7. Morphology of leishmaniasis parasites.ppt

VL

1. Morphology of Leishmania parasiteMorphology of Leishmania parasite

2. Morphology of Leishmania parasiteMorphology of Leishmania parasite
ObjectiveObjective
At the end of this session the participants At the end of this session the participants
will:-will:-
Understand the clear morphology of the Understand the clear morphology of the
parasite.parasite.
Know the staining characteristics of the Know the staining characteristics of the
parasite.parasite.
Differentiate the parasite from other Differentiate the parasite from other
parasites and artifacts.parasites and artifacts.

3. AmastigotsAmastigots
Are found in stained aspirations of Spleen, Are found in stained aspirations of Spleen,
Bone marrow, Lymph nods and Skin lesions of Bone marrow, Lymph nods and Skin lesions of
C.L.C.L.
Leishmania amastigotes are very small round Leishmania amastigotes are very small round
to oval organisms about 3-5 to oval organisms about 3-5 µms found inside µms found inside
or outside phagositic cells (macrophages).or outside phagositic cells (macrophages).

4. Each amastigote contains a red-mauve or Each amastigote contains a red-mauve or
pink nucleus, a smaller more deeply stained pink nucleus, a smaller more deeply stained
red-mauve kinetoplast and a pale blue red-mauve kinetoplast and a pale blue
stained cytoplasm.stained cytoplasm.
 The presence of the nucleus and The presence of the nucleus and
kinetoplast and a membrane is a particular kinetoplast and a membrane is a particular
diagnostic feature to look for in Leishmania diagnostic feature to look for in Leishmania
amastigotesamastigotes

5. AmastigotsAmastigots
Extracellular
Intracellular

6. PromastigotsPromastigots
Are found in infected female sand fly or can Are found in infected female sand fly or can
be harvested from cultures. be harvested from cultures.
They are spindle shaped and have nucleus, They are spindle shaped and have nucleus,
kinetoplast and a single terminal flagellum.kinetoplast and a single terminal flagellum.

7. PromastigotsPromastigots