Banana dna lab isolating dna from a banana activity

1. Isolating DNA from a
banana…yes a banana!!!

3. How do we take out the DNA?
1.a) Salt
Helps DNA keep together during the
process.

4. How do we take out the DNA?
1.b) Detergent (shampoo)
Breaks down the cell membrane
and nuclear membrane.
(NO BUBBLES!!)

5. Filter
2. Filter the banana “goo” with a coffee filter.
-This removes everything that you do not
need.
-Take the filtrate.
Banana goo
What you need!!!

6. "Clean" the DNA
Use a meat tenderizer to digest any proteins
associated with the DNA.

7. Now we remove the DNA
- Add iced rubbing alcohol.
- This will allow the DNA to
precipitate.
- Now you may remove the
DNA with a toothpick.

8. Instructions and Measurements
Materials: (per group)
•piece fruit (~1/4 of a banana)
•1 coffee filter
•two cups (or beakers ~100 mL)
•15 ml of iced alcohol
•~1.5 g (1-2 mL) table salt (NaCl)
•10 mL water
•30 mL liquid soap
•1 toothpick