An depth look about 17alpha-methyltestosterone uses in Tilapia sex reversal.pdf

17alpha-Methyltestosterone is a versatile synthetic androgen and anabolic steroid with applications ranging from medical treatments to bodybuilding and aquaculture. In medicine, it helps treat hormone deficiencies and specific cancers. In non-medical contexts, it is used illicitly for enhancing athletic performance. In aquaculture, it plays a crucial role in producing all-male fish populations to optimize growth and productivity.

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Andepthlookabout17alpha-methyltestosteroneusesinTilapia
sexreversal

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Introduction.......................................................................................................................................................................................................3
Whatismethyltestosterone?..........................................................................................................................................................................4
Methyltestosteronepowdersynthesis..........................................................................................................................................................6
HowdoesMethyltestosteronework?............................................................................................................................................................7
Whatcan17alphamethyltestosteronepowderusefor?..........................................................................................................................8
Methyltestosteronepowderuseinbodybuilding..............................................................................................................................10
17-alphamethyltestosterone(MT)powderuseinTilapiafish:.......................................................................................................11
Whatisthepriceof17-methyltestosteronepowder?...............................................................................................................................12
Howtouse17-methyltestosterone(MT)powderfortilapia?Dosageguide.........................................................................................13
Whatisdifferencebetweentestosteroneandmethyltestosterone?......................................................................................................15
Whatismethyltestosteroneanabolicratio?...............................................................................................................................................17
Whydoathletesusemethyltestosterone?.................................................................................................................................................17
Ismethyltestosteronelegal?........................................................................................................................................................................18
Methyltestosteronepowderforsale-wheretobuypure17-MTpowder?...........................................................................................19

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Introduction
Haveyoueverwonderedhowcertainsubstancescanenhanceathleticperformance,aidinmedicaltreatments,
andevenplayaroleinaquaculture?Onesuchmultifacetedcompoundis17-alpha-methyltestosterone.Known
scientificallyas17α-methyl-4-androsten-17β-ol-3-one,thissyntheticandrogenandanabolicsteroidhasawide
rangeofapplications,fromtreatingmedicalconditionstoboostingmusclegrowthinbodybuildersandaidingin
fishfarming.
Firstsynthesizedinthe1930s,17-alpha-methyltestosteronehasstoodthetestoftimeduetoitspotenteffects
andversatility.Medically,ithasbeenusedtoaddressconditionslikehypogonadismanddelayedpubertyinmales,
andevencertaintypesofbreastcancerinwomen.Beyondthemedicalfield,thiscompoundispopularamong
athletesandbodybuildersforitsabilitytoenhancemusclemassandstrength.Interestingly,italsoplaysacritical
roleinaquaculture,particularlyinthesexreversaloftilapiatooptimizeproduction.

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Whatismethyltestosterone?
17α-Methyltestosterone(MT),alsoknownas17-alpha-Methyltestosterone,17α-MT,methyltest,oras
17α-methylandrost-4-en-17β-ol-3-one,isasynthetic,orallyactiveandrogenic-anabolicsteroid(AAS)usedto
treatmaleswithandrogendeficiency.Itisa17α-methylatedderivativeoftestosteronethatisgivenorallyin
capsuleandtabletform.Thismodification,whichinvolvesaddingamethylgroupatthe17thcarbonposition,
enhancesitsbioavailabilityandmetabolicstability.
Methyltestosteronefunctionsasanagonistoftheandrogenreceptor,exertingbothandrogenicandanabolic
effectsinthebody.Itschemicalstructureincludesahydroxylgroupatthethirdcarbonandaketonegroupatthe
sameposition,similartonaturaltestosteronederivatives.However,duetoitsefficientaromatizationintothe
potentestrogenmethylestradiol,methyltestosteronehasrelativelyhighestrogenicity,leadingtopotentialside
effectssuchasgynecomastia.

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TherearecurrentlyfourFDA-approvedmethyltestosteronemedications,thebrandnameareAndroid,Metandren,
Testred,Virilon.Thesebrand-namedrugsareusedforvariousmedicalpurposessuchastreatinglow
testosteronelevelsinmen,delayedpubertyinboys,andcertainsymptomsofmenopauseinwomen.Theyare
availableindifferentforms,includingoraltabletsandcapsules.
Theactiveingredientsofthesemedicationsaremethyltestosteronepowder.Allfourmedicationsareonly
availablewithaprescription.Forpharmacies,pharmaceuticalcompanywhowanttocompoundthemedications
andbuildyourownbrand,buyingpuremethyltestosteronerawpowderwholesalefromAea.ltdwillgetafactory
price.

