transgenic-sheep-.pptx

transgenic-sheep-.pptx

1. Biotechnology Transgenic sheep Iv – semester PRESENTED BY SIVARAMAKRISHNAN.T II M.SC. MICROBIOLOGY Reg.no.20201232516121 PRESENTED TO DR.G.RAMANATHAN GANESHAN DEPARTMENT OF MICROBIOLOGY SRI PARAMAKALYANI COLLEGE ALWARKURICHI DEPARTMENT OF MICROBIOLOGY

2. Transgenic sheep 1996-DOLLY-the sheep was cloned using cells from a 6yr old sheep's udder by somatic cell nuclear transfer (SCNT)technology somatic cell nuclear transfer technology involves two different cells ovum somatic cell ( egg\oocytes) (skin fibroblast) -nucleus microinjected with nucleus transgenic DNA discarded cell enucleated egg discarded (empty ovum) lone somatic nucleus

3. Ovum containing somatic nucleus Embryo Implanted in the uterus of a female sheep Transgenic Animal Every cell containing the transgene

4. Sheep named by dolly was cloned by transfer of a nucleus from a mammary (udder) cell of an adult sheep in to an egg cell Mammary cell nucleus Insert in to a egg cell

5. First demonstration of pluripotency (totipotency) of a necleus of a differentiated adult cell Cloning of dolly Somatic cell nucleic Clone cattle Sheep Goats Pigs

6. Nuclear transfer procedures are similar Adult donor of cells from a variety of cells types Mammary epithelical Ovarian cells fibroblasts lymphocytes

7. Cultured and genetically modified method Adult cells Prolonged culture Experimenters have a additional time Successive genetic alteration

8. E.g. ; inactivating both alleles of a locus or creating multiple gene changes Individual donor cells are fused to an enucleated oocyte with short-duration electric pulse E.g.; two 2.5 kilovolt /cm pulse for 10microseconds Used to fuse adult cattle fibroblasts with enucleated oocytes The pulse simultaneously indce cell fusion and oocyte activation Blastocyst stage before transferred into the uterus of a pseudopregant female

9. Surviving animals produced by nuclear transfer are healthy There is a substantial loss of individual before and after birth some of the cloned animals display abnormalities Abnormliaties such as increased birth weight DNA methylation and histone modification of the original donor cell is inappropriate maintained in the cells of the recipient animal

10. USES OF TRANSGENIC SHEEP it is used as a model for studying Immunology Human Blood Clotting Factor Viii Transplantation Haematology Biological Product Manufacturing Recombinant DNA Drug Production In Milk

11. Advantages Site-specific Genetic Changes Are Possible All Offspring Are Transgenic In DNA Microinjection

12. Transgene Integration Occurs At Random Sites Unstable Things Are Formed Expression Is Always Constrained No.Of Generations Required Much Devoted Id Needed Nucleic From Somatic Cell Is Used Develop Perfect Cloning Of Livestock

13. DISADVANTAGES Difficult Procedure Failed In Vitro Fertilization Expensive

14. Industrial biotechnology and commodity products transgenic animals are being used for production of recombinant proteins in milk, egg, white, blood, urine seminal plasma ,and silk worm cocoons The amount milk produced by animals (L yr-1) are 8000 per cow, 1000 per goat, 300 per sheep, and per rabbit

15. Thank you