BILASTINE AND CITRIZINE IN PLASMA SAMPLES BY HPLC.docx

Reference 1

1. ESTIMATION OF BILASTINE AND CETIRIZINE IN PLASMA SAMPLES BY HPLC
Papers (130) Limitations Research Gap
Journal Article•DOI
(1) RP-HPLC Method for
Quantification of Bilastine and
Monteleukast Sodium in
Pharmaceutical Dosage form
31 Mar 2023-Research journal of
pharmacy and technology
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The limitations of the RP-HPLC method
developed in the paper were not explicitly
mentioned in the abstract.
Further details regarding any potential
limitations, challenges, or areas for
improvement in the method were not provided
in the abstract.
The paper does not mention any discussion on the
potential limitations or challenges faced during the
development and validation of the RP-HPLC method
for the simultaneous estimation of Bilastine and
Monteleukast Sodium. Providing insights into these
aspects could have added depth to the research.
There is no mention of any comparison with existing
methods or techniques for the quantification of
Bilastine and Monteleukast Sodium. Including a
discussion on the advantages or disadvantages of this
newly developed method compared to other
established methods could have highlighted the
novelty and significance of the research.

2. Papers (130) Limitations Research Gap
Open access•Journal Article•DOI
(2) Pharmacokinetics and Safety
of a Bilastine Once-Daily,
Preservative-Free, Ophthalmic
Formulation.
Dolores Ochoa  +9 more
12 Jun 2021-Advances in Therapy
1 CitationsPDF
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The study included healthy subjects with an age
range of 20-44 years, which may limit the
generalizability of the findings to a wider
population. A wider age range would have been
desirable to provide a more comprehensive
understanding of the pharmacokinetics and
safety profile of the ophthalmic formulation of
bilastine.
The study acknowledges ongoing clinical trials
are needed to further evaluate the efficacy and
safety of ophthalmic bilastine in the treatment
of allergic conjunctivitis. This suggests that
while the initial findings are promising in
healthy subjects, further research is required to
assess the effectiveness of the formulation in
patients with allergic conjunctivitis.
The study included a limited number of healthy
subjects within a specific age range (18-55 years),
which may limit the generalizability of the findings to
a broader population. A wider age range and inclusion
of patients with allergic conjunctivitis would provide
more comprehensive insights into the
pharmacokinetics and safety profile of the ophthalmic
bilastine formulation.
While the study focused on the pharmacokinetics and
safety of the ophthalmic bilastine formulation, further
research is needed to evaluate the efficacy of this
formulation in the treatment of allergic conjunctivitis.
Controlled clinical trials specifically targeting patients
with allergic conjunctivitis would be essential to
determine the clinical effectiveness of bilastine in
managing ocular symptoms associated with allergies.

3. Papers (130) Limitations Research Gap
Journal Article•DOI
(3) Accurate Estimation of Some
Pharmaceutical Compounds Using
HPLC Technology
Qasim Mezban Salih
18 Nov 2023-Asian Journal of
Applied Chemistry Research
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The paper mentions that the theoretical plates,
peak tailing, and % assay were not significantly
impacted by deliberate alterations made to the
method, indicating that the existing technique
is robust.
The low values of LOQ (Limit of Quantification)
and LOD (Limit of Detection) demonstrate the
sensitivity of the proposed approach, showing
that the method is capable of accurately
estimating pharmaceutical compounds in small
quantities.
The paper does not mention any specific research gaps
or limitations in the current study, such as areas for
further investigation or potential challenges faced
during the research process.
While the paper highlights the benefits and
advantages of the proposed HPLC method for
analyzing pharmaceutical compounds, it does not
explicitly discuss any areas where improvements or
modifications could be made to enhance the accuracy
or efficiency of the technique.

4. Papers (130) Limitations Research Gap
Journal Article•DOI
(4) Development and
Optimization of Stereoselective
Liquid Chromatographic Method
for Chiral Separation of (±)-
cetirizine and Enantiopurity
Assessment of R-levocetirizine
Valliappan Kannappan  +1 more
01 Mar 2020-Journal of Analytical
Chemistry
2 Citations
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The limitations of the study were not explicitly
mentioned in the abstract provided.
Further details regarding any limitations of the
developed method or challenges faced during
the optimization process are not specified in
the text.
The paper does not explicitly mention any discussion
on the potential limitations or challenges faced during
the development and optimization of the
stereoselective liquid chromatographic method.
Addressing these research gaps could provide insights
into the practical applicability and robustness of the
method.
While the study successfully applied the developed
method for the quantitation of cetirizine enantiomers
in pharmaceutical dosage forms, there is no mention
of any future directions or recommendations for
further research in this area. Identifying potential
areas for improvement or expansion of the study
could contribute to the advancement of chiral
separation techniques.

5. Papers (130) Limitations Research Gap
Journal Article•DOI
(5) Separation and identification
of cetirizine enantiomers in
human urine by CE and circular
dichroism independent of their
standards.
Lixia Chen  +5 more
09 Feb 2023-Journal of Separation
Science
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The study focused on cetirizine enantiomers
and their identification in human urine, limiting
the generalizability of the method to other
chiral drugs.
The method relied on the use of electronic
circular dichroism for identification, which may
require specialized equipment and expertise,
potentially limiting its widespread application
in laboratories without access to such
resources.
The paper does not mention the potential limitations
or challenges faced during the separation and
identification process of cetirizine enantiomers using
CE and circular dichroism. Addressing these issues
could provide insights into improving the
methodology.
Further exploration into the applicability of the
developed method for other chiral drugs beyond
cetirizine could be beneficial to assess its versatility
and potential for broader pharmaceutical applications.

6. Papers (130) Limitations Research Gap
Open access•Journal Article•DOI
(6) Bilastine: a lifetime
companion for the treatment of
allergies
Martin K. Church  +3 more
01 Mar 2020-Current Medical
Research and Opinion
PDF
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The paper does not specifically mention any
limitations related to the efficacy or safety of
bilastine in comparison to other antihistamines.
There is no discussion in the paper regarding
potential long-term side effects or interactions
of bilastine with other medications.
The paper does not specifically mention any research
gaps related to the use of bilastine in pediatric
populations, leaving a potential gap in knowledge
regarding its safety and efficacy in children.
Another research gap could be the long-term effects of
bilastine treatment beyond the currently available
data, as the paper focuses on its use as a lifetime
companion for allergies, suggesting the need for
further studies on its prolonged use.

7. Papers (130) Limitations Research Gap
Journal Article•DOI
(7) UV spectrophotometric
method for determination of
Bilastine in bulk and
pharmaceutical formulation
Peethala Prathyusha  +1 more
15 Feb 2020-Research Journal of
Pharmacy and Technology
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The concentration range for the UV-
spectrophotometric methods developed for the
estimation of Bilastine in bulk and
pharmaceutical formulation was limited to 10–
140 μg/ml.
The % RSD value for both intra-day and inter-
day precision was less than 2%, indicating a
limitation in the precision assessment beyond
this specified range.
The paper does not mention any discussion on the
potential limitations or challenges faced during the
development and validation of the UV-
spectrophotometric methods for Bilastine
determination.
There is no indication in the abstract of any
comparison with existing methods or studies, which
could have highlighted the novelty or advantages of
the developed UV-spectrophotometric methods.

8. Papers (130) Limitations Research Gap
Journal Article•DOI
(8) Safety of bilastine in patients
with allergic rhinitis
О.В. Себекина  +1 more
01 May 2024-Praktičeskaâ
allergologiâ
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The paper does not specifically mention any
limitations associated with the safety profile
assessment of bilastine in patients with allergic
rhinitis.
The abstract does not provide information on
any constraints or drawbacks related to the
clinical trials evaluating the safety of bilastine
compared to a placebo in patients with allergic
rhinitis.
The paper does not specifically mention any identified
research gaps within the study on the safety of
bilastine in patients with allergic rhinitis.
It does not highlight any areas where further research
is needed to enhance the understanding of the safety
or efficacy of bilastine in the treatment of allergic
rhinitis.

9. Papers (130) Limitations Research Gap
Journal Article•DOI
(9) Analytical Method
Development and Validation for
the Simultaneous Estimation of
Bilastine and Montelukast by RP-
HPLC
Boinapalli Sudhakar  +2 more
22 May 2023-Asian Journal of
Research in Pharmaceutical
Science
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The limitations of the paper are not explicitly
mentioned in the provided abstract.
As the abstract focuses on the development
and validation of the analytical method for the
simultaneous estimation of Bilastine and
Montelukast, specific limitations related to the
method or study design are not outlined.
The paper does not mention any discussion or analysis
regarding the potential limitations or challenges faced
during the method development and validation
process. Providing insights into any difficulties
encountered could have added depth to the study.
There is no mention of future research directions or
areas for further investigation related to the
simultaneous estimation of Bilastine and Montelukast.
Including suggestions for future studies could have
highlighted potential gaps in knowledge or
opportunities for advancement in this field.

10. Papers (130) Limitations Research Gap
Journal Article•DOI
(10) Pharmacokinetics of
hydroxyzine and cetirizine
following oral administration of
hydroxyzine to exercised
Thoroughbred horses.
Heather K Knych  +4 more
06 Sep 2019-Journal of Veterinary
Pharmacology and Therapeutics
2 Citations
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The study highlights the lack of published
studies suitable for establishing appropriate
regulatory recommendations regarding the use
of hydroxyzine in performance horses,
indicating a gap in knowledge in this area.
The findings suggest that a prolonged
withdrawal time should be observed if
hydroxyzine is used in performance horses, as it
is classified as a prohibited substance by the
applicable regulatory body, emphasizing the
need for careful consideration and monitoring
of its administration in such contexts.
Lack of published studies establishing appropriate
regulatory recommendations for the use of
hydroxyzine in performance horses, despite its
common usage.
The need for a prolonged withdrawal time for
hydroxyzine if administered to performance horses, as
it is classified as a prohibited substance by the
applicable regulatory body.

11. Papers (130) Limitations Research Gap
Open access•Journal Article•DOI
(11) Stability Indicating Isocratic
HPLC Method for Bilastine and
Characterization of Forced
Degradation Products by LC-
MS/MS
Dr. Shital Patel  +1 more
30 Dec 2022-International journal
of life science and pharma
research
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Bilastine was found to degrade under acid and
oxidation conditions, indicating limitations in
terms of stability under certain environmental
factors.
The study focused on the degradation products
of Bilastine under specific stress conditions,
potentially limiting the generalizability of the
findings to other degradation pathways or
environmental factors.
The paper could further explore the potential impact
of different environmental conditions on the
degradation of Bilastine, beyond just acid and
oxidation conditions.
Investigating the stability of Bilastine in the presence
of various excipients commonly found in
pharmaceutical formulations could provide valuable
insights for practical applications in drug
development.

12. Papers (130) Limitations Research Gap
Open access•Journal Article•DOI
(12) Estimation of bilastine and
montelukast by stability
indicating liquid chromatographic
method in pure binary mixture
and their marketed tablets
Anusha . Kunala  +1 more
26 Jul 2022-International journal
of health sciences
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The limitations of the paper were not explicitly
mentioned in the provided abstract.
Therefore, specific limitations regarding the
developed RP-HPLC method for the estimation
of Bilastine and Montelukast in bulk and tablets
are not outlined in the text.
The paper does not explicitly mention any specific
research gaps that were identified or addressed during
the study.
It would be beneficial for future research to explore
any limitations or areas for improvement in the
developed RP-HPLC method for the estimation of
Bilastine and Montelukast, as well as to investigate
any potential challenges or drawbacks in the stability
testing procedures employed.

13. Papers (130) Limitations Research Gap
Journal Article•DOI
(13) Solvents effect on
Spectrophotometric estimation of
Bilastine
G Vamseekrishna  +7 more
30 Aug 2023-World Journal of
Biology Pharmacy and Health
Sciences
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The study highlighted that the solvents used,
specifically Methanol and 0.1 N Acetic acid, had
a significant impact on the determination of
Bilastine, indicating that the choice of solvent
can influence the results of the
spectrophotometric estimation.
The validation parameters were subjected to
statistical analysis using the 't' test, revealing
significant differences. This suggests that the
methods developed for estimating Bilastine
may have limitations in terms of accuracy and
precision, which should be considered when
applying these methods in practice.
The research gaps in this paper include the need for
further investigation into the specific reasons behind
the significant differences observed in the validation
parameters when different solvents are used for the
spectrophotometric estimation of Bilastine.
Another research gap is the exploration of how
varying solvent conditions may impact the accuracy
and precision of the developed UV
spectrophotometric methods for the estimation of
Bilastine in both bulk and pharmaceutical dosage
forms.

