Bangi Tissue Culture Lab - Production capacity-150421.pptx

PRESENTATION

1. MIE TISSUE CULTURE LAB Project Plan BY: DR. MUHAMAD RADZALI MISPAN 19th APRIL 2021 21 April 2021 Private & Confidential 1

2. Content Bangi Tissue Culture Lab : Production capacity Project plan 21 April 2021 2 Private & Confidential

3. PLANT TISSUE CULTURE Steps & Process 21 April 2021 3 Private & Confidential

4. STEPS OF CULTURING PROCESS 1 MONTH 4-5 MONTHS 1 MONTHS 1 MONTH 1 MONTH Process in the lab Outside the lab Clonal seedlings are; free from disease have uniform growth can produce early yield as compared to sucker planting material

5. SUBCULTURE ELONGATION ROOTING INITIATION *Assumption: 1 Jar, 4 explants MULTIPLICATION RATE OF BANANA EXPLANTS

6. FLORA VITRO, ANM Production Capacity 21 April 2021 6 Private & Confidential

7. 21 April 2021 7 Private & Confidential 1 rack: 5 Level 1 level : 7 tray 1 tray: 20 jar 1 jar : 4 explants 20 Jar x 7 trays x 5 level = 700 jar 700 jar x 4 explants =2800 explants 2800 explants x 18 racks = 50,400 explants INCUBATION ROOM 1 – 18 Racks

8. 21 April 2021 8 Private & Confidential 1 rack: 5 Level 1 level : 7 tray 1 tray: 20 jar 1 jar : 4 explants 20 Jar x 7 trays x 5 level = 700 jar 700 jar x 4 explants =2800 expants 2800 explants x 12 explants = 33,600 explants INCUBATION ROOM 2 – 12 Racks Total production capacity : ± 84,000 EXPLANTS ( INCUBATION 1 & 2 )

9. TISSUE CULTURE Target production 21 April 2021 9 Private & Confidential

10. PRODUCTION TIMELINE Agreement still under evaluation with Legal Advisor of MOSTI Expected for lab preparation to commence in May 2021 Production will start in June 2021 Estimation of seedlings production is based on the maximum capacity at one time at ANM Period for every production cycle is 8 months ( 6 months in the lab + 2 months at the nursery) 21 April 2021 10 Private & Confidential

11. ESTIMATION OF SEEDLINGS PRODUCTION 2021-2022     BUDGET 2021 BUDGET 2022 PLANT   TOTAL SEEDLINGS/CYCLE TOTAL CYCLE TOTAL SEEDLINGS/MONTH TOTAL SEEDLINGS/CYCLE TOTAL CYCLE TOTAL SEEDLINGS/MONTH     unit unit unit unit unit unit PLANT SEEDLINGS               Banana                               Jan         15,000 1 15,000 Feb         15,000 2 30,000 March         15,000 2 30,000 April         15,000 2 30,000 May         15,000 2 30,000 June         15,000 2 30,000 July         15,000 2 30,000 August         15,000 2 30,000 September         15,000 2 30,000 October         15,000 2 30,000 November         15,000 2 30,000 Disember         15,000 2 30,000                 TOTAL   -   - - 23 345,000 21 April 2021 11 Private & Confidential

12. PRODUCTION TIMELINE 2021-2022 CALCULATION OF EXPLANTS 21 April 2021 12 Private & Confidential   Lab preparation 1 month   Selection of mother plant / sampling / collecting 1 week   Initiation 1 month   Subculture / multiplication 1 cycle - 3 weeks   Elongation & Rooting 1 month   Acclimatization 1 month   Hardening 1 month 2021 2022

13. TOTAL EXPLANTS PER MONTH IN THE LAB & PLANTLETS AT NURSERY 2021 LAB NURSERY Culturing process Acclimatization Hardening May - June 32 July 133 August 676 September 5522 October 34,847 November 68,276 Disember 76,463 24,576 21 April 2021 13 Private & Confidential

14. 2022 LAB NURSERY Culturing process Acclimatization Hardening Jan 71,001 32,768 24,576 Feb 77,824 32,768 32,768 March 73,388 28,672 32,768 April 68,267 36,846 32,768 May 63,810 32,768 36,846 June 76,463 32,768 32,768 July 76,459 32,768 32,768 August 76,459 32,768 32,768 September 76,459 32,768 32,768 October 76,459 32,768 32,768 November 78,849 28,672 36,846 Disember 76,613 32,768 28,672 21 April 2021 14 Private & Confidential TOTAL EXPLANTS PER MONTH IN THE LAB & PLANTLETS AT NURSERY

15. TISSUE CULTURE Income vs Expenditure 21 April 2021 15 Private & Confidential

16. TISSUE CULTURE LAB FINANCIALS ESTIMATION INCOME VS EXPENDITURE ON 2021-2022 16 2021 2022 Headcount @ Total 13 13 CAPEX RM 282,000 INVENTORIES RM 50,087 Estimation Income/Year - RM897,000 Total OPEX ( Operation – Chemicals in the lab, nursery, salaries, Lab & Nursery rental, Training, Consumable items, etc) RM 425,448 RM 825,264 Profit vs (Loss) (RM 425,448) RM 71,736 NOTE: - Estimation price per banana plant seedlings : RM 2.50 – RM 2.60 - Price change depends on the quantity of plant seedlings purchased & current market Achievement of target depends on sample quality, complete protocol & methodology & sufficient manpower

