Affinity chromatography

Affinity chromatography

1. Affinity Chromatography By...... Nandini Parkavi .S 15BBT028

2. Affinity Chromatography..... Separating biochemical mixtures based on a highly specific interaction between antigen and antibody, enzyme and substrate, or receptor and ligand .

3. Principle Stationary phase - mobile phase  The target molecule becoming trapped on a stationary phase  The other molecules in the mobile phase Elution

4. Affinity chromatography can be used to: Purify and concentrate a substance from a mixture into a buffering solution Reduce the amount of unwanted substances in a mixture Discern what biological compounds bind to a particular substance Purify and concentrate an enzyme solution.

5. Antigen - Antibody

6. Purification of mouse IgG1 Mouse IgG1 binds strongly to protein G

7. Polyclonal antibody