14. Papers (130) Limitations Research Gap
Open access•Posted Content•DOI
(14) A green spectrofluorimetric
approach for the versatile
determination of Bilastine in real
human plasma and
pharmaceutical preparations with
content uniformity testing
22 Aug 2022
1 Citations
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The method described in the paper has a
detection limit of 2.9 ng mL − 1 and a
quantitation limit of 8.8 ng mL − 1, which may
limit its applicability for samples with
concentrations below these limits.
While the method was successful in analyzing
Bilastine in real human plasma and
pharmaceutical preparations, the paper does
not mention any specific limitations or
challenges encountered during the analysis
process.
The paper does not mention any discussion on
potential limitations or challenges faced during the
development and application of the
spectrofluorimetric method for determining Bilastine.
There is no indication in the abstract of the paper
regarding any future directions for research or areas
that could be further explored to enhance the
method's efficiency or applicability.

15. Papers (130) Limitations Research Gap
Open access•Journal Article•DOI
(15) Development and Validation
of RP-HPLC Method for the
Simultaneous Estimation of
Bilastine and Montelukast in
Tablet Dosage Form
Narmada Vallakeerthi  +5 more
16 Jun 2023-Chemical science
international journal
PDF
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The limitations of the paper include the lack of
discussion on the potential interferences from
excipients in the commercial formulations that
could affect the accuracy of the simultaneous
estimation method for Bilastine and
Montelukast.
Another limitation is the absence of detailed
information on the specific challenges faced
during the method development and validation
process, which could provide insights into the
practical difficulties encountered in
implementing the RP-HPLC technique for these
drugs.
The paper does not explicitly mention any discussion
on the potential limitations or challenges faced during
the development and validation of the RP-HPLC
method for the simultaneous estimation of Bilastine
and Montelukast. Providing insights into these aspects
could have added depth to the research.
While the paper focuses on the development and
validation of the RP-HPLC method for Bilastine and
Montelukast, it does not delve into the comparison
with other existing methods in terms of efficiency,
cost-effectiveness, or applicability. Including a
comparative analysis with other methods could have
highlighted the uniqueness and advantages of the
proposed method.

16. Papers (130) Limitations Research Gap
Open access•Journal Article•DOI
(16) Stability indicating rp-hplc
method development and
validation for simultaneous
estimation of bilastine and
montelukast in bulk and tablet
dosage form
Shabnam Khan  +3 more
30 Mar 2023-International journal
of pharmaceutical sciences and
medicine
PDF
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The limitations of the paper include the
absence of specific details regarding the
robustness study of Bilastine and Montelukast.
While the results of the robustness study are
mentioned, the specific parameters and
outcomes of this study are not provided in
detail.
Another limitation is the lack of information on
the solubility of Bilastine and Montelukast in
different solvents. Although the solubility
results are briefly mentioned, the specific
solvents used and the corresponding solubility
values are not elaborated upon in the paper.
The paper does not mention any discussion or analysis
on the potential challenges or limitations faced during
the method development and validation process for
simultaneous estimation of Bilastine and Montelukast.
There is no indication in the paper regarding any
future directions for research or areas that could be
further explored to enhance the understanding or
application of the developed HPLC method.

17. Papers (130) Limitations Research Gap
Journal Article•DOI
(17) A new simple RP-HPLC
Method development, Validation
and Forced degradation studies of
Bilastine
Khushbu K. Patel  +2 more
16 Aug 2021-Asian Journal of
Pharmaceutical Analysis
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Bilastine was found to degrade under acid and
oxidation conditions, indicating limitations in
terms of stability under certain environmental
conditions.
The method developed did not specify the
analysis of potential degradation products of
Bilastine, which could be a limitation in fully
understanding the degradation pathways and
impurities that may form.
The paper does not mention any discussion on the
potential impurities or degradation products that
could arise during the analysis of Bilastine, which
could be a research gap in terms of ensuring the
method's robustness and reliability.
There is no mention of any comparison with existing
methods for the estimation of Bilastine, which could
be a research gap in terms of demonstrating the
novelty and advantages of the developed RP-HPLC
method.
Journal Article•DOI
(18) Development and validation
of analytical method for
estimation of bilastine in bulk and
pharmaceutical (tablet) dosage
form
Chinmayee Kishor Padte  +3 more
01 Nov 2021-Journal of the Indian
The paper mentions that the developed method
for estimating bilastine in bulk and tablet
dosage form is not applicable for the estimation
of bilastine in biological samples.
Another limitation highlighted in the paper is
the lack of investigation into the degradation
products of bilastine in the tablet dosage form.
The paper does not mention any specific research gaps
that were identified during the study.
It is not clear from the information provided whether
the authors discussed any limitations or areas for
further research in the paper.

18. Papers (130) Limitations Research Gap
Chemical Society
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Open access•Journal Article•DOI
(19) Green quality by design HPLC
approach for the simultaneous
determination of Bilastine and
Montelukast
Aya Roshdy  +4 more
02 May 2023-BMC chemistry
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The limitations of the study include the focus
on only two co-formulated antihistaminic drugs
(Bilastine and Montelukast), which may not
cover the broader spectrum of antihistaminic
medications available in the market.
Another limitation is that the stability indicating
HPLC approach was primarily used to assess the
stability of Montelukast (MNT) under various
stress conditions, potentially limiting the
generalizability of the findings to other drugs or
compounds.
The paper does not explicitly mention any identified
research gaps within the study or areas for further
investigation.
It is not specified whether the authors discuss
limitations or areas where additional research is
needed in the field of simultaneous determination of
Bilastine and Montelukast using the Quality by Design
approach.

19. Papers (130) Limitations Research Gap
Journal Article•DOI
(20) Estimation and Validation of
Bilastine in Pharmaceutical
Dosage Form and in Bulk Drug by
Spectrophotometric Method
Afnaz Jahan  +2 more
06 Aug 2022-International
research journal of pharmacy
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The limitations of the paper were not explicitly
mentioned in the provided abstract.
As the abstract focuses on the development,
validation, and application of a
spectrophotometric method for the estimation
of Bilastine, specific limitations related to the
method or study design were not outlined.
The paper does not mention any specific research gaps
that were identified during the study. It focuses on the
development, validation, and application of a
spectrophotometric method for the estimation of
Bilastine in pharmaceutical dosage form and bulk
drug.
The study primarily highlights the methodology,
validation parameters, and results obtained, without
discussing any limitations or areas for further research
within the scope of the paper.

20. Papers (130) Limitations Research Gap
Open access•Journal Article•DOI
(21) Development and validation
of rp-hplc based analytical
method for simultaneous
estimation of montelukast and
bilastine in tablet dosage form
Pranav Vyas  +3 more
06 Apr 2022-Current research in
pharmaceutical sciences
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The tailing factor for bilastine was found to be
1.258, which indicates a slight tailing of the
peak, suggesting a limitation in the symmetry of
the peak for bilastine.
The LOD (Limit of Detection) for bilastine was
found to be 0.09 µg/ml, which may limit the
sensitivity of the method for detecting lower
concentrations of bilastine in the samples.
The paper does not explicitly mention any specific
research gaps that were identified during the
development and validation of the RP-HPLC method
for simultaneous estimation of montelukast and
bilastine in tablet dosage form.
It would be beneficial for future studies to potentially
explore any limitations or challenges encountered
during the method development and validation
process, as well as areas for further improvement or
optimization in the analytical method.

21. Papers (130) Limitations Research Gap
Journal Article•DOI
(22) Simultaneous Estimation of
Bilastine and Montelukast in Bulk
by Rp-hplc and Assessment of Its
Applicability in Marketed Tablet
Dosage Form
Swati M. Andhale  +1 more
24 Jan 2022-Journal of
pharmaceutical research
international
PDF
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The paper does not explicitly mention any
limitations or drawbacks associated with the
developed RP-HPLC method for the
simultaneous estimation of Bilastine and
Montelukast.
It is important to note that the absence of
explicitly stated limitations does not necessarily
mean that there are no limitations, as
limitations may exist but were not highlighted
in the abstract provided.
The paper does not explicitly mention any identified
research gaps within the study, such as areas for
further investigation or limitations that could be
addressed in future research.
It would be beneficial for future studies to explore
potential challenges or drawbacks encountered during
the development and validation of the RP-HPLC
method for Bilastine and Montelukast, as well as any
additional factors that could impact the applicability
of the method in different settings or formulations.

22. Papers (130) Limitations Research Gap
Open access•Journal Article•DOI
(23) RP-HPLC-Based Bioanalytical
Approach for Simultaneous
Quantitation of Cinnarizine and
Domperidone in Rat Plasma
Mohit Vij  +9 more
25 Feb 2023-Separations
PDF
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The limitations of the study were not explicitly
mentioned in the provided abstract.
Further details regarding any limitations of the
RP-HPLC bioanalytical approach for the
simultaneous quantitation of cinnarizine and
domperidone in rat plasma were not provided
in the abstract.
The paper does not explicitly mention any identified
research gaps within the study, potentially indicating
that the authors did not focus on highlighting specific
gaps in the existing literature related to the
simultaneous quantitation of cinnarizine and
domperidone in rat plasma using RP-HPLC.
It is important to note that the absence of explicitly
stated research gaps does not necessarily mean that
there are no areas for further investigation or
improvement in this particular research field.

23. Papers (130) Limitations Research Gap
Journal Article•DOI
(24) Development and Validation
of RP-HPLC Method for the
Estimation of Bilastine from bulk
and Formulation
Payal J. Pardeshi  +2 more
07 May 2020-Asian Journal of
Pharmaceutical Analysis
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The limitations of the paper were not explicitly
mentioned in the provided abstract.
Further details regarding any limitations of the
developed RP-HPLC method for the estimation
of Bilastine were not provided in the abstract.
The paper does not explicitly mention any identified
research gaps or areas for further study within the
scope of the developed RP-HPLC method for the
estimation of Bilastine.
It is important for future research to potentially
explore the method's applicability to other forms of
Bilastine formulations or to investigate the method's
performance under different experimental conditions
to further validate its robustness and versatility.
Journal Article•DOI
(25) A simultaneous liquid
chromatographic analysis of
niclosamide and bicalutamide in
rat plasma by protein
precipitation extraction
Manish Majumder
01 Sep 2022
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The study did not mention any specific
limitations in the abstract.
Therefore, it is not possible to provide detailed
limitations from the text provided.
The paper does not explicitly mention any specific
research gaps that were identified during the study.
It is important to note that the absence of identified
research gaps does not necessarily mean that the
study is flawless, as research gaps could exist but were
not highlighted in the abstract.

24. Papers (130) Limitations Research Gap
Journal Article•DOI
(26) A highly sensitive
spectrofluorimetric method for
the determination of bilastine in
its pharmaceutical preparations
and biological fluids.
Aya Radwan  +4 more
01 Apr 2022-Spectrochimica Acta
Part A: Molecular and
Biomolecular Spectroscopy
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The method described in the paper is limited by
the fact that it requires the use of acetonitrile
as an organic solvent, which may not be
suitable for all applications due to its toxicity
and environmental impact.
Another limitation mentioned is the need for
careful optimization of the experimental
conditions to achieve the desired sensitivity and
accuracy in the determination of bilastine.
The paper does not mention any specific research gaps
or areas for further investigation within the scope of
the study.
It is important for future research to explore potential
limitations or challenges encountered during the
development and application of the
spectrofluorimetric method for bilastine
determination in pharmaceutical preparations and
biological fluids.

25. Papers (130) Limitations Research Gap
Open access•Journal Article•DOI
(27) Stability Indicating RP-HPLC
Method Development and
Validation for Simultaneous
Estimation of Montelukast and
Bilastine in Bulk and
Pharmaceutical Dosage Form
01 Jan 2022-World journal of
pharmaceutical sciences
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The limitations of the method developed in the
paper were not explicitly mentioned. It focused
more on the accuracy, precision, and simplicity
of the RP-HPLC method for simultaneous
estimation of Bilastine and Montelukast in bulk
and tablet dosage form.
The paper highlighted the decreased retention
times and run time, indicating that the
developed method was simple and economical
for regular quality control tests in industries.
The paper does not mention any discussion or analysis
regarding the potential interferences or impurities
that could affect the accurate estimation of Bilastine
and Montelukast in the developed RP-HPLC method.
There is no information provided on the specificity of
the method towards Bilastine and Montelukast, such
as any potential cross-reactivity with other
compounds that might be present in the samples.

