
intravitreal gas
Tono-Pen compares favorably with Goldmann
readings.
Both instruments significantly underestimated the
IOP at pressures greater than 30 mm Hg .
Tonometry with Flat Anterior Chamber
IOP readings from the Goldmann applanation
tonometer, pneumotonometer, and Tono-Pen
do not correlate well with manometrically
determined pressures.
Tonometry in Eyes with Keratoprostheses
In patients at high risk for corneal transplant
rejection, implantation of a keratoprosthesis is
now a viable option for vision rehabilitation .
Most keratoprostheses have a rigid, clear
surface, it is impossible to measure IOP by using
applanation or indentation instruments.
In such eyes, tactile assessment appears to be
the most widely used method to estimate IOP.
references
1) Shields glaucoma
2)Becker-Shaffers glaucoma
3) Diagnostic Procedures in ophthalmology Nema