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Methyltestosteronepowdersynthesis
Thesynthesisof17alpha-methyltestosteronepowderwasfirstproducedbyErnstLaqueur,JacobF.Haslinger,
andtheircolleaguesin1935.Thisdevelopmentmarkedasignificantadvancementinthefieldofsynthetic
androgensandanabolicsteroids.
Methyltestosterone(17α-Methyltestosterone)synthesisinvolvesseveralchemicalreactionstomodifythe
structureoftestosterone.Thekeymodificationistheadditionofamethylgroupatthe17thcarbonposition,
enhancingitsoralbioavailabilityandstability.Thesynthesisprocessincludesprotectingthehydroxylgroupof
testosterone,introducingamethylgroupatthe17αposition,andthendeprotectingthemoleculetoyield
17α-Methyltestosterone.Thisprocessrequirescarefulhandlingofreagentsandprecisecontrolofreaction
conditionstoachievethedesiredproductwithhighpurityandyield.Asthesynthesisprocessof

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methyltestosteronepowderisreportedbymoreandmoremanufacturers,leadtomoreandmoreprofessional
manufacturersandlaboratoriestoproduce17-alpha-methyltestosteronepowder.
17a-methyltestosteronepowderisasyntheticsteroidrawpowder,methyltestosteronemedicationsisprimarilyfor
oraladministrationandItisavailableinAea.ltdinrawpowderform.
HowdoesMethyltestosteronework?
Methyltestosterone,analphaalkylatedsteroid,worksbybindingtoandrogenreceptors(AR),initiatingthe
transcriptionofandrogen-responsivegenesresponsibleforsignificantgrowthandthedevelopmentofsecondary
sexualcharacteristics.Itactsasasubstrateforthe5α-reductaseenzyme,transforminginto
17α-methyl-dihydrotestosterone(mestanolone).Thisbindingandsubsequentgeneactivationenhanceprotein
synthesis,musclegrowth,andbonedensity.

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Unlikeothertestosteronederivatives,17-Methyltestosteronehasaloweranabolicactivityrating,resultingina
smalleranabolictoandrogenicratioandrelativelyhigherandrogenicproperties.However,acriticaldownsideis
thatitaromatizesintoamorepotent17α-methylestradiolduringitsactioninthebody.Thisaromatizationtriggers
estrogenicity,leadingtoestrogenicsideeffectssuchaswaterretentionandgynecomastia.
Thestructuralmodificationatthe17thcarbonpositionincreasestheoralbioavailabilityandmetabolicstabilityof
methyltestosterone.Despiteitsbenefits,thepotentialsideeffects,includingestrogeniceffectsandlivertoxicity,
mustbeconsidered.
Whatcan17alphamethyltestosteronepowderusefor?
Methyltestosteroneandethyltestosterone(17α-ethyltestosterone)aretheparentstructuresofall17α-alkylated

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AAS.Major17α-alkylatedAASincludethetestosteronederivativesfluoxymesterone,metandienone
(methandrostenolone),andmethyltestosteroneandtheDHTderivativesoxandrolone,oxymetholone,and
stanozolol.Althoughitisnotcommonlyused,theactiveingredientofMethyltestosteronepowderhasdiverse
applicationsinmedicaltreatmentsandbodybuilding.Now17alphamethyltestosteronepowderaremorepopular
insexreversalpurposeforTilapia.Herearesomemethyltestosteroneuses:
MethyltestosteronepowderforMedicaluse
HormoneReplacementTherapy(HRT):Inthepast,methyltestosteronewasusedtotreatconditionssuchas
hypogonadism(lowtestosteronelevels)inmen.Itwasadministeredtosupplementorreplacetestosteronewhen
thebodywasunabletoproducesufficientamountsnaturally.
DelayedPuberty:Methyltestosteronehasbeenusedtostimulatepubertyinadolescentboyswhoexperience
delayedpuberty.Byprovidingexogenousandrogens,ithelpsinducethedevelopmentofsecondarysexual

