BENEFITS AND APPLICATIONS � OF � PLANT GROWTH PROMOTING � MICROBIAL TECHNOLOGIES.pptx

This presentation has focused on PGPR can be found in the rhizosphere. They live in association with roots and stimulate the plant growth and/or reduce the incidence of plant disease. Among the numerous PGP bacteria and fungi described up to now, the bacteria Azospirillum, Bacillus, and Pseudomonas, and the fungus Glomus are the genera more frequently mentioned on research reports. The important role that PGPR play in agriculture can be clearly deduced from the extensive research published until now.

1. BENEFITS AND APPLICATIONS OF PLANT GROWTH PROMOTING MICROBIAL TECHNOLOGIES Dr. Puja Pandey Assistant Professor Department of Plant Pathology B. A. college of Agriculture Anand Agricultural University, Anand, Gujarat

2. INTRODUCTION

3. Pérez-Montano et al ., 2014 Mechanisms

4. Trivedi et al ., 2016

5. Pérez-Montano et al ., 2014

6. Pérez-Montano et al ., 2014

7. Growth of bacterial colonies on NA media

8. Growth of bacterial colonies on NA media

9. Plates viewed in UV Transilluminator

10. Microscopic image of Pseudomonas spp

11. Alteration in defense related enzymes and compounds by abiotic and biotic elicitors in pea against downy mildew pathogen using

12. List of elicitors alone and in combination used in experiment. Treatments Name (source) Concentration T1 Salicylic acid (Sigma-Aldrich) 0.72 mM T2 Isonicotinic acid (Sigma-Aldrich) 0.5 mM T3 Oxalic acid (Sigma-Aldrich) 0.5 mM T4 Chitosan (Sigma-Aldrich) 1g/100ml (1%) T5 Pant Bioagent 1 (Pantnagar Biocontrol Laboratory) 10g/litre T6 Pant Bioagent 2( Pantnagar Biocontrol Laboratory) 10g/litre T7 Chitosan + Pant Bioagent 1 0.5g/100ml+ 5g/litre T8 Chitosan + Pant Bioagent 2 0.5g/100ml+ 5g/litre T9 Water - 16

13. Phytotoxicity test of chemical elicitors (Seed treatment) Chemical Concentration 0.5 mM 0.75mM 1mM 1.5mM Salicylic acid - - + + Isonicotinic acid - + + + Oxalic acid - + + + + sign indicates the phytotoxicity - sign indicates non-phytotoxicity 17

14. Fig. Effect of elicitors (seed treatment + foliar application) on Phenylalanine ammonia lyase (PAL) activity in pea under controlled condition 18

15. Fig. Effect of elicitors (seed treatment + foliar application) on Polyphenol oxidase (PPO) activity in pea under controlled condition 19

16. Fig. Effect of elicitors (seed treatment + foliar application) on Peroxidase (PO) activity in pea under controlled condition 20

17. Fig. Effect of elicitors (seed treatment + foliar application) on total phenol activity in pea under controlled condition 21

18. 22 Fig. Effect of elicitors (foliar application) on Phenylalanine ammonia lyase (PAL) activity in pea under controlled condition

19. Fig. Effect of elicitors (foliar application) on Phenylalanine ammonia lyase (PAL) activity in pea under controlled condition 23

20. Fig. Effect of elicitors (foliar application) on Peroxidase (PO) activity in pea under controlled condition 24

21. Fig. Effect of elicitors (foliar application) on total phenol activity in pea under controlled condition

22. Conclusions and perspective This presentation has focused on PGPR can be found in the rhizosphere . They live in association with roots and stimulate the plant growth and/or reduce the incidence of plant disease. Among the numerous PGP bacteria and fungi described up to now, the bacteria Azospirillum , Bacillus , and Pseudomonas, and the fungus Glomus are the genera more frequently mentioned on research reports. The important role that PGPR play in agriculture can be clearly deduced from the extensive research published until now.

23. Thank You