17. CAPEX ESTIMATION – LAB FACILITIES 21 April 2021 17 Private & Confidential

18. 21 April 2021 18 Private & Confidential No Asset placement Equipment/Machineries Quantity Price/Unit (RM) Total (RM) Justification 1 Inoculation room Infrared Micro-Sterilizer 6 1,500 9,000   To sterilize metal inoculating lopps ; scalpel, blade etc without using an open flame 2 Medium room Magnetic stirrer with hotplate (multi-type) 1 7,000 7,000   To quickly stirring or mixing a solution 3 pH meter 1 2,000 2,000   To determine the acidity or basicity of aqueous solutions 4 Autoclave Room Autoclave Machines 2 36,000 72,000   To sterilize equipment, instruments, medium and also infectious waste 5 R&D Room Microscope with camera 1 6,000 6,000   To study the external features & examine structures of plant samples 6 Washing Room (Dry) Drying Oven with Fan 1 5,000 5,000   To dry the apparatus or plant samples as quickly as possible TOTAL (RM) 101,000 N o Construction Details Quantity Price/Unit (RM) Total (RM) Justification Justification 1 Nursery Nursery structure & Store L/SUM 50,000 50,000   Acclimatized and hardened the tissue culture plant seedlings   2 Laboratory Repair structure – roof, etc L/SUM 5,000 5,000 Repair roof structure of lab building   TOTAL (RM)       55,000     A) MAIN BUILDING B) LABORATORY No Aset placement Equipment/Machineries Quantity Price/Unit (RM) Total (RM) Justification 1 Nursery Lorry (1 ton) 1 10,000 10,000   To transported the plant seedlings to customers 2 Toyota Hilux 1 90,000 90,000   To sampling the plant materials TOTAL (RM) 100,000 C) TRANSPORTATION

19. 21 April 2021 19 Private & Confidential D) NURSERY No Aset placement Details Quantity Price/Unit (RM) Total (RM) Justification 1 Nursery Mixer (Penggaul substrate) 2 1,500 3,000   To mix the substrates (Sand, soil, chicken dung)   TOTAL (RM)       3,000     E) IT EQUIPMENT No Aset placement Details Quantity Price/Unit (RM) Total (RM) Justification 1 Office Computer - dekstop 1 3,000 3,000   Administration, preparation of word documents such as reports, processing of work documents such as work orders/financial reports 2 Laptop 5 3,000 15,000   Senior Exec (2), Junior Lab Exec (1), Project Exec cum Admin (1), Agronomist (1) 3 Photostat Machine + Printing 1 5,000 5,000   Produce paper copies of a documents, print important documents, such as accounting documents, employee information, report etc   TOTAL (RM)       23,000     GRAND TOTAL: RM 282,000

20. INVENTORIES ESTIMATION 21 April 2021 20 Private & Confidential

21. 21 April 2021 21 Private & Confidential             INVENTORIES UNIT RM/UNIT   BUDGET JUSTIFICATION               A B   C           (A X B )           RM   A) LABORATORY           LAB TOOLS/EQUIPMENT           Glass Jar 20,000 1.0   20,000 To place the culture explants Stainless steel, scalpel holder no. 3 10 27   270 To hold the scalpel Scissors/steel 150mm sharp 10 20   200 To cut the tissue of explants Forceps sharp end (15cm) stainless steel 10 30   300 Culturing process Forceps sharp end (250mm) stainless steel 10 30   300 Culturing process Forceps blunt end (15cm) stainless steel 10 30   300 Culturing process Forceps blunt end (250mm) stainless steel 10 30   300 Culturing process Aspirator bottle plastic 35 L 3 200   600 Storage of distilled water Aspirator bottle plastic 25 L 3 200   600 Storage of distilled water Pipette (20-200 μ l) 2 550   1,100 Medium preparation Single channel adjustable volume pipette 1 - 10 ml 2 750   1,500 Medium preparation Pipette tips (20-200 μ l) 1 170   170 Medium preparation Pipette tips (1-10ml) 1 130   130 Medium preparation Magnetic stirrer bar 20 8   160 Homogenize the solution Erlenmeyer flask, narrow neck with graduation, 150ml 20 8   160 To place/store the solution Erlenmeyer flask, narrow neck with graduation, 250ml         To place/store the solution Scalpel blade to fit blade No 10, 11,12, 15 10 18   180 Culturing orocess Wash bottle 500ml 4 50   200 Medium preparation Spatula, stainless steel spoon flat end (150 mm) 10 18   180 Medium preparation Schott Duran Laboratory bottle (2000 ml) 50 40   2,000 Medium preparation Schott Duran Laboratory bottle (1000 ml) 30 30   900 Medium preparation Schott Duran Laboratory bottle (500 ml) 10 22   220 Medium preparation Schott Duran Laboratory bottle (250 ml) 10 18   180 Medium preparation Schott Duran Laboratory bottle (100 ml) 10 16   160 Medium preparation Petri dish disposable 90mm x 15mm 4 180   720 Culturing process Beaker glass 1L 10 30   300 Medium preparation Beaker glass 500 ml 5 19   95 Medium preparation Beaker glass 250 ml 5 15   75 Medium preparation Beaker glass 100 ml 5 12   60 Medium preparation Dropping bottles with Dropping Pipettes 10 10   100 To place/store the solution Magnetic retriever 2 30   60 Medium preparation