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characteristics,suchasdeepeningofthevoiceandgrowthoffacialandbodyhair.
MenopausalHormoneTherapy:Atlowdoses,methyltestosteronehasbeenusedasacomponentofhormone
replacementtherapy(HRT)formanagingmenopausalsymptomsinwomen,suchashotflashes,osteoporosis,
andlowsexualdesire.However,itsuseforthispurposehasdecreasedovertimeduetosafetyconcernsandthe
availabilityofalternativetherapies.
BreastCancerTreatment:Insomecases,methyltestosteronehasbeenusedinthetreatmentofadvancedbreast
cancerinpostmenopausalwomen.Itmaybeprescribedaspartofahormonetherapyregimentosuppress
estrogenlevels,whichcanhelpslowthegrowthofhormone-sensitivebreasttumors.
Methyltestosteronepowderuseinbodybuilding
Outsideofmedicalsettings,17a-Methyltestosterone(MET)powderissometimesusedbyathletesand

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bodybuildersasaperformance-enhancingdrug.Itisbelievedtoincreasemusclemass,strength,andendurance,
whichcanhelpathletesimprovetheirathleticperformance.However,methyltestosteroneforbodybuildingisnot
aspopularasothertestosteroneestertoday,oneofreasonismethyltestostoeroneistakenorally,itwillincrease
moreriseofthelivertoxicitythanotherinjectiontestosterone.
17-alphamethyltestosterone(MT)powderuseinTilapiafish:
Oneofthesignificantapplicationsof17
alpha-Methyltestosteronepowderisinaquaculture,itis
mainlyusedashormoneforsex-reveralinfry.Sex
reversalisatechniqueusedtoproducemonosex
populations,typicallyall-malepopulations,whichcangrow
fasterandlargerthanmixed-sexpopulations,particularly

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intheproductionoffishlikeNiletilapia(Oreochromisniloticus).Byfeedingthefishwithmethyltestosterone
powder-treatedfeedduringtheearlystagesofdevelopmenttopromotemalecharacteristics,improvinggrowth
ratesanduniformitywhichcanbedesirableforcommercialtilapiafarming.
However,theuseof17MThormoneinaquacultureissubjecttoregulationsandguidelines,anditsusemustbe
carefullymanagedtoensureenvironmentalsustainabilityandfoodsafety.
Whatisthepriceof17-methyltestosteronepowder?
Typically,thecostforasupplyof100tabletsofmethyltestosterone10mgcanrangefrom$60to$150ormore,
dependingonthepharmacy.Brand-nameversionscancostmore,oftenrangingfrom$100to$300forasimilar
supply.Astheactiveingredientraw,youcanbuy17-methyltestosteronepowderonlinewithcheaperprice.Oneof
themostcommonmethodsforcommercialproductionofall-maletilapiapopulationsistouse17alpha
MethyltestosteronePowder(MT).Inordertoreducethecostoffarming,itwillbeagoodideatobuy
17-MethyltestosteronePowderwholesale.Buy17a-MethyltestosteronePowderbulkfromAea.ltdmanufacturer

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willgetafactoryprice.
Howtouse17-methyltestosterone(MT)powderfortilapia?Dos
ageguide.
Aea.ltdasa17alpha-methyltestosteronepowdersupplier,thedosagesupplyhereisonlyforreference.Here
belowaregeneralfarmingexperiencessharedbyourcustomers:
Usingmethyltestosterone,specifically17alpha-methyltestosterone(17α-MT),forsexreversalintilapiarequires
precisepreparationandadministrationtoachieveeffectiveresultswhileminimizingrisks.Theprocessbegins
withthepreparationofhormone-treatedfeed.Thetypicaldosagerangesfrom40to80mgof17α-MTper
kilogramoffeed,with60mgperkgbeingacommonstandard.Thehormonepowderisfirstdissolvedinasmall
amountofethanolorisopropanoltoensureevendistribution.Thissolutionisthenthoroughlymixedwiththefish

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feedandallowedtoairdrytopreventmoistureretention.
Feedingshouldstartsoonafterthefryabsorbtheiryolksac,usuallyat3-10daysold,andcontinuefor21to28
daystoensureeffectivesexreversal.Thefryshouldbefedmultipletimesaday,typically3-4times,toensure
adequateintakeofthehormone-treatedfeed.Duringthisperiod,thefryarehousedinclean,well-aerated
aquariumsortankswithoptimalwaterqualityparametersmaintained,includingtemperature,pH,anddissolved
oxygen.Stockingdensitiesshouldbemanagedcarefully,usuallybetween500-1000fryperliter,tominimize
stressandpromoteuniformgrowth.
Afterthehormonetreatmentperiod,thefryaretransferredtonurseryhapasornetsinearthenpondsata
stockingdensityofaround1000fishpersquaremeter.Theyarethenswitchedtohigh-quality,regularcommercial
feed.Regularmonitoringofthefryisessentialtoobservetheirhealth,growth,andanysignsofstressorillness.
Sexverificationisrecommendedafterafewweekstoassessthesuccessofthesexreversalprocess.