26. Papers (130) Limitations Research Gap
Open access•Journal Article•DOI
(28) Pharmacokinetics and Safety
of a Bilastine Once-Daily,
Preservative-Free, Ophthalmic
Formulation.
Dolores Ochoa  +9 more
12 Jun 2021-Advances in Therapy
1 CitationsPDF
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The study included healthy subjects with an age
range of 20-44 years, which may limit the
generalizability of the findings to a wider
population. A wider age range would have been
desirable to provide a more comprehensive
understanding of the pharmacokinetics and
safety profile of the ophthalmic formulation of
bilastine.
The study acknowledges ongoing clinical trials
are needed to further evaluate the efficacy and
safety of ophthalmic bilastine in the treatment
of allergic conjunctivitis. This suggests that
while the initial findings are promising in
healthy subjects, further research is required to
assess the effectiveness of the formulation in
patients with allergic conjunctivitis.
The study included a limited number of healthy
subjects within a specific age range (18-55 years),
which may limit the generalizability of the findings to
a broader population. A wider age range and inclusion
of patients with allergic conjunctivitis would provide
more comprehensive insights into the
pharmacokinetics and safety profile of the ophthalmic
bilastine formulation.
While the study focused on the pharmacokinetics and
safety of the ophthalmic bilastine formulation, further
research is needed to evaluate the efficacy of this
formulation in the treatment of allergic conjunctivitis.
Controlled clinical trials specifically targeting patients
with allergic conjunctivitis would be essential to
determine the clinical effectiveness of bilastine in
managing ocular symptoms associated with allergies.

27. Papers (130) Limitations Research Gap
Open access•Journal Article•DOI
(29) Bioequivalence studies of
cetirizine tablets using the urine
excretion data of healthy
Ghanaian male volunteers
Gideon Onuh  +5 more
01 Jan 2023-Heliyon
Chat with Paper
The study only included healthy Ghanaian male
volunteers, which may limit the generalizability
of the findings to other populations.
The bioequivalence of cetirizine tablets was
assessed using urine excretion data, which may
not fully capture the pharmacokinetic profile of
the drug.
Lack of information on the specific methodology used
for the bioequivalence studies of cetirizine tablets
with the urine excretion data of healthy Ghanaian
male volunteers
Absence of discussion on potential limitations or
challenges faced during the research process, which
could have provided insights into areas for future
research or improvements

28. Papers (130) Limitations Research Gap
Open access•Journal Article•DOI
(30) Factorial design-assisted
reverse phase HPLC–UV approach
for the concurrent estimation of
cetirizine and azelastine in
aqueous humor
Walaa Nabil Abd-AlGhafar  +3
more
01 Dec 2022-Dental science
reports
PDF
Chat with Paper
The limitations of the study were not explicitly
mentioned in the provided text.
The paper did not discuss any specific
constraints or drawbacks related to the
developed analytical method or the concurrent
estimation of cetirizine and azelastine in
aqueous humor.
The paper does not mention any discussion or
exploration of potential limitations or challenges faced
during the development and validation of the
analytical method, which could have provided insights
into areas for future research or improvement.
There is no indication in the paper of any comparison
or discussion regarding the method's applicability to
real patient samples or clinical studies, leaving a
research gap in terms of translating the developed
method to practical use in a clinical setting.

29. Papers (130) Limitations Research Gap
Open access•Journal Article•DOI
(31) Development and validation
of rp-hplc based analytical
method for simultaneous
estimation of montelukast and
bilastine in tablet dosage form
Pranav Vyas  +3 more
06 Apr 2022-Current research in
pharmaceutical sciences
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The tailing factor for bilastine was found to be
1.258, which indicates a slight tailing of the
peak, suggesting a limitation in the symmetry of
the peak for bilastine.
The LOD (Limit of Detection) for bilastine was
found to be 0.09 µg/ml, which may limit the
sensitivity of the method for detecting lower
concentrations of bilastine in the samples.
The paper does not explicitly mention any specific
research gaps that were identified during the
development and validation of the RP-HPLC method
for simultaneous estimation of montelukast and
bilastine in tablet dosage form.
It would be beneficial for future studies to potentially
explore any limitations or challenges encountered
during the method development and validation
process, as well as areas for further improvement or
optimization in the analytical method.

30. Papers (130) Limitations Research Gap
Journal Article•DOI
(32) Simultaneous Estimation of
Bilastine and Montelukast in Bulk
by Rp-hplc and Assessment of Its
Applicability in Marketed Tablet
Dosage Form
Swati M. Andhale  +1 more
24 Jan 2022-Journal of
pharmaceutical research
international
PDF
Chat with Paper
The paper does not explicitly mention any
limitations or drawbacks associated with the
developed RP-HPLC method for the
simultaneous estimation of Bilastine and
Montelukast.
It is important to note that the absence of
explicitly stated limitations does not necessarily
mean that there are no limitations, as
limitations may exist but were not highlighted
in the abstract provided.
The paper does not explicitly mention any identified
research gaps within the study, such as areas for
further investigation or limitations that could be
addressed in future research.
It would be beneficial for future studies to explore
potential challenges or drawbacks encountered during
the development and validation of the RP-HPLC
method for Bilastine and Montelukast, as well as any
additional factors that could impact the applicability
of the method in different settings or formulations.

31. Papers (130) Limitations Research Gap
Open access•Journal Article•DOI
(33) RP-HPLC-Based Bioanalytical
Approach for Simultaneous
Quantitation of Cinnarizine and
Domperidone in Rat Plasma
Mohit Vij  +9 more
25 Feb 2023-Separations
PDF
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The limitations of the study were not explicitly
mentioned in the provided abstract.
Further details regarding any limitations of the
RP-HPLC bioanalytical approach for the
simultaneous quantitation of cinnarizine and
domperidone in rat plasma were not provided
in the abstract.
The paper does not explicitly mention any identified
research gaps within the study, potentially indicating
that the authors did not focus on highlighting specific
gaps in the existing literature related to the
simultaneous quantitation of cinnarizine and
domperidone in rat plasma using RP-HPLC.
It is important to note that the absence of explicitly
stated research gaps does not necessarily mean that
there are no areas for further investigation or
improvement in this particular research field.

32. Papers (130) Limitations Research Gap
Journal Article•DOI
(34) Development and Validation
of RP-HPLC Method for the
Estimation of Bilastine from bulk
and Formulation
Payal J. Pardeshi  +2 more
07 May 2020-Asian Journal of
Pharmaceutical Analysis
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The limitations of the paper were not explicitly
mentioned in the provided abstract.
Further details regarding any limitations of the
developed RP-HPLC method for the estimation
of Bilastine were not provided in the abstract.
The paper does not explicitly mention any identified
research gaps or areas for further study within the
scope of the developed RP-HPLC method for the
estimation of Bilastine.
It is important for future research to potentially
explore the method's applicability to other forms of
Bilastine formulations or to investigate the method's
performance under different experimental conditions
to further validate its robustness and versatility.
Journal Article•DOI
(35) A simultaneous liquid
chromatographic analysis of
niclosamide and bicalutamide in
rat plasma by protein
precipitation extraction
Manish Majumder
01 Sep 2022
Chat with Paper
The study did not mention any specific
limitations in the abstract.
Therefore, it is not possible to provide detailed
limitations from the text provided.
The paper does not explicitly mention any specific
research gaps that were identified during the study.
It is important to note that the absence of identified
research gaps does not necessarily mean that the
study is flawless, as research gaps could exist but were
not highlighted in the abstract.

33. Papers (130) Limitations Research Gap
Journal Article•DOI
(36) A highly sensitive
spectrofluorimetric method for
the determination of bilastine in
its pharmaceutical preparations
and biological fluids.
Aya Radwan  +4 more
01 Apr 2022-Spectrochimica Acta
Part A: Molecular and
Biomolecular Spectroscopy
Chat with Paper
The method described in the paper is limited by
the fact that it requires the use of acetonitrile
as an organic solvent, which may not be
suitable for all applications due to its toxicity
and environmental impact.
Another limitation mentioned is the need for
careful optimization of the experimental
conditions to achieve the desired sensitivity and
accuracy in the determination of bilastine.
The paper does not mention any specific research gaps
or areas for further investigation within the scope of
the study.
It is important for future research to explore potential
limitations or challenges encountered during the
development and application of the
spectrofluorimetric method for bilastine
determination in pharmaceutical preparations and
biological fluids.

34. Papers (130) Limitations Research Gap
Open access•Journal Article•DOI
(37) Stability Indicating RP-HPLC
Method Development and
Validation for Simultaneous
Estimation of Montelukast and
Bilastine in Bulk and
Pharmaceutical Dosage Form
01 Jan 2022-World journal of
pharmaceutical sciences
Chat with Paper
The limitations of the method developed in the
paper were not explicitly mentioned. It focused
more on the accuracy, precision, and simplicity
of the RP-HPLC method for simultaneous
estimation of Bilastine and Montelukast in bulk
and tablet dosage form.
The paper highlighted the decreased retention
times and run time, indicating that the
developed method was simple and economical
for regular quality control tests in industries.
The paper does not mention any discussion or analysis
regarding the potential interferences or impurities
that could affect the accurate estimation of Bilastine
and Montelukast in the developed RP-HPLC method.
There is no information provided on the specificity of
the method towards Bilastine and Montelukast, such
as any potential cross-reactivity with other
compounds that might be present in the samples.

35. Papers (130) Limitations Research Gap
Open access•Journal Article•DOI
(38) Pharmacokinetics and Safety
of a Bilastine Once-Daily,
Preservative-Free, Ophthalmic
Formulation.
Dolores Ochoa  +9 more
12 Jun 2021-Advances in Therapy
1 CitationsPDF
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The study included healthy subjects with an age
range of 20-44 years, which may limit the
generalizability of the findings to a wider
population. A wider age range would have been
desirable to provide a more comprehensive
understanding of the pharmacokinetics and
safety profile of the ophthalmic formulation of
bilastine.
The study acknowledges ongoing clinical trials
are needed to further evaluate the efficacy and
safety of ophthalmic bilastine in the treatment
of allergic conjunctivitis. This suggests that
while the initial findings are promising in
healthy subjects, further research is required to
assess the effectiveness of the formulation in
patients with allergic conjunctivitis.
The study included a limited number of healthy
subjects within a specific age range (18-55 years),
which may limit the generalizability of the findings to
a broader population. A wider age range and inclusion
of patients with allergic conjunctivitis would provide
more comprehensive insights into the
pharmacokinetics and safety profile of the ophthalmic
bilastine formulation.
While the study focused on the pharmacokinetics and
safety of the ophthalmic bilastine formulation, further
research is needed to evaluate the efficacy of this
formulation in the treatment of allergic conjunctivitis.
Controlled clinical trials specifically targeting patients
with allergic conjunctivitis would be essential to
determine the clinical effectiveness of bilastine in
managing ocular symptoms associated with allergies.

36. Papers (130) Limitations Research Gap
Open access•Journal Article•DOI
(39) Bioequivalence studies of
cetirizine tablets using the urine
excretion data of healthy
Ghanaian male volunteers
Gideon Onuh  +5 more
01 Jan 2023-Heliyon
Chat with Paper
The study only included healthy Ghanaian male
volunteers, which may limit the generalizability
of the findings to other populations.
The bioequivalence of cetirizine tablets was
assessed using urine excretion data, which may
not fully capture the pharmacokinetic profile of
the drug.
Lack of information on the specific methodology used
for the bioequivalence studies of cetirizine tablets
with the urine excretion data of healthy Ghanaian
male volunteers
Absence of discussion on potential limitations or
challenges faced during the research process, which
could have provided insights into areas for future
research or improvements

37. Papers (130) Limitations Research Gap
Open access•Journal Article•DOI
(40) Factorial design-assisted
reverse phase HPLC–UV approach
for the concurrent estimation of
cetirizine and azelastine in
aqueous humor
Walaa Nabil Abd-AlGhafar  +3
more
01 Dec 2022-Dental science
reports
PDF
Chat with Paper
The limitations of the study were not explicitly
mentioned in the provided text.
The paper did not discuss any specific
constraints or drawbacks related to the
developed analytical method or the concurrent
estimation of cetirizine and azelastine in
aqueous humor.
The paper does not mention any discussion or
exploration of potential limitations or challenges faced
during the development and validation of the
analytical method, which could have provided insights
into areas for future research or improvement.
There is no indication in the paper of any comparison
or discussion regarding the method's applicability to
real patient samples or clinical studies, leaving a
research gap in terms of translating the developed
method to practical use in a clinical setting.

