AGROWASTE (agricultural waste i.e. agrowaste).pptx

AGROWASTE (agricultural waste i.e. agrowaste)

1. By Chellapandian Balachandran, Kathirvelu Baskar, Abeer Hashem, Elsayed Fathi Abd_Allah • Department of Biotechnology, Thiagarajar College, Tamil Nadu  • Department of Ecotoxicology, Ross Lifescience, Pvt. Ltd, Pune, India  • Botany and Microbiology Department, College of Science, King Saud University, Saudi Arabia. Publisher:-Elsevior Optimization of protease production from Bacillus halodurans under solid state fermentation using agrowastes  PRESENTED BY NAME- SHIBA MANDAL REG.NO-233001810195 ROLL NO-30099223034 STREAM-MSC BIOTECHNOLOGY UNDER THE SUPERVISION OF   DR.ARNAB KUMAR GHOSH  Department of Biotechnology MAKAUT,WB

2. Optimization of protease production from Bacillus halodurans under solid state fermentation using agrowastes  

3. OBJECTIVE The objective to study this topic is to establish an efficient ,cost effective, and sustainable production process for protease from Bacillus halodurans using agro-wastes as the substrate in solid-state fermentation.

4. Sample Footer Text INTRODUCTION Proteases belong to hydrolases (group 3). Proteases are the enzymes which break down the protein into smaller polypeptides or amino acids.  Protease are used in different industries such as textile, detergent, leather ,feed, waste and other.  The enzyme alkaline protease is composed of 60% of global enzyme market.  Among the bacterial strains, species of bacillus are notable producer of bioactive compounds in industrial sector.  The organism are subjected to protease production under solid state fermentation using different agrowastes (paddy husk, rice bran, fruit waste, wheat bran) as substrate.

5. MATERIAL & METHOD Sampling :       Marine sediment sample was collected and transported to the laboratory under aseptic condition. Screening of bacterial strains :      • The sediment sample was serially diluted and were plated on the skim milk agar for incubation (37 ⁰C) Characterization of the strain :      • To study the colony characteristics gram staining and biochemical tests were carried out for the characterization of the isolate.      • Genomic identification was done on the basis of 16s rRNA sequencing technique.

6. Solid state fermentation process  • 5 gm of wheat bran taken in flask and moisten with salt solution.  • Sterilized at 121⁰C for 15 min. • Inoculated with 1 ml of bacterial culture and incubated.  • Crude extract was obtained from culture medium by centrifugation which served as enzyme source.  Production optimization  • Fermentation process was carried out with various agro  wastes with different conditions such as pH, temperature, carbon, nitrogen sources , sodium chloride concentrations.  • Different pH was maintained using:- 0.2 M phosphate and phosphate-NaOH buffer. • carbon sources :- fructose, lactose, sucrose, cellulose, galactose, rhamnose ,and maltose.  • Nitrogen source:- ammonium sulphate, sodium nitrate, peptone, beef extract and skim milk powder were provided on the yield of protease. CONTINUE…

7. Colonies were screened for enzyme producing ability on skim milk agar. Among the four different isolates, one which produced maximum zone of clearance was subjected to further studies . The biochemical characteristics and the molecular sequencing revealed the organisms was phylogenetically related with B. halodurans . The clearing efficiency of the enzyme was found to be higher than commercial washing powder.  Fig:-Protease produce by Bacillus halodurans on the removal of blood stain   RESULT & DISCUSSION

8. CONTINUE… Fig : Optimization of protease production (U/ml) with different parameters by  Bacillus halodurans .

9. Several Bacillus strains such as B. licheniformis , B. subtilis , B amyloliquifaciens , and B. mojavensis have been identified as compare potential sources for the protease production.  Among the agrowastes tested wheat bran has yielded maximum protease .  The production of protease is affected by the factors such as pH and temperature in most of the organisms. The secretion of protease varies in microorganisms and it is dependent on environmental factors  The maximum protease production was recorded at pH 9 and incubation temperature 30 ⁰C.  Earlier studies reported that nitrogen sources for the highest protease production was registered with ammonium nitrate compared with other sources. In this study, the highest amount of protease yield by ammonium sulphate .  CONTINUE…

10. This study helps to conclude that isolated bacterial strain of Bacillus halodurans can be used for proteolytic activity by skim milk agar. The above study concluded that the isolated strains could be used as a promising agent for the protease production & making novel enzyme-based detergents “GREEN CHEMICALS” & strain removing purpose. Further studies and optimization could prove that protease to be very useful in industrial processes requiring protease or its hydrolytic products CONCLUSION

11. First and foremost, I'd want to thank my mentor, Dr. JAYA BANDHYOPADHYAY who served as an excellent role model over the duration of this work. Her advise and direction enabled me to reach my best potential. Also, I'd like to sincerely thank our respected teacher Dr. ARNAB KUMAR GHOSH and our Co- Ordinator Dr. MILON BANIK and other faculties for their contributions to the success of this presentation. Finally, I'd want to thank everyone who has assisted me and has always been at my side.Thank you so much for your continuous support and presence whenever needed. ACKNOWLEDGEMENT

12. 12 Sample Footer Text REFERENCE "Optimization of protease production from Bacillus halodurans under solid state fermentation using agro-wastes": https://doi.org/10.1016/j.sjbs.2021.04.069

13. Thank you Shiba Mandal [email protected] 13 Sample Footer Text