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Safetyprecautionsarecriticalwhenhandlingmethyltestosterone.Alwaysuseglovesandprotectivegeartoavoid
directcontactwiththehormone.Properdisposalofanyleftoverhormone-treatedfeedisnecessarytoprevent
environmentalcontamination.Compliancewithlocalregulationsandguidelinesontheuseofhormonesin
aquacultureensuressafeandresponsibleapplication.Thismethodofusingmethyltestosteronehelpsachieve
predominantlymaletilapiapopulations,whicharepreferredfortheirfastergrowthanduniformsize.
Whatisdifferencebetweentestosteroneandmethyltestosteron
e?
Testosteroneandmethyltestosterone,thoughbothbelongingtotheclassofanabolic-androgenicsteroids(AAS),
exhibitsignificantdifferencesintheirchemicalstructure,administrationmethods,effects,andmedicaluses.
Testosterone,anaturalhormonepredominantlyproducedinthetestesinmenandtoalesserextentinthe

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ovariesinwomen,hasabasicstructurerepresentedas17β-hydroxyandrost-4-en-3-one.Conversely,
methyltestosterone,asyntheticderivativeoftestosterone,isstructurallymodifiedwiththeadditionofamethyl
groupatthe17thcarbonposition,renderingitsstructureas17α-methyl-17β-hydroxyandrost-4-en-3-one.This
modificationenhancesmethyltestosterone'soralbioavailability,allowingittobeadministeredorallyinpillform,
whereastestosteroneisprimarilyadministeredviainjectionortransdermalmethodsduetoitspoororal
bioavailability.Whilebothcompoundsareutilizedfortreatingconditionssuchaslowtestosteronelevelsand
delayedpuberty,methyltestosteroneisoftenpreferredfororaladministration,despiteitsheightenedriskofliver
toxicityandestrogenicsideeffects,whicharelesspronouncedwithtestosterone.Additionally,testosterone's
balancedanabolictoandrogenicratiomakesitmoreeffectiveforpromotingmusclegrowthanddevelopmentof
malesecondarysexualcharacteristics,distinguishingitfrommethyltestosterone,whichtendstoexhibitslightly
higherandrogenicpropertiesrelativetoitsanaboliceffects.

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Whatismethyltestosteroneanabolicratio?
Methyltestosteronehasarelativelyloweranabolicactivitycomparedtoitsandrogenicactivity.
Specifically,theanabolictoandrogenicratioofmethyltestosteroneisapproximately1:1.Thismeansthatits
anaboliceffects(suchasmusclegrowthandincreasedstrength)andandrogeniceffects(suchasdevelopmentof
malesecondarysexualcharacteristics)arebalanced.However,duetoitssignificantandrogenicactivity,itisless
commonlyusedforpurelyanabolicpurposescomparedtootheranabolicsteroidspowdersuchastestosterone
enanthatepowder,testosteronecypionatepowder.
Whydoathletesusemethyltestosterone?
Athletessometimesusemethyltestosteroneforitsanabolicandandrogeniceffects,whichcanenhancetheir
physicalperformanceandappearance.However,Methyltestosteronebegantofalloutoffavorinthebodybuilding

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communityprimarilyduringthe1980sand1990s.Oneofthereasonsisduringthe1980sand1990s,the
developmentandavailabilityofotheranabolicsteroidswithmorefavorableanabolictoandrogenicratios,suchas
oxandrolone(Anavar),stanozolol(Winstrol),andmethenolone(Primobolan),providedbodybuilderswithoptions
thathadlowerincidencesofseveresideeffects.Nowmethyltestosteroneismainlyuseinfishfeed.
Ismethyltestosteronelegal?
Thelegalityofmethyltestosteronedependsonthespecificjurisdictionanditsintendeduse.Inmanycountries,
methyltestosteroneisacontrolledsubstanceduetoitsclassificationasananabolic-androgenicsteroid(AAS).In
medicalsettings,itmaybelegallyprescribedbyhealthcareprofessionalsfortreatingconditionssuchaslow
testosteronelevels,delayedpuberty,andcertaintypesofbreastcancerinwomen.However,non-medicaluse,
suchasforbodybuildingorperformanceenhancement,isoftenillegalandsubjecttoregulationsgoverningthe
useofcontrolledsubstances.

