Basic information of s1 nuclease

The S1 nuclease was extracted from Aspergill suoryzae. The S1 nuclease is a specific
single-stranded endonuclease. It can degrade single-stranded DNA and
single-stranded RNA to produce 5'-single-stranded nucleotides or oligonucleotides.

1. Basic Information of S1 Nuclease
Source
The S1 nuclease was extracted from Aspergill suoryzae. The S1 nuclease is a specific
single-stranded endonuclease. It can degrade single-stranded DNA and
single-stranded RNA to produce 5'-single-stranded nucleotides or oligonucleotides.
Double-stranded DNA, and DNA-RNA hybrid molecules are more resistant to S1
nucleases, and only high concentrations of enzymes allow them to be digested. It
hydrolyzes single-stranded DNA at a rate that is 75 000 times faster than hydrolyzed
double-stranded DNA. The optimum pH for the enzymatic reaction was 4.0 to 4.3,
and the enzyme activity decreased by 50% at pH 4.9.
The S1 nuclease
is derived from Aspergillus oryzae and is a highly single-strand specific endonuclease
that degrades single-stranded DNA or RNA under optimal conditions of enzymatic re
action to produce 5'-phosphate. Single nucleotide or oligonucleotide.

Structural and Catalytic Properties of S1 Nuclease
It is relatively insensitive to double-stranded DNA, double-stranded RNA, and
DNA-RNA hybrids. The rate of hydrolysis of single-stranded DNA is typically 75,000
times faster than hydrolysis of double-stranded DNA. This enzyme requires low
levels of Zn2+ activation with an optimum pH range of 4.0 to 4.3. Some chelating
agents (such as EDTA and citric acid) can strongly inhibit S1 nuclease activity.
In addition, phosphate buffer and 0.6% SDS solution can inhibit its activity, but it is

2. stable to urea and formamide of. The single-strand hydrolysis function of the S1
nuclease can act on a single-stranded region of a double-stranded nucleic acid
molecule and cleave the nucleic acid molecule from a single-stranded portion, and
such single-stranded region can be as small as one base pair. Because of this function,
the S1 nuclease analyzes the structure of the nucleic acid hybrid molecule
(RNA-DNA), localizes the RNA molecule, determines the position of the spacer
sequence in the eukaryotic gene, removes the prominent single-stranded tail in the
DNA fragment, and Opening the hairpin loop formed during double-stranded cDNA
synthesis works.
Principles of S1 nuclease localization
It is a method of determining the position of the 5' end of mIlNA in the corresponding
template soil. The mRNA is first hybridized with the a:p-labeled template, digested w
ith a single-strand specific Siase, and a single-stranded portion at both ends is remove
d, and then the P-labeled DLA-R}IA hybrid molecule is reused for determination D.
The method of 'VA sequence determines the template D} IA sequence. And electroph
oresis on the same gel plate as the former. The position of the DN A-RNA hybridizati
on band was observed by autoradiography to see which nucleotide position of the Dy
A nucleoside [1] acid ladder was equivalent, and the nucleotide type of the rnI2'V A t
erminal was known.
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