White Blood Cell count (WBC)

My report . (wbc count)

Report to practical physiology .
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University of AL_Ameed .
College of Dentistry .
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تقرير كامل ومفيد عن طريقة حساب عدد الكريات البيض تستطيع اعادة صياغته وتقديمه
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1. University Of Al-Ameed
College Of Dentistry

7. Lymphocytes NeutrophilsEosinophil Basophil Monocyte

10. Image of enlarged white blood cells,
which swallows a germ, as well as
the prominent part of the cartilage

12. LeukemiaNormal blood

14. a-Hemocytometercounting chamber (Neubauer,schamber).
b-WBCs counting pipette.
c-Rubber tube with a plastic mouth piece for drawing the fluid into a pipette.
d-Special hemocytometercoverslip
e-Diluting fluid.
f-Microscope.
Apparatus and Reagents

17. The area of each large corner square is 1 mm2, the depth of the chamber 0.1 mm (from chamber
surface to coverslip), the volume of blood in one square is 1 mm (length) ×1 mm (width) ×0.1 mm
(high) = 0.1 mm3 ,volume of four corner squares = 0.1 mm3 ×4 = 0.4 mm3 .
No. of cells in large corner square = N.
Dilution factor
No. of WBCs/mm3 = Cells counted ×———————
Volume factor
20
No. of WBCs/mm3 = N ×__________________ = N ×200
(In one square) 0.1
20
No. of WBCs/mm3 = N ×____________________ = N ×50
(In four squares) 0.4
Calculation

18. Adult 4,000-11,000 cell/mm3Infants 15,000-20,000 cell/mm3 Children 4,500-13,500 cell/mm3

19. WikipediaHealthline