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Inthecontextofaquaculture,methyltestosteronemaybelegallyusedforsexreversalinfishspeciesliketilapiain
accordancewithrelevantregulationsandguidelines.It'sessentialforindividualsandbusinessesinvolvedinthe
productionanddistributionofmethyltestosteronetocomplywithapplicablelawsandregulationstoensurelegal
andethicalpractices.
Methyltestosteronepowderforsale-wheretobuypure17-MT
powder?
17a-methyltestosterone(MET)powderisoftenincorporatedintofishfeedtoinducemaledevelopmentinfemale
fish,whichhelpsinachievingmonosexpopulationsforcommercialproduction.Owingtotheeconomic
advantagesofculturingmaletilapia,leadtomoreandmoresupplierandmanufacturertobuy17MTpowderto
makeaquaculturepremix.Methyltestosteroneisasyntheticsteroidpowder,Wheretobuyhighquality17alpha

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methyltestosteronepowder,Aea.ltdwillbeoneofsteroidpowdermanufactureryoudon'twanttomiss.It's
essentialtocomplywithlocalregulationsandobtainmethyltestosteroneproductsfromreputablesourcesto
ensureproperandlegaluseinaquaculturepractices.
Reference:
[1]Nguyen,D.H.,Nguyen,N.T.H.,Nguyen,H.T.,Nguyen,N.V.,&Vo,T.V.(2023).“Sexreversalusing17α-methyltestosterone
immersionanditseffectonsexreversalandgrowthperformanceofNiletilapia(OreochromisniloticusL.,1758).”TheJournalof
AgricultureandDevelopment22(6),22-31.
[2]Alam,M.S.,Roy,A.,Shathi,S.B.,Deb,S.,Alam,M.S.,&Mondal,M.N.(2023).Doseoptimizationof17α-methyltestosteroneto
maximizesexreversalofNiletilapia(Oreochromisniloticus).ResearchinAgricultureLivestockandFisheries9(3),377-384.
[3]Duong,B.V.,&Pham,T.A.(2006).StudyontheeffectsoftemperatureandageonsexchangeresultsofOreochromisniloticus
tilapiabysoaking17α-methyltestosteronehormone(Unpublishedmaster’sthesis).VietnamAcademyofAgriculture,HaNoi,
Vietnam.
[4]Andersen,L.,Holbech,H.,Gessbo,A.,Norrgren,L.,&Petersen,G.I.(2003).Effectsofexposureto17alpha-ethinylestradiol

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duringearlydevelopmentonsexualdifferentiationandinductionofvitellogenininzebrafish(Daniorerio).ComparativeBiochemistry
andPhysiologyCToxicologyPharmacology134(3),365-374.https://doi.org/10.1016/s1532-0456(03)00006-1.
[5]El-Saidy,D.M.S.D.,&Gaber,M.M.A.(2005).Effectofdietaryproteinlevelsandfeedingratesongrowthperformance,
productiontraitsandbodycompositionofNiletilapia,Oreochromisniloticus(L.)culturedinconcretetanks.AquacultureResearch
36(2),163-171.https://doi.org/10.1111/j.1365-2109.2004.01201.x.
[6]El-Zaeem,S.Y.,&Salam,G.M.(2013).Productionofgeneticallymaletilapiathroughinterspecifichybridizationbetween
oreochromisniloticusandO.aureus.IranianJournalofFisheriesSciences12(4),802-812.
https://doi.org/20.1001.1.15622916.2013.12.4.8.7.
[7]El-Desokyel-SI,ReyadM,AfsahEM,DawidarAA."Synthesisandchemicalreactionsofthesteroidalhormone
17alpha-methyltestosterone."Steroids.2016Jan;105:68-95.doi:10.1016/j.steroids.2015.11.004.Epub2015Dec2.PMID:26639430
[8]LISSERH,ESCAMILLARF,GORDANGS."Dosageofmethyltestosterone."JClinEndocrinolMetab.1953Jun;13(6):732.doi:
10.1210/jcem-13-6-732.PMID:13061593
[9]OthmanR,RonXJ,YaoH,O'BryantP,RappD,PeiJC,WuHJ,WangHP."Theeffectofmethyltestosterone(MT)onsex
differentiationandgrowthinjuvenileyellowperch(Percaflavescens)."FishPhysiolBiochem.2022Feb;48(1):161-171.doi:
10.1007/s10695-021-01038-0.Epub2022Jan18.PMID:35039993