Assement CNSL_Liver_Injury_Presentation.pptx

CNSL Liver injury

1. Assessment of Oxidative Liver Injury Caused by CNSL Simple Overview with Medical Theme

2. Introduction Cashew Nut Shell Liquid (CNSL) is an industrial byproduct containing toxic compounds like cardanol and cardol. This study examined how topical exposure to CNSL affects liver and kidney tissues in male Wistar rats.

3. Experimental Design • 3 Groups of Wistar rats: Control, CNSL-treated, and Sunflower oil (SFO) control. • 60 µL of CNSL or SFO applied to depilated skin daily for 45 days. • Body weight, organ weight, blood, and tissue samples collected for analysis.

4. Key Findings - Physical Changes • CNSL caused significant body weight loss and increased liver weight. • Visible skin damage including inflammation, burns, and hair loss.

5. Biochemical Effects • Increased liver enzymes (SGPT, SGOT) indicating liver injury. • Higher urea and lower creatinine levels suggest kidney stress. • Decreased antioxidant enzymes (SOD, catalase) → higher oxidative stress.

6. Inflammatory and Genetic Changes • Elevated inflammatory markers (TNF-α, IL-6). • Increased p53 and Akt expression but reduced Bcl-2 expression. • Indicates apoptosis (programmed cell death) activation.

7. Histopathology • Liver: Central vein and sinusoidal dilation, infiltration of blood cells. • Kidney: Disrupted Bowman’s capsule and tubular degeneration.

8. Molecular Docking • Cardanol and cardol interact with Bcl2 protein domains (BH1, BH4). • These interactions may inhibit anti-apoptotic functions of Bcl2.

9. Conclusion • CNSL exposure causes oxidative stress, inflammation, liver and kidney damage, and apoptosis. • Protective measures are essential for workers in cashew processing units.