AJIT plant pathology culture media PDF.pdf

plant pathology

1.



WELCOME TO CULTURE
MEDIA
WELCOME.

2. 
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Students Name : MANE AJIT ANANDRAO
Reg. No. : ACR 062/2021
Course Title : Biofertilizer Production Technology And
Marketing.
Course no.: SRP- EL -PATH 407
Course Teacher : Dr. K.S.Ghutukade Sir.
STUDENT DETAILS

3. Affiliated to, Mahatma Phule Krishi Vidyapeeth , Rahuri
MOKASHI KRUSHI VIKAS PRATISHTHAN,
DADASAHEB MOKASHI COLLEGE OF
AGRICULTURE , RAJMACHI

4. Topic
Preparation Of Media For Bacterial
And Fungal Bio-inoculant

5. Micro – organism like all other living organism require basic 
nutrients for the sustenance of life.
Culture Medium -
"The Food material on which micro – organism are grown in 
the laboratory is known as culture medium ."

6. Major Contribution
Robert Koch
                         He was German scientist who discovrde
 causative agent for deadly diseases like Tb, cholera, 
anthrax. He discovered solid culture media & was the first 
to obtain pure culture of bacteria.
Louis Pasteur  
The first liquid artificial culture medium was created in
1860.

8. Uses Of Culture Media : 
To identify the cause of infection from the clinical sample
so that the proper treatment can be given.
To study the characteristics or properties of micro-
organisms.
To prepare biological products like vaccines , Toxoides,
antigens.

9. Need for Culture Media

- It is usually essential to obtain a culture by growing the organism in an
artificial medium .

-If more than one species or type of organism are present each requires
to be carefully separated or isolated in pure culture.

- Several organism need the determination of antibiotic sensitivity
pattern for optimal antibiotic selection .

10. •
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Characteristics of good ideal medium



Must give a satisfactory growth from a small inoculation.

Should promote a rapid growth

should be easy to prepare

should be reasonably cheap

should be easily reproducible

11. •
Preparation and preservation of culture media :

•Generally dehydrated culture media are prepared in microbiological
laboratories in systemic manner and with proper skills .

•They should be weighted properly and dissolved homogenously

The pH is adjusted as per requirements after preparation

•Most culture media are sterilized by autoclaving.

•Certain liquid media placed in screw capped bottles or tubes / cotton
plugged can be kept at room temperature for weeks .

12. Composition Of Culture Media :
1.Agar
2.Peptone
3.Extract (Meat extract , Yeast extract )
4.Carbohydrates.
5.Water
6.Mineral salt

13. Bioinoculants or microbial inoculants :  
Bioinoculant are the beneficial soil amendments that use
microbes for promoting plant growth & development.
Microbial inoculants refer to formulations composed of
beneficial micro – organism (bacteria, fungi, viruses &
algal ) that play an important role in soil ecosystem for
sustainable agriculture. 
Microbial inoculants are able to carry out the plant
growth promoters ( Biofertilizers ), plant disease control
(Biocontrol agents - fungicides)
It has the ability to minimize the negative impact of
chemical input & increase quantity & quality of
agriculture product.

14. Microbial inoculant are eco- friendly & deliver plant
nutrients to plants in a more sustainable manners

15. Types Of Microbial Inoculants : 
1.Nitrogen Fixing bacteria –  Azospirillum, Rhizobium, 
Azotobacter, Acetobacter, Frankia.
2.Phosphate Solubilizer – Pseudomonas spp,
Bacillus Sp.
3.Phosphate Mobilizer – Mycorrhizal fungi.

16. Following are the selective media for different 
organism : 
Bacteria :For
Rhizobium – Congo Red Yeast Extract Mannitol Agar.
Azotobacter - Ashby's media & Jensen's broth.
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Azospirillum – Semisolid NFB media
PSB – Pikovaskayas media.
Acetobactor – Gluconacetobacter.
KSB – Glucose Yeast agar 
Pseudomonas - King's B media
BGA - Fogg's media.

17. For Fungal :
Trichoderma- Potato dextrose Agar Media

18. CLASSIFICATION OF
CULTURE MEDIA

19. Culture media has been classified variously using different
criteria like chemical composition, physical state & Function.
Culture Media
1.Composition
2.Physical
state
3. Function.
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Natural culture
media
Semi synthetic
media
 Synthetic
media
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Solid media 
Liquid media
Semi solid
media
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Enriched media
Selective media
Differential
media
Transport media

20. 1.Based on Composition
A.
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Natural culture Media - 
The exact chemical composition of natural medium
is not known.
The natural media include milk, urine, diluted
blood, vegetable juices, meat extract & peptones.

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Semi synthetic media -
Those media whose chemical composition is
partially known are called semi synthetic media.
Any medium which contains agar becomes a semi-
synthetic medium. 
Ex: PDA, oat meal agar, beef peptone and nutrient
agar are some of the semi- synthetic media.
Beef Peptone

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Synthetic or chemically defined media
Composed of special substances of well known
composition.
Synthetic medium may be a general purpose
medium used for a wide variety of micro-organism
Ex….Richard media, Peptone water, corn media.

23. 2. Based on physical state
A.
B.
C.
Solid media
Liquid media
Semi-solid media
A.
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Solid media - 
Media which are solid in state. 
Agar is the major solidifying
agent, added in concentration
of 1.5% for plates, slant media.
Ex. Nutrient Agar ,Macconkey
Agar

24. 
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. Liquid media -
Media which are in Liquid
state.
This form of media don't
contain agar or any solidifying
agent. Referred as Broth.
Ex. Nutrient broth, Macconkey
broth.

25. 
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. Semisolid media -
This form of medium is
prepared by adding a small
amount of agar (0.2-0.5gm) to
a fluid medium.
The consistency of this media
is , the consistency between
solid & liquid.
Ex. Hugh Leifsons media, SIM
media.

26. 3.On the basis of function
A.
B.
C.
D.
Enriched media
Selective media
Differential media
Transport media
A.
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Enriched media - 
Substances like blood, serum, egg are added to the simple
medium.
Used to grow bacteria that are exacting in their nutritional need
Ex. Blood agar, Chocolate agar.

28. B.Selective media.
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The inhibitory substance is added to a solid media to
 inhibit commensal or contaminating bacteria such as : 
Antibiotics
Dyes
Chemicals
Alteration Of pH.

29. C .Differential Media
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a.
b.
c.
d.
It is designed in such a way that different bacteria can be
recognized on the basis of their colony colour.
Dyes & metabolic substances are incorporated so that
those bacteria that utilize them appear as differently colored
colonies.
Examples :
Macconkey Agar.
Cysteine Lactose Electrolyte Deficient Agar.
Thiosulfate Citrate Bile Salts Sucrose Agar.
Xylose Lysine Deoxycholate Agar.

30. a.Macconkey agar medium 
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Distinguish between lactose fermentors &
non lactose fermentors.
Lactose fermentors – Pink colonies.
Non lactose fermentors – colorless colonies.
b. Xylose Lysine
Deoxycholate Agar (XLD
media )

It is used for the recovery of
salmonella spp. & Shigella spp.

31. D. Transport Media
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Media used for transporting the
samples.
Delicate organism may not
survive the time taken for
transporting the specimen
without a transport media.
Ex. Stuart's medium.

32. THANK YOU.