atomic absorption spectrophotometry (sample preparation)

this slide will help you to learn about the sample preparation for #AAS
and also shows a demonstration of how sample run in #AAS

1. Analytical chemistry Arooj Gohar Roll#26

2. Atomic absorption spectrophotometry Sample preparation

3. Sample preparation: Sample preparation with flame method can often be kept to a minimum as long as chemical or spectral interference are absent essentially all that is required is to obtain a sample in the form of diluted and filtered solution.

4. Forms of analyte : i t often makes no difference what chemical form of analyte is because it will dissociated to the free element vapor in the flame thus several elements in the blood, urine, cerebral spinal fluid and biological fluids by direct aspiration of the sample .

5. Sample dilution : U sually dilution with water will be required to prevent clogging of the burner.

6. Burner:

7. Matrix selection: In The preparation of standards, the matrix of the analyte must always be matched. For example : If lead in gasoline is to determined, a stimulated Solvent matrix must be used for standards, not water.

8. Chemical interference: Chemical interferences can be overcome by simple addition of a suitable reagent solution. Serum is diluted with a solution containing EDTA for the determination of calcium in order to prevent interference from phosphate. Sodium and potassium in concentrations equal to those in serum are added to calcium standards to prevent ionization interference.

9. Applications of atomic absorption spectrophotometry to biological samples : This technique is widely used for metal analysis in biological fluids and tissues in environmental samples such as air and water and in occupational health and safety areas. Routine applications of flame emission spectrophotometry to biological samples are generally limited to alkali and alkaline earth metals

10. Replacement of flame emission measurements: Ion selective electrode measurements have largely replaced the flame emission measurements in the clinical chemical laboratory. As ion selective electrode measure the activity of ion directly Rather than the concentrations.

11. Overview of atomic absorption spectroscopy:

12. Thank you For listening