Basic Difference Between HPLC Vs GC Used In API Pharma Companies

Basic Difference Between HPLC Vs GC Used In API Pharma Companies:
Methods, Application,

1. HPLC Vs GC

2. Difference: All Copyrights Reserved At API Pharmaprenuer Tech S.No . Parameter HPLC GC Methods 1 Mobile Phase Liquid Inert Gas   Separation Techniques Based On Polarity Based on volatality   Compound Properties Soluble (Volatile or non volatile) Volatile   Working Temperature RT (20-25°C) High Temp. (150-300°C)   Speed Slower Faster   Resolution Low Resolution for polarity Low Resolution for molecular Weight

3. Difference: All Copyrights Reserved At API Pharmaprenuer Tech Equipments     Column Length Short & wide (Length 30cm & internal Dia. 8mm) Long & thin ( Lenght : 100 m & Internal Dia : 0.1-1 mm)   Solvent Must be polar Must be vaporize   Detection Non Detective like Ultraviolet-Visible spectroscopy or Refractive Index Detector Destructive like a flame ionization detector (FID) which is effective for analysing hydrocarbons. Or a thermal conductivity detector (TCD) can be used as it is a universal detection method for GC analysis   Cost Higher Lower

4. Difference: All Copyrights Reserved At API Pharmaprenuer Tech Application     Industries Inorganic ions, polymers, sugars , nucleotides, vitamins, peptides, proteins, lipids and tetracyclines , Basically Pharmaceutical Companies Oils, plant pigments, pesticides, fatty acids, toxins, air samples and Drugs (Intermediates).

5. Difference: Lab Demo Calculation: Silica Crucible: 35.93 gm Drug Wt: 2 gm Burnt Drug + Crucible Wt: 36.08 gm Wt of Ash: 36.08-35.93 = 0.15 gm % of ash in 2 gm drug = 0.15 / 2 *100 = 7.5% All Copyrights Reserved At API Pharmaprenuer Tech

6. Difference: All Copyrights Reserved At API Pharmaprenuer Tech Title Water Content Loss On Drying Method Involves Chemical Reaction Drying of Sample under specific Temp. at std duration Depends on Quality of reagent used Temperature Moisture content Humidity Bulk density Resistance to Airflow Formula = Mass of water in sample  /  mass of dry sample  *  100 = Mass of water in sample  /  Mass of wet sample  *  100

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