38. Papers (130) Limitations Research Gap
Journal Article•DOI
(41) Bilastine: Quantitative
Determination by LC with
Fluorescence Detection and
Structural Elucidation of the
Degradation Products Using
HRMS.
Paola Ribeiro Motta  +6 more
01 Nov 2020-Journal of AOAC
International
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The paper did not mention any specific
limitations of the developed LC stability-
indicating method for the quantitative
determination of bilastine in coated tablets.
However, the toxicity studies revealed the high
mutagenic potential of the degradation product
benzimidazole (DP1) and the hepatotoxicity and
hERG I inhibitor effects of the degradation
product amine N-oxide of bilastine (DP2),
indicating potential safety concerns associated
with these degradation products.
The paper does not mention any specific discussion or
analysis regarding the potential factors contributing to
the formation of the identified degradation products,
benzimidazole and amine N-oxide of bilastine. Further
research could explore the mechanisms or conditions
leading to the generation of these degradation
products.
While the paper briefly mentions in silico toxicity
studies on the degradation products, it does not delve
into the detailed methodology or results of these
studies. Future research could focus on conducting
comprehensive in silico toxicity assessments to better
understand the potential health risks associated with
the identified degradation products.

39. Papers (130) Limitations Research Gap
Journal Article•DOI
(42) A new stability indicating RP-
HPLC method for determination
of Bilastine in bulk and
pharmaceutical formulation
Peethala Prathyusha  +3 more
23 Jun 2020-Research Journal of
Pharmacy and Technology
Chat with Paper
The limitations of the study were not explicitly
mentioned in the abstract provided.
Further details regarding any limitations of the
developed RP-HPLC method for the
determination of Bilastine in bulk and
pharmaceutical dosage form were not specified
in the abstract.
The paper does not mention any specific discussion on
the potential impurities or degradation products of
Bilastine that could affect its stability, which could be
a research gap in terms of understanding the
compound's degradation pathways.
There is no mention of the method's applicability to
real-time monitoring of Bilastine in complex biological
matrices, which could be an area for further research
to assess the method's practical utility in clinical or
pharmacokinetic studies.

40. Papers (130) Limitations Research Gap
Journal Article•DOI
(43) Bioanalytical Method
Development and Validation of
Eprosartan Mesylate and
Hydrochlorthiazide using RP-HPLC
in Human plasma
Gurumurthy. Telugu  +1 more
31 Mar 2023-Research journal of
pharmacy and technology
Chat with Paper
The limitations of the study were not explicitly
mentioned in the abstract provided.
Therefore, specific limitations such as
challenges faced during method development,
validation, or analysis were not outlined in the
text.
The paper does not mention any discussion or
exploration of potential limitations or challenges faced
during the method development and validation
process, which could be considered a research gap.
There is no indication in the abstract of any
comparison with existing methods or techniques for
analyzing Eprosartan mesylate and
Hydrochlorothiazide in human plasma, which could be
an area for further research to validate the
effectiveness and efficiency of the newly developed
RP-HPLC method.

41. Papers (130) Limitations Research Gap
Open access•Journal Article•DOI
(44) Bioanalytical method process
of chromatographic analysis of
tizanidine in the formulation and
human plasma
01 Jan 2023-Rasayan journal of
Chemistry
PDF
Chat with Paper
The limitations of the paper include a lower
linearity observed in the spiking studies of
Tizanidine in human plasma, with R2 = 0.8155,
compared to the R2 value of 0.99979 obtained
in the linearity studies of Tizanidine in the API
(10-50 mcg/mL).
Another limitation highlighted is the need for
further optimization or exploration of
conditions to improve the linearity and
robustness of the method, especially in the
context of human plasma analysis, to enhance
the accuracy and reliability of the quantitative
analyses performed.
The paper lacks discussion on the potential challenges
or limitations faced during the development and
validation of the bioanalytical method for
chromatographic analysis of tizanidine. Addressing
these challenges could provide insights into the
practical applicability and robustness of the method.
There is no mention of future directions or
recommendations for further research in the field of
bioanalytical methods for tizanidine analysis.
Providing suggestions for future studies could help in
advancing the understanding and optimization of
analytical techniques for tizanidine quantification.

42. Papers (130) Limitations Research Gap
Open access•Journal Article•DOI
(45) Development and Validation
RP-HPLC Method for
Simultaneous Estimation of
Bilastine and Montelukast in Bulk
and Pharmaceutical Dosage Form
S. Nizamuddin  +1 more
16 Apr 2022-Asian Pacific journal
of health sciences
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- The paper does not mention any specific research gaps
or limitations in the study, which could have provided
insights into areas for future research or
improvements in the method.
Further exploration into the robustness of the method
under different conditions or with different sample
matrices could have been beneficial to enhance the
applicability and reliability of the developed RP-HPLC
method.

43. Papers (130) Limitations Research Gap
Open access•Journal Article•DOI
(46) Bilastine: stability-indicating
a method using environmentally
friendly by reversed-phase high-
performance liquid
chromatography (RP-HPLC)
Paulo Roberto Rodrigues
Martini  +5 more
28 Jul 2022-Drug Analytical
Research
PDF
Chat with Paper
The limitations of the paper were not explicitly
mentioned in the provided text.
The paper focused on the development and
validation of an environmentally friendly
analytical method for the determination of
bilastine in coated tablets, highlighting its
stability-indicating properties and eco-friendly
aspects without discussing any specific
limitations.
The paper mentions that this study is the first report
of bilastine determination using green approach
principles, indicating a research gap in the field of
environmentally friendly methods for quantification of
bilastine content.
While the method developed in the study is validated
and shows stability-indicating properties for bilastine
in coated tablets, there is a potential research gap in
exploring the application of this method to routine
analysis in pharmaceutical settings.

44. Papers (130) Limitations Research Gap
Journal Article•DOI
(47) Ocular biodistribution of
once daily bilastine eye drops
‐
reveals highest levels in
conjunctiva up to 24   hours post
‐

administration
Anna Gonzalo  +3 more
01 Jan 2022-Acta
Ophthalmologica
2 Citations
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Absorption of bilastine from the eye drops was
limited, indicating potential challenges in
achieving systemic effects through this route of
administration.
The study focused on the ocular biodistribution
of bilastine in rabbits, which may not directly
translate to human responses, highlighting the
need for further studies in human subjects to
confirm the findings.
The paper does not discuss the potential long-term
effects or repeated use of the bilastine eye drops on
ocular tissues beyond the 24-hour post-administration
period.
The study does not address the comparison of the
biodistribution and pharmacokinetics of bilastine eye
drops with other commonly used antihistamine eye
drop formulations, limiting the ability to assess the
uniqueness or advantages of the developed bilastine
formulation.

45. Papers (130) Limitations Research Gap
Journal Article•DOI
(48) UV spectrophotometric
method for determination of
Bilastine in bulk and
pharmaceutical formulation
Peethala Prathyusha  +1 more
15 Feb 2020-Research Journal of
Pharmacy and Technology
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The concentration range for the UV-
spectrophotometric methods developed for the
estimation of Bilastine in bulk and
pharmaceutical formulation was limited to 10–
140 μg/ml.
The % RSD value for both intra-day and inter-
day precision was less than 2%, indicating a
limitation in the precision assessment beyond
this specified range.
The paper does not mention any discussion on the
potential limitations or challenges faced during the
development and validation of the UV-
spectrophotometric methods for Bilastine
determination.
There is no indication in the abstract of any
comparison with existing methods or studies, which
could have highlighted the novelty or advantages of
the developed UV-spectrophotometric methods.

46. Papers (130) Limitations Research Gap
Open access•Posted Content•DOI
(49) Spectroscopy- based
development and validation of
analytical technique for
simultaneous estimation of
bilastine montelukast
Craig Stephen
10 Aug 2022
Chat with Paper
The paper discusses the analytical methods for
Bilastine and Montelukast Sodium individually
but does not provide any methods for
combining the two substances.
The research highlights the lack of available
ways to combine Bilastine with Montelukast
Sodium, indicating a limitation in the current
analytical techniques for simultaneous
estimation of both substances.
The research paper highlights the discussion on
analytical methods for Bilastine and Montelukast
Sodium individually, but there is a gap in the
availability of methods for combining these two
substances.
The paper mentions the use of UV spectroscopy for
testing Bilastine and Montelukast Sodium, indicating a
potential research gap in the development of new
analytical techniques combining these two
compounds.

47. Papers (130) Limitations Research Gap
Journal Article•DOI
(50) Plasma and urine
pharmacokinetics of hydroxyzine
and cetirizine following repeated
oral administrations to exercised
horses.
Stuart W. Paine  +4 more
01 Sep 2021-Journal of Veterinary
Pharmacology and Therapeutics
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The study acknowledges that while the
developed pharmacokinetic model and
screening limits can provide a detection time of
4 days for hydroxyzine and cetirizine in plasma
or urine, the actual implementation and
regulation of these limits ultimately depend on
individual racing authorities and their risk
management strategies.
The research highlights the importance of
considering the statistical protection of the
detection time by providing concentration
prediction intervals for the 80th, 95th, and 99th
percentiles of a virtual horse population.
However, the decision on whether these
intervals offer sufficient protection against
potential doping violations is left to the
discretion of the regulatory bodies.
The paper highlights the need for further research on
the detection times (DT) of hydroxyzine and cetirizine
in plasma and urine in order to better inform trainers
and veterinary surgeons prescribing these substances
for Thoroughbred racehorses.
The study emphasizes the importance of individual
racing authorities applying their own risk management
strategies, indicating a potential research gap in the
standardization of regulations regarding the use of
antihistamine drugs in equine sporting events.

48. Papers (130) Limitations Research Gap
Journal Article•DOI
(51) Degradation kinetics of
Bilastine determined by RP-HPLC
method and identification of its
degradation product in oxidative
condition
Radia Ouarezki  +2 more
01 Apr 2020-Chemical Papers
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The limitations of the study were not explicitly
mentioned in the abstract provided.
Further details regarding any limitations of the
research were not outlined in the text.
The paper does not mention any investigation into the
potential impact of different storage conditions on the
degradation kinetics of Bilastine, which could be a
valuable area for future research to explore the
stability of the drug under various environmental
conditions.
There is no discussion on the potential influence of
different excipients commonly used in tablet
formulations on the oxidative degradation of Bilastine.
Further studies could focus on understanding how
different excipients may affect the stability of the drug
in tablet form.

49. Papers (130) Limitations Research Gap
Open access•Journal Article•DOI
(52) Development and validation
of the rp-hplc method for
estimation of cilnidipine in rat
plasma
Ram Mohan Jaiswal  +1 more
01 Oct 2022-International Journal
of Pharmacy and Pharmaceutical
Sciences
PDF
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The limitations of the paper were not explicitly
mentioned in the provided abstract.
As the abstract does not specify any limitations,
it is unclear what specific constraints or
challenges were encountered during the
development and validation of the RP-HPLC
method for estimating cilnidipine in rat plasma.
The paper does not explicitly mention any specific
research gaps identified within the study, which could
include areas where further investigation or
improvement is needed in the methodology, results,
or implications of the research.
Future studies could focus on exploring the application
of the developed RP-HPLC method for detecting
cilnidipine in other biological matrices or in different
animal models to assess its broader applicability and
potential limitations.

50. Papers (130) Limitations Research Gap
Open access•Journal Article•DOI
(53) QbD-driven HPLC method for
the quantification of rivastigmine
in rat plasma and brain for
pharmacokinetics study
Divya Gopalan  +6 more
05 Jun 2022-journal of applied
pharmaceutical science
PDF
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The paper does not mention any specific
limitations or drawbacks of the developed HPLC
method for the quantification of rivastigmine in
rat plasma and brain matrices.
No limitations related to the applicability or
performance of the method in the
pharmacokinetics study of rivastigmine in
Sprague Dawley rats are discussed in the paper.
The paper highlights the lack of an available HPLC-UV
method utilizing protein precipitation for drug
extraction in the bioanalysis of Rivastigmine (RST) in
both plasma and brain matrices, indicating a research
gap in the field of pharmacokinetics studies.
The study also emphasizes the need for further
research on the nasal pharmacokinetics of RST in
Sprague Dawley rats, suggesting a gap in
understanding the applicability of the developed
quantification method in biological matrices for this
specific route of administration.

