Ariandi-Presentasi Jurnal Penelitian Madu.pptx

Review Jurnal Penelitian Madu

1. PRESENTASI MATA KULIAH PROKARIOT SIMBION – MIK171G ARIANDI – G3601231006

2. PRESENTASI MATA KULIAH PROKARIOT SIMBION – MIK171G

3. The Australian stingless bee, Tetragonula carbonaria (Hymenoptera: Apidae), is found along the eastern coast of Australia from Queensland to New South Wales. It is one of the few Australian corbiculate (pollen basket) bee species to form large social colonies and plays a vital role in the pollination of native and commercially relevant plants Stingless beehives can be rescued from the environment when destructive actions such as tree clearing reveal their existence. In such cases, the hives can either remain in their existing cavity, for example, within a cut log or tree branch, or they may be transplanted into an artificial cavity within a specifically manufactured wooden box Bacteria residing in the guts of pollinating insects play a key role in nutrient acquisition, digestion, and resistance to pests and diseases. Imbalances in microbial flora in response to environmental change and stress can therefore impact insect health and resilience Gut microorganisms also contribute to the metabolism of lipids and proteins, the detoxification of plant secondary metabolites and pesticides, and the production of antimicrobial compounds. 3 Background This study is aimed at defining the core gut microbiome of T. carbonaria and exploring the impact of colony transplantation on gut health. Specifically, we wanted to know ( i ) whether gut microbiome taxonomic diversity differed between individual foragers from natural (log) and manufactured (box) hives and (ii) whether gut microbiome functional potential differed between individual foragers grouped by hive type. (Mills et al. 2023)

4. METHODS Sample Collection 16S rRNA Gene Pyrosequencing Bioinformatics Statistical Analyses (Mills et al. 2023)

5. Result & Discussion Fig. 1 Relative abundance of bacterial genera in the bee gut. Bar plot shows the relative abundance of ASVs greater than 1% found in the gut of 11 T. carbonaria individuals from two hive types: artiicial , “box” and natural, “log”. To account for diferences in sampling depth between samples, data were rareied prior to conversion to relative abundance. Colors indicate the summed relative abundance at the level of genus in an individual bee sample (Mills et al. 2023) Lactobacillus was the most dominant taxon overall (65% of all ASVs), Acetobacteraceae (25%) and Bombella (5.15%) were also abundant. Individual bees averaged 11 unique ASVs in their gut with the observed number of species ranging from 4 to 11 for all but one individual, box-3, which had 40 ASV

6. Result & Discussion Fig. 2 Relative abundance and prevalence of ASVs in the T. carbonaria core gut microbiome. Core microbiome was deined as ASVs identiied in six or more of the eleven T. carbonaria individuals. Relative abundance refers to the average abundance of a bacterial amplicon sequence variant (ASV) across all bees. Prevalence refers to the total number of individual bees from which the ASV was identiied divided by eleven (and expressed as a percentage). For example, a prevalence of 100% indicates that an ASV was identiied in eleven out of eleven bees; a prevalence of 50% indicates that an ASV was identiied in six out of eleven bees (Mills et al. 2023) The core T. carbonaria gut microbiome, defined as ASVs present in at least 50% of the individuals, consisted of five unique ASVs from three taxonomic classifications

7. Result & Discussion (Mills et al. 2023) Table 1 Core ASVs identified with BLAST search

8. Result & Discussion (Mills et al. 2023) Table 2 Difference between bee gut microbiomes grouped by hive type

9. Result & Discussion ( Tang et al . 2021) Fig. 3 Top 30 functional pathways present in all individual bee gut microbiomes. Functional pathways inferred from 16S rRNA gene amplicon sequencing indicates over 122 pathways present in 80% of individual bees (full list in Table S5). Heat map displays the top 30 functional pathways present in all individual bees (Mills et al. 2023)

10. PRESENTASI MATA KULIAH PROKARIOT SIMBION – MIK171G conclusion This study has shown that T. carbonaria hosts a core microbiome of functionally important bacteria, including Lactobacillus spp. and Acetobacteraceae spp. which are important for nutrient acquisition, defense, and host homeostasis. Our inferred functional pathway analysis suggests that while hive transplantation impacts the whole microbiome, the core microbiome (and its functional potential) is preserved. These results provide a positive outlook for the Australian bee-keeping industry, which is heavily reliant on hive splitting and transplantation for the preservation and propagation of colonies (Mills et al. 2023)

11. TERIMA KASIH PRESENTASI MATA KULIAH PROKARIOT SIMBION – MIK171G REFERENSI: Mills TJT, Nelson TM, Pearson LA, Neilan BA. 2023. Hive Transplantation Has Minimal Impact on the Core Gut Microbiome of the Australian Stingless Bee, Tetragonula carbonaria . Microb Ecol . 86(3):2086–2096. doi:10.1007/s00248-023-02222-w.