Genus of usually motile Oval or spherical bacteria Thick-walled cysts May produce large quantities of capsular slime. Azotobacter species cells : stained with Heidenhain's iron hematoxylin, ×1000 Azotobacter
Description Genus of free-living diastrophic bacteria whose resting stage is a cyst . It is primarily found in neutral to alkaline soils , Aquatic environments, and on some plants . IMAGE : Azotobacter cyst.
Significance It has several metabolic capabilities : including atmospheric nitrogen fixation by conversion to ammonia. Their unique system of three distinct nitrogenase enzymes makes these bacteria of particular interest to scientists, who may work toward a better understanding of nitrogen fixation and its role in agriculture. Azotobacter spp: the highest metabolic rate of any organisms.
Advantages of Azotobacter: Heaviest breathing organism and requires a large amount of organic carbon for its growth. Competitor for nutrients in soil Growth promoting substances Fungistatic substances.
Nitrogen fixation Azotobacter species are free-living, nitrogen-fixing bacteria; in contrast to Rhizobium species, They normally fix molecular nitrogen from the atmosphere without symbiotic relations with plants, some Azotobacter species are associated with plants . Nitrogen fixation is inhibited in the presence of available nitrogen sources, such as ammonium ions and nitrates .
Nitrogen fixation Azotobacter species have a full range of enzymes needed to perform the nitrogen fixation: ferredoxin, hydrogenase, important enzyme nitrogenase . The process of nitrogen fixation requires an influx of energy in the form of adenosine triphosphate