22. 21 April 2021 22 Private & Confidential Floor mounted stand 2 259   518 To place the tissue paper wipe jumbo Graduated beakers 2000 ml 15 50   750 Medium preparation Graduated beakers 1000 ml 10 30   300 Medium preparation Graduated measuring cylinder 500 ml 1 40   40 Medium preparation Graduated measuring cylinder 250 ml 1 35   35 Medium preparation Graduated measuring cylinder 100 ml 5 25   125 Medium preparation Graduated measuring cylinder 50 ml 3 20   60 Medium preparation Carousel stand for up 1 200   200 To place the pipettes Diamond weighing boat 100 ml 1 373   373 To place the chemicals when measuring weight Diamond weighing boat 30 ml 1 215   215 To place the chemicals when measuring weight Measuring cylinder 1L 5 56   280 Measuring liquid - chemicals, medium Test tube 1 120   120 To place the explants for rooting purposes Test tube rack 1 30   30 To place the test tube Falcon tube 1 330   330 To place the samples (plant tissue) Falcon tube rack 2 30   60 To place the falcon tube Syringe 1 75   75 To insert the liquid hormone into PTFE filter sterilization Cork Borrer 1 56   56 Use for makin holes in cork / samples Bio Hazard/Autoclave Bag (12 x 16') 2 50   100 To place the apparatus/glass jarin autoclave beg when sterilization Bio Hazard/Autoclave Bag (20 x 30') 1 120   120 To place the apparatus/glass jarin autoclave beg when sterilization PTFE filter strelization 1 110   110 To sterilize the liquid hormone Biohazard Disposal Container 4 100   400 To remove residual medium and contamination Biohazard Sharp waste bin , 20L 4 35   140 To remove sharp tools such as blade Lab Coat 10 60   600 Attire for lab technician in the lab Metal sheet 10 5   50 Culturing process FURNITURE (LAB)           Lab chairs 6 15   90 Use by laboratory staff during culture of explants             OTHERS           Washing Machine (Manual) 1 500   500 Easily wash the lid of glass jar in large scale / labcoat and other materials Microwave 1 250   250 To dilute the solution UV Lamp 6 180   1,080 To sterilize the interior and contents in laminar air flow before usage to prevent contamination of the experiment

23. 21 April 2021 23 Private & Confidential B) OFFICE           FURNITURE & EQUIPMENTS                       Office tables (Officer) 4 300   1,200 Use by laboratory staff at office Office tables (technician) 6 150   900 Use by laboratory staff at office Office chairs 10 150   1,500 Use by laboratory staff at office Whiteboard 2 300   600 Office requirement Laminating Machine 1 180   180 Office requirement             INVENTORIES UNIT RM/UNIT   BUDGET JUSTIFICATION               A B   C           (A X B )           RM   C)NURSERY           EQUIPMENT           Industrial Trays 100 50   5,000 To place the jar/plant seedlings (in polibeg) Trolley Hand Truck 5 150   750 Lifting/moving plants seedlings/substrates, etc Basin 10 50   500 To place the plant substrate Whiteboard 1 250   250 For recording activities and work plan at the nursery Chairs 4 15   60 Use by general workers during deflaksing process Shovel 5 5   25 Manual mixing of plant substrates Sieve 3 15   45 To filter a medium gel before transfer the plantlets in the tray Plastic tables 3 100   300 Use by general workers during deflaksing process Gardening gloves 10 4   40 Use by general workers during deflaksing and transfer plants into polibeg MANURING           Knapsack sprayer 5L 1 80   80 Manuring Knapsack sprayer 10L 1 130   130 Manuring GRAND TOTAL: RM 50,087

24. MARKETING Strategy 21 April 2021 24 Private & Confidential

25. Strategy April – May : To start compiling and establishing network with possible clients To start culturing work in June 2021 To focus on 3 marketable and high-demand banana varieties – Pisang Berangan , Pisang Nangka dan Pisang Tanduk Pre-book system (3 months before the seedlings are ready) Online presence ( whatsapp , facebook , Live stream, Instagram, twitter etc ) Possible clients Agropreneur (Large scale & small scale) Government agencies (networking with FAMA, MARDI, Jabatan Pertanian negeri etc ) Exhibition Online platform – through website, Shopee 21 April 2021 25 Private & Confidential

26. Other products Forest tree – Kelampaian , Albizia , Eucalyptus Bamboo Coconut Ornamental plants – Orchird , Money Plants, Palms Green Souvenir 21 April 2021 26 Private & Confidential