Ultra performance liquid chromatography.pptx

Ultra performance liquid chromatography

1. Ultra Performance Liquid Chromatography (UPLC) Chromatographic and spectroscopic techniques

2. Ultra Performance Liquid Chromatography (UPLC)

3. Particulars Packing materials : < 2.5 µm High pressure : 50000 psi; 3447 Bar Volume injection : 2 µL Less solvent consumption Decrease run time Disadvantage : Due to increased pressure requires more maintenance and reduces the life of the column

4. CHARACTERISTICS HPLC UPLC Particle size 3-10 µm < 2.5 µm Pressure 3000-5000 psi; 344 Bar 50000 psi; 3447 Bar Column dimension 150 X 3.2 mm 150 X 2.1 mm Column temperature 30°C 65°C Volume injection 5 µL 2 µL Analysis time More Less Sensitivity Less More

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