51. Papers (130) Limitations Research Gap
Open access•Journal Article•DOI
(54) A Novel and Rapid RP-HPLC
Quantitative Method for the
estimation of Canagliflozin in
Human Plasma
Ajitha A  +2 more
06 Jan 2021-International Journal
of Research in Pharmaceutical
Sciences
2 Citations
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The limitations of the paper were not explicitly
mentioned in the provided abstract.
As the abstract does not specify any limitations,
it is recommended to refer to the full text of the
paper for a comprehensive understanding of
any potential limitations discussed by the
authors.
The paper does not mention any discussion or
identification of potential limitations or challenges
faced during the development and validation of the
RP-HPLC method for the estimation of Canagliflozin in
human plasma. Addressing these research gaps could
provide valuable insights into the practical
applicability and robustness of the proposed method.
There is no explicit mention of comparisons with
existing methods or techniques for the quantification
of Canagliflozin in human plasma. Including a
discussion on the advantages or disadvantages of the
developed RP-HPLC method compared to other
established methods could help in understanding the
novelty and significance of the proposed approach.

52. Papers (130) Limitations Research Gap
Journal Article•DOI
(55) New HPLC–MS method for
rapid and simultaneous
quantification of doxycycline,
diethylcarbamazine and
albendazole metabolites in rat
plasma and organs after
concomitant oral administration
Andi Dian Permana  +3 more
05 Jun 2019-Journal of
Pharmaceutical and Biomedical
Analysis
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The study mentions that the method developed
for simultaneous quantification of doxycycline,
diethylcarbamazine, and albendazole
metabolites was validated for plasma and
organ samples, but there is no mention of
validation for other biological matrices such as
urine or feces.
While the stability tests showed excellent
results for autosampler bench-top, long-term
storage, and freeze-thaw stability, there is no
specific mention of stability testing under
conditions like light exposure or different
temperature variations, which could be
potential limitations in real-world scenarios.
The paper highlights the development of a new HPLC-
MS method for the simultaneous quantification of
doxycycline, diethylcarbamazine, and albendazole
metabolites in rat plasma and organs after
concomitant oral administration. However, the
authors do not specifically mention any identified
research gaps within the scope of their study.
While the paper successfully validates the method
according to ICH and FDA guidelines and applies it to
pharmacokinetic and biodistribution studies, it does
not explicitly discuss any limitations or areas for
further research within the context of their findings.
Open access•Journal Article•DOI
(56) Application of RP-HPLC
method for the simultaneous
determination of cetirizine in the
presence of quinolones
The paper mentions that the method developed
is not applicable for the determination of
ciprofloxacin and ofloxacin in the presence of
cetirizine due to interference.
Another limitation highlighted is the lack of
investigation into the degradation kinetics of
Lack of investigation into the potential interference of
other commonly co-administered drugs on the
simultaneous determination of cetirizine and
quinolones using RP-HPLC method.
Absence of discussion on the practical implications
and limitations of the proposed RP-HPLC method in

53. Papers (130) Limitations Research Gap
Hina Shamshad  +1 more
01 Dec 2021-Future Journal of
Pharmaceutical Sciences
3 CitationsPDF
Chat with Paper
cetirizine in the presence of quinolones. real-world pharmaceutical analysis scenarios.
Open access•Journal Article•DOI
(57) A New HPLC Stability
Indicating Method and Validation
for Simultaneous Quantitation of
Bilastine and Montelukast
Sodium in API and Marketed
Formulation by QbD Approach
Kamini Sethy  +2 more
01 Jan 2022-Asian Journal of
Chemistry
1 Citations
Chat with Paper
The limitations of the paper were not explicitly
mentioned in the provided abstract.
Further details regarding any limitations of the
developed HPLC stability indicating method
were not specified in the abstract.
The paper does not explicitly mention any identified
research gaps within the study, focusing instead on
the development and validation of the new HPLC
stability indicating method for simultaneous
quantitation of bilastine and montelukast sodium in a
marketed tablet dosage form.
It is important to note that the absence of discussion
on research gaps does not necessarily indicate that
there are none, as research gaps may exist but were
not highlighted in the abstract of the paper.

54. Papers (130) Limitations Research Gap
Journal Article•DOI
(58) Accurate Estimation of Some
Pharmaceutical Compounds Using
HPLC Technology
Qasim Mezban Salih
18 Nov 2023-Asian Journal of
Applied Chemistry Research
Chat with Paper
The paper mentions that the theoretical plates,
peak tailing, and % assay were not significantly
impacted by deliberate alterations made to the
method, indicating that the existing technique
is robust.
The low values of LOQ (Limit of Quantification)
and LOD (Limit of Detection) demonstrate the
sensitivity of the proposed approach, showing
that the method is capable of accurately
estimating pharmaceutical compounds in small
quantities.
The paper does not mention any specific research gaps
or limitations in the current study, such as areas for
further investigation or potential challenges faced
during the research process.
While the paper highlights the benefits and
advantages of the proposed HPLC method for
analyzing pharmaceutical compounds, it does not
explicitly discuss any areas where improvements or
modifications could be made to enhance the accuracy
or efficiency of the technique.

55. Papers (130) Limitations Research Gap
Journal Article•DOI
(59) Chiral separation of five
antihistamine drug enantiomers
and enantioselective
pharmacokinetic study of
carbinoxamine in rat plasma by
HPLC-MS/MS
Meng Li  +5 more
15 Apr 2020-New Journal of
Chemistry
15 Citations
Chat with Paper
The limitations of the study were not explicitly
mentioned in the abstract provided.
Further details on any limitations encountered
during the chiral separation process, method
development, validation, or pharmacokinetic
study were not specified in the abstract.
The paper could further investigate the potential
reasons behind the lack of stereoselectivity in the
absorption and elimination processes of
carbinoxamine enantiomers in rats, as well as explore
the factors that may contribute to the absence of
chiral inversion.
Future research could focus on expanding the
pharmacokinetic study to other animal models or even
human subjects to determine if the findings regarding
carbinoxamine enantiomers hold true across different
species.

56. Papers (130) Limitations Research Gap
Journal Article•DOI
(60) Bio-analytical Method for the
Pre-clinical Estimation of
Donepezil Hydrochloride in
Rabbit Plasma by RP-HPLC
Venna R Surya Anusha  +3 more
30 Nov 2023-Research Journal of
Pharmacy and Technology
Chat with Paper
The limitations of the study were not explicitly
mentioned in the abstract provided.
Further details regarding any limitations of the
developed RP-HPLC bioanalytical method for
the estimation of donepezil HCl in rabbit
plasma would require a more in-depth review
of the full paper.
The paper does not mention any specific discussion on
potential limitations or challenges faced during the
development and validation of the RP-HPLC
bioanalytical method for the estimation of donepezil
HCl in rabbit plasma. Addressing these limitations
could provide insights into areas for further
improvement or refinement of the method.
There is no mention of comparisons with other
existing methods for the estimation of donepezil HCl
in rabbit plasma. Including a discussion on the
advantages or disadvantages of this newly developed
method compared to other established methods could
help in identifying areas where this method excels or
where further enhancements are needed.

57. Papers (130) Limitations Research Gap
Open access•Journal Article•DOI
(61) Bilastine - Novel anti
histamine drug for allergic rhinitis
Abhijit Trailokya  +1 more
15 Apr 2022-Indian journal of
immunology and respiratory
medicine
Chat with Paper
The paper does not specifically mention any
limitations regarding the use of Bilastine in
pediatric populations or in pregnant or
breastfeeding women. Further studies may be
needed to determine the safety and efficacy of
Bilastine in these specific patient groups.
The paper does not discuss potential drug
interactions or contraindications with other
medications that patients may be taking
concurrently with Bilastine for allergic rhinitis
or other allergic conditions. Understanding
these interactions is important for ensuring the
safe use of Bilastine in clinical practice.
The paper does not specifically address any research
gaps in the field of allergic rhinitis or the treatment of
allergic diseases with Bilastine.
There is no mention of future research directions or
areas that need further investigation in relation to the
use of Bilastine as a treatment for allergic rhinitis.

58. Papers (130) Limitations Research Gap
Journal Article•DOI
(62) Development and
Optimization of Stereoselective
Liquid Chromatographic Method
for Chiral Separation of (±)-
cetirizine and Enantiopurity
Assessment of R-levocetirizine
Valliappan Kannappan  +1 more
01 Mar 2020-Journal of Analytical
Chemistry
2 Citations
Chat with Paper
The limitations of the study were not explicitly
mentioned in the abstract provided.
Further details regarding any limitations of the
developed method or challenges faced during
the optimization process are not specified in
the text.
The paper does not explicitly mention any discussion
on the potential limitations or challenges faced during
the development and optimization of the
stereoselective liquid chromatographic method.
Addressing these research gaps could provide insights
into the practical applicability and robustness of the
method.
While the study successfully applied the developed
method for the quantitation of cetirizine enantiomers
in pharmaceutical dosage forms, there is no mention
of any future directions or recommendations for
further research in this area. Identifying potential
areas for improvement or expansion of the study
could contribute to the advancement of chiral
separation techniques.

59. Papers (130) Limitations Research Gap
Open access•Journal Article•DOI
(63) Simultaneous determination
of levocetirizine dihydrochloride
and montelukast sodium in
human plasma by LC–MS/MS:
Development, validation, and
application to a human
pharmacokinetic study
Noha S. Rashed  +1 more
01 Sep 2019-Acta
Chromatographica
2 CitationsPDF
Chat with Paper
The paper mentions that interference arising
from endogenous substances can exist in much
higher concentration than the drugs of interest,
potentially leading to high imprecision and loss
of sensitivity in the ionization of the drugs.
The study highlights the importance of
specificity in the LC-MS/MS method due to the
possibility of co-elution of endogenous
substances with the drugs of interest, which
could impact the accuracy and reliability of the
results.
The paper does not explicitly mention any specific
research gaps or limitations in the study, such as
potential challenges faced during method
development or areas for further improvement in the
analytical technique.
While the study successfully developed a sensitive and
reliable LC-MS/MS method for simultaneous
determination of levocetirizine dihydrochloride and
montelukast sodium in human plasma, it does not
discuss any future directions for expanding the
application of the method to other matrices or
potential modifications to enhance its efficiency or
sensitivity.

60. Papers (130) Limitations Research Gap
Journal Article
(64) Bioequivalence of
Levocetirizine Hydrochloride
Granules (Kangzhitai) in Healthy
Subjects.
01 May 2023
Chat with Paper
The study had a limited sample size of only
twenty healthy male subjects, which may not
fully represent the general population in terms
of demographics, health conditions, or
potential variations in drug response.
The study focused on healthy individuals, so the
findings may not be directly applicable to
individuals with underlying health conditions or
specific patient populations that may react
differently to the drugs.
The bioavailability of levocetirizine hydrochloride
granules, specifically Kangzhitai®, had not been
previously determined, indicating a research gap in
understanding the pharmacokinetics of this
formulation.
While the study confirmed the bioequivalence of
Kangzhitai® and Xyzal®, further research could explore
potential differences in efficacy or safety profiles
between the two drugs, providing valuable insights for
clinical practice.

61. Papers (130) Limitations Research Gap
Open access•Journal Article•DOI
(65) Stability indicating rp-hplc
method for estimation of
repaglinide in rabbit plasma
Raja Navamanisubramanian  +4
more
20 Apr 2019-International Journal
of Applied Pharmaceutics
PDF
Chat with Paper
The limitations mentioned in the paper include
the need for further investigation into the
potential interfering peaks in the blank
chromatograms of plasma samples and the
chromatograms of plasma spiked with both REP
and IS to ensure accurate analysis and
interpretation of results.
Another limitation highlighted is the
importance of maintaining consistency in the
percentage recovery during the liquid
extraction process, as variations in recovery
could impact the accuracy and reliability of the
method for estimating REP in rabbit plasma.
The paper does not explicitly mention any specific
research gaps or areas for further investigation, which
could limit the identification of potential gaps in the
study.
It would be beneficial for future research to address
any limitations or challenges encountered during the
development and validation of the RP-HPLC method
for estimating repaglinide in rabbit plasma, as this
could help in improving the method or expanding its
applicability.

