bakteri pada kulit dan jaringan tubuh .pptx

Infeksi pada kulit

1. Sitti hadijah BAKTERIOLOGI INFEKSI KULIT & JARINGAN LUNAK

2. Staphylococcus spp Mycobacterium leprae PENDAHULUAN

3. Staphylococcus : S. aureus S. epidermidis S. saphrophyticus Mycobacterium leprae : slide only Uji Katalase Uji Koagulase Mannitol Salt Agar (MSA) Novobiocin test MRSA Identification identifikasi

4. Uji yg dilakukan Uji Katalase Uji Koagulase Mannitol Salt Agar (MSA) Novobiocin test

5. Staphylococcus spp

6. The c a ta l ase te s t is pr i marily u se d to distinguish among Gram-positive cocci Staphylococcus are catalase-positive, m e mbers of the genera St r epto c oc c us a nd Enterococcus are catalase-negative. Katalase test

7. Metode : slide (drop) method tube method tube (slant) method Catalase 2H 2 O 2  O 2 + 2H 2 O Streptococci vs. Staphylococci

8. Catalase POS Staphylococcus Catalase NEG

9. Tes katalase metode tabung

10. differentiates strains of Staphylococcus aureus from S . epidermidis and other coagulase- negative species S. aureus strains are usually capable of coagulating EDTA-treated plasma in the tube test and will produce clumps of cells in the slide test. Tes koagulase

11. method : Slide Test Tube Test Metode tes koagulase

12. Coagulase Test (Slide Test)

13. S. aureus Coagulase Fibrinogen  Fibrin Differential Ch a racteristics

14. Coagulase NEG Coagulase POS Staphylococcus aureus

15. Contains : Mannitol Phenol Red as a pH indicator (pH = 7.8) Reaction Mannitol Fermented  acid produced pH < 6.9 : yellow color pH > 8.4 : red color Mannitol salt agar

16. various spesies on T r i p t i c a s e S o y A ga r ( T SA ) A. S. aureus B. S. epidermidis C. E. coli

17. various spesies on T r i p t i c a s e S o y A ga r ( T SA ) A. S. aureus B. S. epidermidis C. E. coli

18. Differentiate between S. epidermidis w/ S. saprophyticus Using Novobiocin disc ( 5  g)  < 15 mm = Resisten  S. saprophyticus Novobiocin test

19. MRSA : Methicillin-Resistant Staphylococcus aureus Methicillin  Oxacillin  Cefoxitin CLSI M100 Using Cefoxitin disc :   21 mm : MRSA  > 21 mm : non - MRSA Mrsa

20. S u s pe c t M R S A

21. Collect Sample from wound

22. Pengambilan sampel mlalui swab hidung

23. Sample : Skin scrap using scalpel Using Slide with Ziehl-Neelsen Stain method Presence of globi (singular = globus): encapsulated amphophilic masses of non- viable bacilli found within macrophages or in extracellular space Mycobacterium leprae

24. Result : (Bacterial Index) 1+ At least 1 bacillus in every 100 fields. 2+ At least 1 bacillus in every 10 fields. 3+ At least 1 bacillus in every field. 4+ At least 10 bacilli in every field. 5+ At least 100 bacilli in every field. 6+ At least 1000 bacilli in every field.

25. O n H an d Pengamatan & Pelaporan Preparat Demo Uji Katalase Kultur Media dari spesimen langsung (amies swab) dengan : Blood Agar Plate McConkey Agar