5 1 cultivation of oyster mushroom

1. NME -BOTANY
-CULTIVATION OF OYSTER MUSHROOM
R. VELMURUGAN
Assistant Professor Of Botany
Saiva Bhanu Kshatriya College
Aruppukottai
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2. NME -BOTANY
-CULTIVATION OF OYSTER MUSHROOM
OYSTER MUSHROOM
PleurotusostreatusiscommonlyknownasOyster
Mushroom.TheFruitBodiesofthisMushroomaredistinctlyShell
Shaped.ItistheSecondMostCultivatedEdibleMushroomnext
toButtonMushroom.ItGrowsin20-30
0
CTemperatureandtheir
FruitingBodiesareNotEasytoAttackbyanyPathogensand
Pests.
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3. NME -BOTANY
-CULTIVATION OF OYSTER MUSHROOM
Cultivation of White Oyster Mushroom
Steps in the Cultivation of Mushroom
⚫Preparation of Spawn or Seed
⚫Preparation of Substratum or Bed
⚫Cultivation Method
⚫Aftercare
⚫Harvest and
⚫Storage.
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4. CULTIVATION OF OYSTER MUSHROOM
-PREPARATION OF SPAWN or SEED
PREPARATION OF SPAWN or SEED
⚫Wheat Seed with Fungal Mycelium called Spawn or Fungal Seeds.
⚫Wash the Wheat Seeds in Water.
⚫Dry the Seeds Under Shade.
⚫Boil the Seeds in Water for 50 minutes.
⚫Dry the Seeds in air by spreading on polythene sheet.
⚫Coat the Seeds with 2% Calcium Carbonate and 1 % Chalk Powder to
adjust the pH around 7 and also keeping the Seeds in individuals.
⚫Fill 250 g Seeds (Substrate) in Glass Bottle.
⚫Close the Mouth by Cotton Plug for Good Aeration and also Prevents
the Entry of Microbes.
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5. CULTIVATION OF OYSTER MUSHROOM
-PREPARATION OF SPAWN or SEED
•SterilizeinAutoclaveat121ºC
for30minutesandthenCool.
•InoculatethePureMyceliumof
Pleurotusostreatusintothe
BottleinLaminarFlowChamber.
•Incubateat25ºCfor10-12Days
forFungalGrowth.
•DuringIncubation,theMycelium
GrownintoDenseMyceliaon
WheatGrainstoformSpawn.
•TheSpawnisusedforthe
CultivationofMushroom.
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6. CULTIVATION OF OYSTER MUSHROOM
-PREPARATION OF SUBSTRATUM
PREPARATION OF SUBSTRATUM
OrganicWastethatusedforMushroomCultivationiscalledSubstratum
STEPSINSUBSTRATUM PREPARATION
⚫CutthePaddyStraworSugarcaneBagasseinto2.5cmSizePieces.
⚫TheSugarcaneBagasseisPerformedfortheBestProductionofOyster
MushroomwhencomparedtoPaddyStraw.
⚫SoakinWaterfor24Hours.
⚫BoilinWaterforOneHour.
⚫DryUndertheShadethePasteurizedSubstrates.ButMaintainsMoisture
Contentupto65–70%
⚫Spray10%CalciumCarbonateSolutionforMaintainthepHaround7.
⚫AddRiceBran,GypsumandMolassesForHighYield.
⚫Now,theSubstrateMixtureisReadyforMushroomCultivation.
⚫UsePolypropyleneBags(60X30cm)fortheCultivationofMushroom.
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7. CULTIVATION OF OYSTER MUSHROOM
-METHOD OF CULTIVATION
METHOD OF CULTIVATION
⚫TheCylindricalBlockSystemisBetterforOysterMushroom
Cultivation.
⚫TaketheSubstrateMixtureinPolypropyleneBagabout5cmHeight
asaFirstLayer.
⚫Spread50gmsofSpawnontheSubstratum.ThisProcesscalled
Spawning.
⚫MaketheSecondLayerofSubstratumabout10cmHeight.
⚫Spread50gmsofSpawnontheSubstratum,mostlyonthePeriphery.
⚫RepeatthisProcessforSeveralTimesintheSameManner.
⚫Finally,theSpawnCoveredwith5cmHeightofSubstratum.
⚫MakeManyHolesontheSurfaceoftheBagforWatering,Good
AerationandReduceTemperature.
⚫TietheMouthofBagwithThread.
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8. CULTIVATION OF OYSTER MUSHROOM
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9. CULTIVATION OF OYSTER MUSHROOM
-AFTERCARE
.
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AFTERCARE
•KeeptheCylindricalBlockBedonthe
RacksorinHangingPositionUnderthe
CultivationRoomorShed.
•SprinklingofWaterTwiceaDaywitha
VeryFineSprayer,keepingitsNozzle
UpsideandDropletsAllowedtoCome
DownVerySlowlyandMaintainthe
TemperatureBetween 20-30
0
Cand
Humidityabout80-85%.

10. CULTIVATION OF OYSTER MUSHROOM
-AFTERCARE
•SpawnGrows intoDense
MyceliuminsidetheBagwithin
10-12Days.
•After15days,Remove the
PolypropyleneBagandExpose
theBedinwhichMycelium
startstoDevelopintoKnots
calledPinHeadStage.
•After4-6Days,thePinHead
StageDevelopsintoShell
ShapedOysterMushroom.
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11. CULTIVATION OF OYSTER MUSHROOM
-HARVEST and STORAGE
HARVEST and STORAGE
⚫After21DaysofSpawning,startstoHarvesttheMushroom.
⚫HarvesttheMushroombyHandpickedMethod.
⚫HarvestMinimumofabout500to700kgofFreshOysterMushroom
fromOneTonofDryPaddyStrawin45-60Days.
⚫RemovetheDustParticlesfromMushroombySterilizedForceps.
⚫FreshMushroomshaveaShelfLifeof24-48HrsevenatRoom
Temperature
⚫TheOysterMushroomcanbeEasilytoDryandStoredinPowdered
FormforFutureUse.
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