62. Papers (130) Limitations Research Gap
Open access•Journal Article•DOI
(66) Elucidation of Inverse Agonist
Activity of Bilastine.
Hiroyuki Mizuguchi  +9 more
08 Jun 2020-Pharmaceutics
8 Citations
Chat with Paper
The paper mentions that there is no
information available to demonstrate the
inverse agonist activity of bilastine, including
inositol phosphates accumulation.
It is stated that the inverse agonist activity of
bilastine is yet to be elucidated, indicating a gap
in the current understanding of this aspect of
the drug.
The research paper highlights a gap in knowledge
regarding the inverse agonist activity of bilastine,
specifically in terms of inositol phosphates
accumulation. The paper mentions that while bilastine
has been identified as a non-sedative antihistamine
satisfying requirements for oral antihistamines, its
inverse agonist activity, including inositol phosphates
accumulation, has not been elucidated.
Another research gap identified in the paper is the
need to further investigate the potential of bilastine as
an inverse agonist. The study indicates that bilastine
demonstrated characteristics of an inverse agonist by
suppressing basal H1R gene expression in a dose-
dependent manner, suggesting a potential for
preseasonal prophylactic administration to down-
regulate basal H1R gene expression in the nasal
mucosa and alleviate nasal symptoms during peak
pollen periods.

63. Papers (130) Limitations Research Gap
Journal Article•DOI
(67) Simultaneous Determination
of Chloroquine and
Pyrimethamine with Cetirizine in
an Active Form and Human Serum
by RP-HPLC.
Hina Shamshad  +3 more
29 Oct 2021-Journal of
Chromatographic Science
9 Citations
Chat with Paper
The limitations of the study were not explicitly
mentioned in the abstract provided.
Further details regarding any limitations of the
developed RP-HPLC method for the
simultaneous determination of chloroquine,
pyrimethamine, and cetirizine in bulk drug and
human serum were not specified in the
abstract.
The paper does not mention any specific discussion on
potential limitations or challenges encountered during
the development and validation of the RP-HPLC
method for simultaneous determination of
chloroquine, pyrimethamine, and cetirizine
hydrochloride. Addressing these research gaps could
provide insights into the practical applicability and
robustness of the method.
There is no explicit mention of future directions or
areas for further research in the abstract. Including
suggestions for future studies could help in expanding
the scope of the research and exploring additional
applications or enhancements of the developed
method.

64. Papers (130) Limitations Research Gap
Open access•Journal Article•DOI
(68) Bilastine: a lifetime
companion for the treatment of
allergies
Martin K. Church  +3 more
01 Mar 2020-Current Medical
Research and Opinion
PDF
Chat with Paper
The paper does not specifically mention any
limitations related to the efficacy or safety of
bilastine in comparison to other antihistamines.
There is no discussion in the paper regarding
potential long-term side effects or interactions
of bilastine with other medications.
The paper does not specifically mention any research
gaps related to the use of bilastine in pediatric
populations, leaving a potential gap in knowledge
regarding its safety and efficacy in children.
Another research gap could be the long-term effects of
bilastine treatment beyond the currently available
data, as the paper focuses on its use as a lifetime
companion for allergies, suggesting the need for
further studies on its prolonged use.
Journal Article•DOI
(69) Development and validation
of RP-HPLC based bioanalytical
method for simultaneous
estimation of curcumin and
quercetin in rat's plasma
01 Sep 2022-South African Journal
of Botany
7 Citations
The paper mentions that the method developed
is specific for curcumin and quercetin and may
not be applicable for other compounds.
Another limitation highlighted is the need for
further studies to explore the method's
applicability in different biological matrices and
under different experimental conditions.
The paper does not mention any specific research gaps
that were identified during the study.
It is not clear from the information provided whether
the authors discussed any limitations or areas for
further research in the paper.

65. Papers (130) Limitations Research Gap
Chat with Paper
Journal Article•DOI
(70) Stability indicating RP-HPLC
method for estimation of
cariprazine hydrochloride in
human plasma
Mohini Shelke  +4 more
30 Apr 2024-Journal of applied
pharmaceutical research
Chat with Paper
The paper mentions that the drug Cariprazine
HCl is susceptible to Hydrolytic and Photolytic
degradation, indicating limitations in terms of
stability under certain conditions.
The Limit of Detection (LOD) for Cariprazine HCl
was found to be 0.053µg/ml, which could be
considered a limitation in terms of sensitivity
for detecting lower concentrations of the drug
in human plasma samples.
The paper does not explicitly mention any specific
research gaps that were identified during the study.
It would be beneficial for future research to explore
the stability of Cariprazine HCl in more detail under
different conditions to further understand its
degradation pathways.

66. Papers (130) Limitations Research Gap
Open access•Journal Article•DOI
(71) Bilastine - Novel anti
histamine drug for allergic rhinitis
Abhijit Trailokya  +1 more
15 Apr 2022-Indian journal of
immunology and respiratory
medicine
Chat with Paper
The paper does not specifically mention any
limitations regarding the use of Bilastine in
pediatric populations or in pregnant or
breastfeeding women. Further studies may be
needed to determine the safety and efficacy of
Bilastine in these specific patient groups.
The paper does not discuss potential drug
interactions or contraindications with other
medications that patients may be taking
concurrently with Bilastine for allergic rhinitis
or other allergic conditions. Understanding
these interactions is important for ensuring the
safe use of Bilastine in clinical practice.
The paper does not specifically address any research
gaps in the field of allergic rhinitis or the treatment of
allergic diseases with Bilastine.
There is no mention of future research directions or
areas that need further investigation in relation to the
use of Bilastine as a treatment for allergic rhinitis.

67. Papers (130) Limitations Research Gap
Journal Article•DOI
(72) Development and
Optimization of Stereoselective
Liquid Chromatographic Method
for Chiral Separation of (±)-
cetirizine and Enantiopurity
Assessment of R-levocetirizine
Valliappan Kannappan  +1 more
01 Mar 2020-Journal of Analytical
Chemistry
2 Citations
Chat with Paper
The limitations of the study were not explicitly
mentioned in the abstract provided.
Further details regarding any limitations of the
developed method or challenges faced during
the optimization process are not specified in
the text.
The paper does not explicitly mention any discussion
on the potential limitations or challenges faced during
the development and optimization of the
stereoselective liquid chromatographic method.
Addressing these research gaps could provide insights
into the practical applicability and robustness of the
method.
While the study successfully applied the developed
method for the quantitation of cetirizine enantiomers
in pharmaceutical dosage forms, there is no mention
of any future directions or recommendations for
further research in this area. Identifying potential
areas for improvement or expansion of the study
could contribute to the advancement of chiral
separation techniques.

68. Papers (130) Limitations Research Gap
Open access•Journal Article•DOI
(73) Simultaneous determination
of levocetirizine dihydrochloride
and montelukast sodium in
human plasma by LC–MS/MS:
Development, validation, and
application to a human
pharmacokinetic study
Noha S. Rashed  +1 more
01 Sep 2019-Acta
Chromatographica
2 CitationsPDF
Chat with Paper
The paper mentions that interference arising
from endogenous substances can exist in much
higher concentration than the drugs of interest,
potentially leading to high imprecision and loss
of sensitivity in the ionization of the drugs.
The study highlights the importance of
specificity in the LC-MS/MS method due to the
possibility of co-elution of endogenous
substances with the drugs of interest, which
could impact the accuracy and reliability of the
results.
The paper does not explicitly mention any specific
research gaps or limitations in the study, such as
potential challenges faced during method
development or areas for further improvement in the
analytical technique.
While the study successfully developed a sensitive and
reliable LC-MS/MS method for simultaneous
determination of levocetirizine dihydrochloride and
montelukast sodium in human plasma, it does not
discuss any future directions for expanding the
application of the method to other matrices or
potential modifications to enhance its efficiency or
sensitivity.

69. Papers (130) Limitations Research Gap
Journal Article
(74) Bioequivalence of
Levocetirizine Hydrochloride
Granules (Kangzhitai) in Healthy
Subjects.
01 May 2023
Chat with Paper
The study had a limited sample size of only
twenty healthy male subjects, which may not
fully represent the general population in terms
of demographics, health conditions, or
potential variations in drug response.
The study focused on healthy individuals, so the
findings may not be directly applicable to
individuals with underlying health conditions or
specific patient populations that may react
differently to the drugs.
The bioavailability of levocetirizine hydrochloride
granules, specifically Kangzhitai®, had not been
previously determined, indicating a research gap in
understanding the pharmacokinetics of this
formulation.
While the study confirmed the bioequivalence of
Kangzhitai® and Xyzal®, further research could explore
potential differences in efficacy or safety profiles
between the two drugs, providing valuable insights for
clinical practice.

70. Papers (130) Limitations Research Gap
Open access•Journal Article•DOI
(75) Stability indicating rp-hplc
method for estimation of
repaglinide in rabbit plasma
Raja Navamanisubramanian  +4
more
20 Apr 2019-International Journal
of Applied Pharmaceutics
PDF
Chat with Paper
The limitations mentioned in the paper include
the need for further investigation into the
potential interfering peaks in the blank
chromatograms of plasma samples and the
chromatograms of plasma spiked with both REP
and IS to ensure accurate analysis and
interpretation of results.
Another limitation highlighted is the
importance of maintaining consistency in the
percentage recovery during the liquid
extraction process, as variations in recovery
could impact the accuracy and reliability of the
method for estimating REP in rabbit plasma.
The paper does not explicitly mention any specific
research gaps or areas for further investigation, which
could limit the identification of potential gaps in the
study.
It would be beneficial for future research to address
any limitations or challenges encountered during the
development and validation of the RP-HPLC method
for estimating repaglinide in rabbit plasma, as this
could help in improving the method or expanding its
applicability.

71. Papers (130) Limitations Research Gap
Open access•Journal Article•DOI
(76) Elucidation of Inverse Agonist
Activity of Bilastine.
Hiroyuki Mizuguchi  +9 more
08 Jun 2020-Pharmaceutics
8 Citations
Chat with Paper
The paper mentions that there is no
information available to demonstrate the
inverse agonist activity of bilastine, including
inositol phosphates accumulation.
It is stated that the inverse agonist activity of
bilastine is yet to be elucidated, indicating a gap
in the current understanding of this aspect of
the drug.
The research paper highlights a gap in knowledge
regarding the inverse agonist activity of bilastine,
specifically in terms of inositol phosphates
accumulation. The paper mentions that while bilastine
has been identified as a non-sedative antihistamine
satisfying requirements for oral antihistamines, its
inverse agonist activity, including inositol phosphates
accumulation, has not been elucidated.
Another research gap identified in the paper is the
need to further investigate the potential of bilastine as
an inverse agonist. The study indicates that bilastine
demonstrated characteristics of an inverse agonist by
suppressing basal H1R gene expression in a dose-
dependent manner, suggesting a potential for
preseasonal prophylactic administration to down-
regulate basal H1R gene expression in the nasal
mucosa and alleviate nasal symptoms during peak
pollen periods.

72. Papers (130) Limitations Research Gap
Journal Article•DOI
(77) Simultaneous Determination
of Chloroquine and
Pyrimethamine with Cetirizine in
an Active Form and Human Serum
by RP-HPLC.
Hina Shamshad  +3 more
29 Oct 2021-Journal of
Chromatographic Science
9 Citations
Chat with Paper
The limitations of the study were not explicitly
mentioned in the abstract provided.
Further details regarding any limitations of the
developed RP-HPLC method for the
simultaneous determination of chloroquine,
pyrimethamine, and cetirizine in bulk drug and
human serum were not specified in the
abstract.
The paper does not mention any specific discussion on
potential limitations or challenges encountered during
the development and validation of the RP-HPLC
method for simultaneous determination of
chloroquine, pyrimethamine, and cetirizine
hydrochloride. Addressing these research gaps could
provide insights into the practical applicability and
robustness of the method.
There is no explicit mention of future directions or
areas for further research in the abstract. Including
suggestions for future studies could help in expanding
the scope of the research and exploring additional
applications or enhancements of the developed
method.

73. Papers (130) Limitations Research Gap
Open access•Journal Article•DOI
(78) Bilastine: a lifetime
companion for the treatment of
allergies
Martin K. Church  +3 more
01 Mar 2020-Current Medical
Research and Opinion
PDF
Chat with Paper
The paper does not specifically mention any
limitations related to the efficacy or safety of
bilastine in comparison to other antihistamines.
There is no discussion in the paper regarding
potential long-term side effects or interactions
of bilastine with other medications.
The paper does not specifically mention any research
gaps related to the use of bilastine in pediatric
populations, leaving a potential gap in knowledge
regarding its safety and efficacy in children.
Another research gap could be the long-term effects of
bilastine treatment beyond the currently available
data, as the paper focuses on its use as a lifetime
companion for allergies, suggesting the need for
further studies on its prolonged use.
Journal Article•DOI
(79) Development and validation
of RP-HPLC based bioanalytical
method for simultaneous
estimation of curcumin and
quercetin in rat's plasma
01 Sep 2022-South African Journal
of Botany
7 Citations
The paper mentions that the method developed
is specific for curcumin and quercetin and may
not be applicable for other compounds.
Another limitation highlighted is the need for
further studies to explore the method's
applicability in different biological matrices and
under different experimental conditions.
The paper does not mention any specific research gaps
that were identified during the study.
It is not clear from the information provided whether
the authors discussed any limitations or areas for
further research in the paper.

