Assay Development and Drug Repurposing Core

Tom Dexheimer, Ph.D.
Manager, MSU Assay Development and Drug Repurposing Core
Michigan State University
Drug Discovery Interdisciplinary Event May 14, 2015

1. Assay Development and Drug Repurposing Core Drug Discovery Forum Tom Dexheimer, Ph.D. Manager, MSU Assay Development and Drug Repurposing Core May 14, 2015

2. MSU Assay Development and Drug Repurposing Core To provide equipment and expertise for high-throughput screening techniques to MSU r esearchers To provide MSU researchers with access to cost-effective chemical library screening for identification of molecules to further interrogate particular biological processes or potential leads for drug development (or drug repurposing ) To promote a training environment that will expose students to the techniques of high-throughput assay design, development, and HTS screen execution To provide assistance and/or the generation of preliminary data for grant/funding opportunities Goals of the ADDRC

3. E quipment – Liquid handling Multidrop 384 Reagent Dispenser Peristaltic pump based cassette dispenser Dispense 96- and 384-well plates 5 to 395 m L volume range (5 m L increments) EL406 Microplate Washer Dispenser Peristaltic pump based cassette dispenser 1 m L and 5 m L cassettes 96-well washer module S yringe pumps dispensers Variable dispense and aspiration rates Biomek FX Laboratory Automation Workstation Automated/programmable robotic dual arm system with 96-multichannel pipettor and Span-8 pipettor (8 individually controlled channels ) Equipped with 384-pin tool Small volume plate to plate transfers (i.e . compound plate to assay plate) Volume range - 100 nL to 200 nL

4. Cytation 3 Cell Imaging Reader Equipment – Readers Automated digital microscopy Objectives: 4x and 20x (currently available) Brightfield, 365 nm, 465 nm, 590 nm Temperature and CO 2 /O 2 control Synergy Neo Multi-mode Reader Combines filter-based optical system and monochromator optics, providing both excellent performance and assay versatility Detection modes: Fluorescence , time-resolved fluorescence , fluorescence polarization , Alpha, luminescence , UV-visible absorbance Multi-plate stacker (30 plates ) Functional Drug Screening System FDSS/µCELL Kinetic plate reader with an integrated dispensing head and imaging-based detector High-speed digital CCD camera 0.5 s to 100 s/data point 480 nm / 540 nm 96- or 384-well compatible Ca 2+ , cAMP , membrane potential, etc.

5. Chemical L ibraries and Informatics GREENScreen Integrated compound management and high throughput screening data storage and analysis system Chemical Libraries 6571 compounds

6. MSU Drug Discovery Drug Discovery listserv ( [email protected] ) Bring together researchers from all disciplines at MSU who are interested or involved in drug discovery and development Drug Discovery Seminar Series Fridays from 9:00 to 10:00 AM in B448 Life Science Building http:// phmtox.msu.edu/core-facilities/Assay/DDIG.htm Michigan Drug Discovery Symposium SAVE THE DATE September 9 th -10 th Contact Information Tom Dexheimer , Ph.D. B411 Life Science Building Email: [email protected] Office: 517-884-4998 *The ADDRC facility was established through the generous support of the Vice President for Research and Graduate Studies, the Pharmacology and Toxicology Department, CNS, COM, CHM, CVM, and AgBioResearch .