Types of recombinase

INTRODUCTION
DEFINITION
TYPES OF RECOMBINASES
Cre Recombinase,
Hin Recombinase,
Tre recombinase,
Rec A Recombinase,
CONCLUSION
REFERENCES

1. Types of Recombinases By KAUSHAL KUMAR SAHU Assistant Professor (Ad Hoc) Department of Biotechnology Govt. Digvijay Autonomous P. G. College Raj-Nandgaon ( C. G. )

2. SYNOPSIS INTRODUCTION DEFINITION TYPES OF RECOMBINASES Cre Recombinase Hin Recombinase Tre recombinase Rec A Recombinase CONCLUSION REFERENCES

3. INTRODUCTION A broad category of enzymes that are involved in the process of genetic recombination. DNA recombinases are widely used in multicellular organisms to manipulate the structure of genomes, and to control gene expression. These enzymes, derived from bacteria and fungi, catalyze directionally sensitive DNA exchange reactions between short (30–40 nucleotides) target site sequences that are specific to each recombinase .

4. DEFINITION Recombinases are genetic recombination enzymes. An enzyme that catalyzes the exchange of short pieces of DNA between two long DNA strands, perticularly the exchange of homologous regions between the paired maternal and paternal chromosomes. Those circuits depend on enzymes known as recombinases, which can cut out stretches of DNA, flip them or insert them.

5. Types of Recombinases These are the types of recombinases :- Cre Recombinase Hin Recombinase Tre Recombinase Rec A Recombinase

6. Cre Recombinase Cre recombinase is used as a tool to modify genes and chromosomes. Cre Recombinase is a tyrosine recombinase enzyme derived from the P1 Bacteriophage. The enzyme requires no additional cofactors or accessory proteins for its function. his 34 base pair loxP recognition site consists of two 13 bp palindromic sequences. The products of Cre-mediated recombination at loxP sites are dependent upon the location and relative orientation of the loxP sites.

7. HIN RECOMBINASE Hin recombinase is a 21 protein composed of 198 amino acids that is found in the bacteria Salmonella. Hin belongs to the serine recombinase family of DNA. Hin functions by binding to two 26bp imperfect inverted repeat sequences. Function of Hin recombinase

8. Tre Recombinase Tre recombinase is an experimental enzyme that in lab tests has successfully removed DNA inserted by HIV from infected cells. The enzyme was derived from Cre recombinase through selective mutation for the purposes of identifying HIV markers. function of Tre recombinase

9. Rec A recombinase RecA binds to a region of single-stranded DNA and catalyses the formation of duplex DNA. A recombinase recognizes and binds to each of two recombinant sites on different DNA molecules or within the same DNA. function of Rec A recombinase

10. FLP Recombinase The FLP protein of the yeast 2-microns plasmid catalyzes intermolecular site-specific recombination with a turnover number of approximately equal to 0.12 min-1 (per FLP monomer) for relaxed DNA substrates. Under conditions that enhance its stability, the protein can be used in catalytic rather than stoichiometric amounts. The reaction rate exhibits a strong dependence on FLP protein concentration even when the protein is present in excess relative to available recombination sites.

11. function of FLP Recombinase