74. Papers (130) Limitations Research Gap
Chat with Paper
Journal Article•DOI
(80) Stability indicating RP-HPLC
method for estimation of
cariprazine hydrochloride in
human plasma
Mohini Shelke  +4 more
30 Apr 2024-Journal of applied
pharmaceutical research
Chat with Paper
The paper mentions that the drug Cariprazine
HCl is susceptible to Hydrolytic and Photolytic
degradation, indicating limitations in terms of
stability under certain conditions.
The Limit of Detection (LOD) for Cariprazine HCl
was found to be 0.053µg/ml, which could be
considered a limitation in terms of sensitivity
for detecting lower concentrations of the drug
in human plasma samples.
The paper does not explicitly mention any specific
research gaps that were identified during the study.
It would be beneficial for future research to explore
the stability of Cariprazine HCl in more detail under
different conditions to further understand its
degradation pathways.

75. Papers (130) Limitations Research Gap
Open access•Journal Article•DOI
(81) Bilastine - Novel anti
histamine drug for allergic rhinitis
Abhijit Trailokya  +1 more
15 Apr 2022-Indian journal of
immunology and respiratory
medicine
Chat with Paper
The paper does not specifically mention any
limitations regarding the use of Bilastine in
pediatric populations or in pregnant or
breastfeeding women. Further studies may be
needed to determine the safety and efficacy of
Bilastine in these specific patient groups.
The paper does not discuss potential drug
interactions or contraindications with other
medications that patients may be taking
concurrently with Bilastine for allergic rhinitis
or other allergic conditions. Understanding
these interactions is important for ensuring the
safe use of Bilastine in clinical practice.
The paper does not specifically address any research
gaps in the field of allergic rhinitis or the treatment of
allergic diseases with Bilastine.
There is no mention of future research directions or
areas that need further investigation in relation to the
use of Bilastine as a treatment for allergic rhinitis.

76. Papers (130) Limitations Research Gap
Journal Article•DOI
(82) Development and
Optimization of Stereoselective
Liquid Chromatographic Method
for Chiral Separation of (±)-
cetirizine and Enantiopurity
Assessment of R-levocetirizine
Valliappan Kannappan  +1 more
01 Mar 2020-Journal of Analytical
Chemistry
2 Citations
Chat with Paper
The limitations of the study were not explicitly
mentioned in the abstract provided.
Further details regarding any limitations of the
developed method or challenges faced during
the optimization process are not specified in
the text.
The paper does not explicitly mention any discussion
on the potential limitations or challenges faced during
the development and optimization of the
stereoselective liquid chromatographic method.
Addressing these research gaps could provide insights
into the practical applicability and robustness of the
method.
While the study successfully applied the developed
method for the quantitation of cetirizine enantiomers
in pharmaceutical dosage forms, there is no mention
of any future directions or recommendations for
further research in this area. Identifying potential
areas for improvement or expansion of the study
could contribute to the advancement of chiral
separation techniques.

77. Papers (130) Limitations Research Gap
Open access•Journal Article•DOI
(83) Simultaneous determination
of levocetirizine dihydrochloride
and montelukast sodium in
human plasma by LC–MS/MS:
Development, validation, and
application to a human
pharmacokinetic study
Noha S. Rashed  +1 more
01 Sep 2019-Acta
Chromatographica
2 CitationsPDF
Chat with Paper
The paper mentions that interference arising
from endogenous substances can exist in much
higher concentration than the drugs of interest,
potentially leading to high imprecision and loss
of sensitivity in the ionization of the drugs.
The study highlights the importance of
specificity in the LC-MS/MS method due to the
possibility of co-elution of endogenous
substances with the drugs of interest, which
could impact the accuracy and reliability of the
results.
The paper does not explicitly mention any specific
research gaps or limitations in the study, such as
potential challenges faced during method
development or areas for further improvement in the
analytical technique.
While the study successfully developed a sensitive and
reliable LC-MS/MS method for simultaneous
determination of levocetirizine dihydrochloride and
montelukast sodium in human plasma, it does not
discuss any future directions for expanding the
application of the method to other matrices or
potential modifications to enhance its efficiency or
sensitivity.

78. Papers (130) Limitations Research Gap
Journal Article
(84) Bioequivalence of
Levocetirizine Hydrochloride
Granules (Kangzhitai) in Healthy
Subjects.
01 May 2023
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The study had a limited sample size of only
twenty healthy male subjects, which may not
fully represent the general population in terms
of demographics, health conditions, or
potential variations in drug response.
The study focused on healthy individuals, so the
findings may not be directly applicable to
individuals with underlying health conditions or
specific patient populations that may react
differently to the drugs.
The bioavailability of levocetirizine hydrochloride
granules, specifically Kangzhitai®, had not been
previously determined, indicating a research gap in
understanding the pharmacokinetics of this
formulation.
While the study confirmed the bioequivalence of
Kangzhitai® and Xyzal®, further research could explore
potential differences in efficacy or safety profiles
between the two drugs, providing valuable insights for
clinical practice.

79. Papers (130) Limitations Research Gap
Open access•Journal Article•DOI
(85) Stability indicating rp-hplc
method for estimation of
repaglinide in rabbit plasma
Raja Navamanisubramanian  +4
more
20 Apr 2019-International Journal
of Applied Pharmaceutics
PDF
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The limitations mentioned in the paper include
the need for further investigation into the
potential interfering peaks in the blank
chromatograms of plasma samples and the
chromatograms of plasma spiked with both REP
and IS to ensure accurate analysis and
interpretation of results.
Another limitation highlighted is the
importance of maintaining consistency in the
percentage recovery during the liquid
extraction process, as variations in recovery
could impact the accuracy and reliability of the
method for estimating REP in rabbit plasma.
The paper does not explicitly mention any specific
research gaps or areas for further investigation, which
could limit the identification of potential gaps in the
study.
It would be beneficial for future research to address
any limitations or challenges encountered during the
development and validation of the RP-HPLC method
for estimating repaglinide in rabbit plasma, as this
could help in improving the method or expanding its
applicability.

80. Papers (130) Limitations Research Gap
Open access•Journal Article•DOI
(86) Elucidation of Inverse Agonist
Activity of Bilastine.
Hiroyuki Mizuguchi  +9 more
08 Jun 2020-Pharmaceutics
8 Citations
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The paper mentions that there is no
information available to demonstrate the
inverse agonist activity of bilastine, including
inositol phosphates accumulation.
It is stated that the inverse agonist activity of
bilastine is yet to be elucidated, indicating a gap
in the current understanding of this aspect of
the drug.
The research paper highlights a gap in knowledge
regarding the inverse agonist activity of bilastine,
specifically in terms of inositol phosphates
accumulation. The paper mentions that while bilastine
has been identified as a non-sedative antihistamine
satisfying requirements for oral antihistamines, its
inverse agonist activity, including inositol phosphates
accumulation, has not been elucidated.
Another research gap identified in the paper is the
need to further investigate the potential of bilastine as
an inverse agonist. The study indicates that bilastine
demonstrated characteristics of an inverse agonist by
suppressing basal H1R gene expression in a dose-
dependent manner, suggesting a potential for
preseasonal prophylactic administration to down-
regulate basal H1R gene expression in the nasal
mucosa and alleviate nasal symptoms during peak
pollen periods.

81. Papers (130) Limitations Research Gap
Journal Article•DOI
(87) Simultaneous Determination
of Chloroquine and
Pyrimethamine with Cetirizine in
an Active Form and Human Serum
by RP-HPLC.
Hina Shamshad  +3 more
29 Oct 2021-Journal of
Chromatographic Science
9 Citations
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The limitations of the study were not explicitly
mentioned in the abstract provided.
Further details regarding any limitations of the
developed RP-HPLC method for the
simultaneous determination of chloroquine,
pyrimethamine, and cetirizine in bulk drug and
human serum were not specified in the
abstract.
The paper does not mention any specific discussion on
potential limitations or challenges encountered during
the development and validation of the RP-HPLC
method for simultaneous determination of
chloroquine, pyrimethamine, and cetirizine
hydrochloride. Addressing these research gaps could
provide insights into the practical applicability and
robustness of the method.
There is no explicit mention of future directions or
areas for further research in the abstract. Including
suggestions for future studies could help in expanding
the scope of the research and exploring additional
applications or enhancements of the developed
method.

82. Papers (130) Limitations Research Gap
Open access•Journal Article•DOI
(88) Bilastine: a lifetime
companion for the treatment of
allergies
Martin K. Church  +3 more
01 Mar 2020-Current Medical
Research and Opinion
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The paper does not specifically mention any
limitations related to the efficacy or safety of
bilastine in comparison to other antihistamines.
There is no discussion in the paper regarding
potential long-term side effects or interactions
of bilastine with other medications.
The paper does not specifically mention any research
gaps related to the use of bilastine in pediatric
populations, leaving a potential gap in knowledge
regarding its safety and efficacy in children.
Another research gap could be the long-term effects of
bilastine treatment beyond the currently available
data, as the paper focuses on its use as a lifetime
companion for allergies, suggesting the need for
further studies on its prolonged use.
Journal Article•DOI
(89) Development and validation
of RP-HPLC based bioanalytical
method for simultaneous
estimation of curcumin and
quercetin in rat's plasma
01 Sep 2022-South African Journal
of Botany
7 Citations
The paper mentions that the method developed
is specific for curcumin and quercetin and may
not be applicable for other compounds.
Another limitation highlighted is the need for
further studies to explore the method's
applicability in different biological matrices and
under different experimental conditions.
The paper does not mention any specific research gaps
that were identified during the study.
It is not clear from the information provided whether
the authors discussed any limitations or areas for
further research in the paper.

83. Papers (130) Limitations Research Gap
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Journal Article•DOI
(90) Stability indicating RP-HPLC
method for estimation of
cariprazine hydrochloride in
human plasma
Mohini Shelke  +4 more
30 Apr 2024-Journal of applied
pharmaceutical research
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The paper mentions that the drug Cariprazine
HCl is susceptible to Hydrolytic and Photolytic
degradation, indicating limitations in terms of
stability under certain conditions.
The Limit of Detection (LOD) for Cariprazine HCl
was found to be 0.053µg/ml, which could be
considered a limitation in terms of sensitivity
for detecting lower concentrations of the drug
in human plasma samples.
The paper does not explicitly mention any specific
research gaps that were identified during the study.
It would be beneficial for future research to explore
the stability of Cariprazine HCl in more detail under
different conditions to further understand its
degradation pathways.

84. Papers (130) Limitations Research Gap
Journal Article
(91) Bioequivalence of
Levocetirizine Hydrochloride
Granules (Kangzhitai) in Healthy
Subjects.
Hong Xiang  +2 more
23 Mar 2023-Alternative
Therapies in Health and Medicine
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The study had a small sample size of only
twenty healthy male subjects, which may limit
the generalizability of the findings to a broader
population.
The bioavailability of levocetirizine
hydrochloride granules was only examined in
healthy individuals, so the results may not
directly apply to individuals with specific health
conditions or diseases.
The study focused on healthy male subjects only, so
there is a research gap in terms of generalizing the
findings to a more diverse population that includes
females and individuals with underlying health
conditions.
The research did not investigate potential differences
in bioavailability based on age groups, so there is a
gap in understanding how age may impact the
bioequivalence of Kangzhitai® and Xyzal®.

85. Papers (130) Limitations Research Gap
Journal Article•DOI
(92) Development and validation
of RP-HPLC method for estimation
of fisetin in rat plasma
Rajan Kumar  +10 more
01 Aug 2021-South African
Journal of Botany
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The paper mentions that the developed RP-
HPLC method for estimation of fisetin in rat
plasma has a limitation in terms of its
sensitivity, as the method was not sensitive
enough to detect lower concentrations of
fisetin in the plasma samples.
Another limitation highlighted in the paper is
the lack of investigation into the stability of
fisetin in rat plasma under different storage
conditions, which could have provided valuable
insights into the reliability of the method over
time.
The paper does not mention any specific research gaps
that were identified during the study.
It is important for future research to explore potential
limitations or areas for improvement in the
development and validation of RP-HPLC method for
estimation of fisetin in rat plasma.

