Agrobacterium mediated gene transfer

It shortly gives information about the Agrobacterium mediated gene transfer

1. AGROBACTERIUM MEDIATED GENE TRANSFER PRESENTED BY: SAKTHIVEL R 2014010016

2. Transformation Uptake of foreign DNA by plant cells is called transformation and these techniques are known as Transformation techniques .

3. It is a soil inhabiting bacterium and has Ti(Tumor inducing) plasmid. This bacterium invades crops such as tomato , sunflower , brinjal and cotton and causes crown gall disease which is in the form of tumerous growth . It is also has an ability to integrate new genetic material also called T-DNA or Transfer DNA into the plant cell Agrobacterium tumefaciens

4. Crown gall disease

5. A . tumefaciens

6. A Ti plasmid or tumour inducing plasmid is a plasmid that often ,but not always is apart of the genetic equipment that Agrobacterium tumefaciens use to transduce its genetic material to plants Ti Plasmid

7. T-DNA or Transfer -DNA

8. STEPS : Ti plasmid T-DNA

9. The explants of the plant must produce phenolic compounds ( e.g.acetosringtone ) for activation of virulence genes Transformed cells/Tissues should be capable to regenerate into whole plants Important Requirements :

10. This is a natural method of gene transfer. Agrobacterium can conveniently infect any explant (cells/tissues/organs) Even large fragments of DNA can be efficiently transferred. Stability of transferred DNA is reasonably good. Transformed plants can be regenerated effectively. Advantages

11. DISADVANTAGES: Limited host range . Can not infect cereal plants. Sometimes cells in a tissue that are able to regenerate are difficult to transfer.