UV Visible spectroscopy, BP701T. INSTRUMENTAL METHODS OF ANALYSIS

UV Visible spectroscopy
Electronic transitions, chromophores, auxochromes, spectral shifts, solvent effect on
absorption spectra, Beer and Lambert’s law, Derivation and deviations.
Instrumentation - Sources of radiation, wavelength selectors, sample cells, detectors- Photo tube, Photomultiplier tube, Photo voltaic cell, Silicon Photodiode. Applications - Spectrophotometric titrations, Single component and multi component
analysis

1. Uv visible spectroscopy

2. Introduction UV-Visible spectroscopy is an analytical technique used to measure the absorption of ultraviolet or visible light by a substance. It is widely used in both qualitative and quantitative analysis in various fields, including chemistry, biology, and pharmacy.

3. PRINCIPLE

4. Electromagnetic Spectrum:

5. Electromagnetic Spectrum:

6. INSTRUMENTATION

7. APPLICATIONS Qualitative Analysis: Identification of functional groups in organic compounds. Determination of molecular structure through electronic transitions (π → π*, n → π*). Quantitative Analysis: Determination of concentration using Beer-Lambert Law. Commonly used for the analysis of DNA, proteins, and pharmaceuticals.

8. ELECTRONIC TRANSISTION

9. DEFINATION An electronic transition refers to the movement of an electron from lower energy level (ground state) to a higher energy level (excited state). within an atom or molecule when it absorbs energy from ultraviolet (UV) or visible light.

10. (<200 nm)

11. (>200 nm)

12. σ π n π* σ* σ - σ * π - π* n - σ* n - π*