86. Papers (130) Limitations Research Gap
Journal Article•DOI
(93) Bioequivalence Evaluation of
Three Pediatric Oral Formulations
of Bilastine in Healthy Subjects:
Results from a Randomized, Open
Label, Crossover Study
Belén Sádaba  +5 more
01 Apr 2020-European Journal of
Drug Metabolism and
Pharmacokinetics
4 Citations
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The study sample size was estimated based on
a coefficient of variability for bilastine from
previous studies, which may introduce
limitations in terms of generalizability to larger
populations or specific subgroups.
The study focused on healthy adult volunteers,
which may limit the generalizability of the
findings to pediatric populations or individuals
with specific health conditions that could affect
bilastine pharmacokinetics.
The study focused on healthy adult volunteers, but did
not include pediatric subjects, which are the target
population for the pediatric oral formulations of
bilastine. Including pediatric subjects in the study
could provide more comprehensive data on the
bioequivalence and tolerability of the formulations in
the intended patient population.
The study did not investigate the potential impact of
factors such as age, weight, or specific medical
conditions on the pharmacokinetics and tolerability of
the pediatric oral formulations of bilastine.
Understanding how these factors may influence the
efficacy and safety of the formulations could help
optimize dosing strategies for different pediatric
patients.

87. Papers (130) Limitations Research Gap
Open access•DOI
(94) Simultaneous determination
of levocetrizine and
phenylpropanolamine
hydrocholride by RP-HPLC
A. Srinivasan  +3 more
17 Oct 2020
PDF
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The limitations of the study include the lack of
discussion on potential interferences from
excipients used in the formulation, which could
affect the accuracy and precision of the
proposed RP-HPLC method.
Another limitation is the absence of detailed
information on the stability of the analytes in
the sample solutions over time, which is crucial
for ensuring the reliability of the results
obtained from the simultaneous determination
of levocetirizine and phenylpropanolamine HCl
in solid dosage forms.
The paper does not explicitly mention any discussion
or exploration of potential limitations or challenges
faced during the development and validation of the
RP-HPLC method for the simultaneous determination
of levocetirizine and phenylpropanolamine HCl.
Providing insights into any difficulties encountered or
areas for improvement could have added depth to the
research.
While the paper focuses on the simultaneous
estimation of levocetirizine and phenylpropanolamine
HCl in solid dosage forms, it does not delve into the
potential application of the developed method in
analyzing these compounds in biological samples or in
vivo studies. Exploring the feasibility and challenges of
applying the method in biological matrices could have
been a valuable addition to the research.

88. Papers (130) Limitations Research Gap
Open access•Journal Article•DOI
(95) Bilastine in pruritus
associated with dermatological
conditions
Abhay Talathi  +5 more
01 Jul 2022-Indian journal of
drugs in dermatology
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The study design was an open-label study,
which may introduce bias in the results as both
the patients and the researchers were aware of
the treatment being administered.
The study duration was limited to 4 weeks,
which may not capture the long-term
effectiveness and safety profile of bilastine in
managing pruritus associated with
dermatological conditions.
Limited number of studies demonstrating the
effectiveness and safety of bilastine in managing
pruritus associated with various skin diseases,
highlighting the need for more research in this area.
Lack of specific data on the long-term effects and
outcomes of using bilastine for pruritus associated
with dermatological conditions, indicating a gap in
knowledge regarding the sustained efficacy and safety
profile of the medication.

89. Papers (130) Limitations Research Gap
Open access•Journal Article•DOI
(96) Bioanalytical method for
teriflunomide estimation by hplc
Pooja Sharma Arun M Gurav  +1
more
07 Jan 2022-Asian Journal of
Pharmaceutical and Clinical
Research
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The limitations of the study were not explicitly
mentioned in the abstract provided.
Further details regarding any limitations of the
developed HPLC method for teriflunomide
estimation were not specified in the abstract.
The paper does not mention any discussion on
potential limitations or challenges faced during the
development and validation of the HPLC method for
teriflunomide estimation. Addressing these aspects
could provide insights into areas for further research
or improvement.
There is no mention of comparisons with existing
methods or techniques for teriflunomide estimation in
human plasma. Conducting comparative studies could
help identify the advantages or disadvantages of the
developed method and highlight areas where further
research may be needed.

90. Papers (130) Limitations Research Gap
Journal Article•DOI
(97) Pharmacokinetics and Safety
of Bilastine 10 mg/d in Children
Aged 2 to 5 Years With Allergic
Rhinoconjunctivitis or Urticaria: A
Phase 3 Clinical Trial.
B. Majorek-Olechowska  +6 more
09 May 2024-Journal of
Investigational Allergology and
Clinical Immunology
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The study had a relatively small sample size,
with a total of 37 children included, which may
limit the generalizability of the findings to a
larger population.
The safety evaluation focused on treatment-
emergent adverse events (TEAEs), vital signs,
and physical examination, but other potential
safety concerns or long-term effects may not
have been fully explored in this study.
The study focused on the pharmacokinetics and safety
of oral bilastine 10 mg/d in children aged 2 to 5 years
with allergic rhinoconjunctivitis or urticaria, but did
not explore the efficacy of the medication in managing
these conditions in this specific age group.
While the safety profile of bilastine was assessed, the
study did not delve into potential long-term effects or
interactions with other medications commonly used in
children aged 2 to 5 years.

91. Papers (130) Limitations Research Gap
Open access•Journal Article•DOI
(98) Optimization and Validation
of a novel Nebulizer-assisted
Liquid Phase Microextraction
Followed by HPLC-DAD for
Diazinon Analysis in Plasma
Samples
Reza Mohammadzaheri  +3 more
01 Oct 2019-International journal
of medical toxicology and forensic
medicine
PDF
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The limitations mentioned in the paper include
the negative impact of sonication, type, and
volume of dispersive solvents on maximum
recovery, which affected the extraction
efficiency.
Another limitation highlighted is the need for
further optimization of parameters such as
surfactant, salt amount, and pH to enhance the
extraction and determination of diazinon in
plasma samples using the NALPME-HPLC-DAD
method.
The paper does not explicitly mention any specific
research gaps identified within the study. It focuses on
the optimization and validation of a novel method for
analyzing diazinon in plasma samples, highlighting the
method's simplicity, accuracy, precision, and
sensitivity.
While the study successfully presents a new approach
for diazinon analysis, potential research gaps could
include a discussion on the limitations of the method,
comparison with existing techniques, or suggestions
for future research directions to further enhance the
applicability and efficiency of the proposed method.

92. Papers (130) Limitations Research Gap
Open access•DOI
(99) Reverse Phase HPLC Method
for the Simultaneous
Determination of Cetirizine,
Verapamil/Diltiazem and
Amlodipine
Hina Shamshad  +2 more
01 Apr 2021
3 CitationsPDF
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The method validation in the paper was based
on accuracy, precision (%RSD>2), and linearity
(>0.999), but other parameters like robustness
and ruggedness were not mentioned as part of
the validation process.
While the method specificity was reported to
be good with no interference of excipients from
tablets observed, potential interference from
other compounds in biological samples was not
addressed in the limitations section of the
paper.
The paper does not mention any discussion on the
potential limitations or challenges faced during the
development and validation of the RP-HPLC method,
which could be considered a research gap.
There is no indication in the abstract of the paper
regarding any comparison with existing methods or
techniques for the simultaneous determination of the
mentioned drugs, which could be another research
gap.

93. Papers (130) Limitations Research Gap
Patent
(100) Detection method of
cetirizine hydrochloride related
substances
Zhang Guimin  +2 more
27 Oct 2020
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The detection method described in the paper
utilizes a C18 column, which may limit the
analysis to compounds that can be effectively
separated using this specific type of column.
The detection wavelength range is specified to
be between 190 nm and 260 nm, which may
restrict the detection of substances that absorb
outside of this range.
The paper does not specifically mention any identified
research gaps within the field of pharmaceutical
analysis related to cetirizine hydrochloride and its
related substances.
The focus of the paper is on presenting a detection
method using high-performance liquid
chromatography for cetirizine hydrochloride related
substances, highlighting its sensitivity, resolution,
repeatability, and reliability, rather than discussing
research gaps.
Open access•Journal Article•DOI
(101) Bioanalytical RP-HPLC
method development and
validation for estimation of
azelnidipine and Olmesartan
medoxomil in human plasma
Anuja P. Bhosale
31 Oct 2022-Journal of medical
pharmaceutical and allied
The limitations of the study were not explicitly
mentioned in the abstract provided.
Further details on the limitations of the
developed RP-HPLC method for estimation of
Azelnidipine and Olmesartan medoxomil in
human plasma were not specified in the
abstract.
The paper does not mention any specific discussion or
identification of research gaps within the study.
It would be beneficial for future research to explore
any limitations or areas for improvement in the
developed RP-HPLC method for estimation of
Azelnidipine and Olmesartan medoxomil in human
plasma.

94. Papers (130) Limitations Research Gap
sciences
PDF
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Journal Article•DOI
(102) Fast simultaneous
quantification of gabapentin and
cetirizine in cell lysates by means
of HPLC-MS/MS.
Lukas Schlatt  +4 more
30 May 2020-Journal of
Pharmaceutical and Biomedical
Analysis
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The study mentions that the method developed
is specific for gabapentin and cetirizine and may
not be applicable for other drugs.
The authors note that the method may require
further validation for use in different biological
matrices due to potential matrix effects.
The paper does not mention any specific research gaps
or limitations in the study.
It does not provide any suggestions for future research
directions or areas that need further investigation.

95. Papers (130) Limitations Research Gap
Journal Article•DOI
(103) Advanced Derivative
Spectroscopic Method for
Estimation of Montelukast and
Bilastine in Their Tablet Dosage
Form
Kinjal Detroja  +1 more
29 Mar 2020-International Journal
of Pharmaceutical Sciences and
Drug Research
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- The paper does not mention any discussion or
exploration of potential interferences or co-eluting
peaks that could affect the accurate estimation of
Montelukast and Bilastine in their tablet dosage form.
Further research could focus on identifying and
addressing any such potential interferences.
The paper does not elaborate on the comparison of
the developed advanced derivative spectroscopic
method with other existing methods for the
estimation of Montelukast and Bilastine. Future
studies could include a comparative analysis to
evaluate the advantages and limitations of this new
method in relation to traditional techniques.

96. Papers (130) Limitations Research Gap
Journal Article•DOI
(104) Novel bio-analytical
technique for estimation of
gamma oryzanol in rat plasma
and brain homogenate using
HPLC.
Surya Pratap Singh  +3 more
15 Jul 2020-Annales
pharmaceutiques françaises
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The limitations of the study were not explicitly
mentioned in the abstract provided.
Further details regarding any limitations of the
novel bio-analytical technique for estimation of
gamma oryzanol in rat plasma and brain
homogenate would require a thorough reading
of the full paper.
The paper does not mention any discussion on the
potential limitations or challenges faced during the
development of the bioanalytical method for
estimating gamma oryzanol in rat plasma and brain
homogenate. Addressing these limitations could
provide insights into areas for further improvement or
refinement of the technique.
There is no mention of comparisons with existing
methods or techniques for estimating gamma oryzanol
in rat plasma and brain homogenate. Including a
discussion on the advantages or disadvantages of this
novel bio-analytical technique compared to traditional
methods could help in identifying the unique
contributions and potential advancements in the field.

97. Papers (130) Limitations Research Gap
Journal Article•DOI
(105) Separation and
identification of cetirizine
enantiomers in human urine by
CE and circular dichroism
independent of their standards.
Lixia Chen  +5 more
09 Feb 2023-Journal of Separation
Science
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The study focused on cetirizine enantiomers
and their identification in human urine, limiting
the generalizability of the method to other
chiral drugs.
The method relied on the use of electronic
circular dichroism for identification, which may
require specialized equipment and expertise,
potentially limiting its widespread application
in laboratories without access to such
resources.
The paper does not mention the potential limitations
or challenges faced during the separation and
identification process of cetirizine enantiomers using
CE and circular dichroism. Addressing these issues
could provide insights into improving the
methodology.
Further exploration into the applicability of the
developed method for other chiral drugs beyond
cetirizine could be beneficial to assess its versatility
and potential for broader pharmaceutical applications.

98. Papers (130) Limitations Research Gap
Journal Article•DOI
(106) Rapid RP-HPLC Method for
Simultaneous estimation of
Umeclidinium and Vilanterol in
human plasma
G. Raveendra Babu  +6 more
31 Mar 2023-Research journal of
pharmacy and technology
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The limitations of the study were not explicitly
mentioned in the abstract provided.
Therefore, specific limitations such as
challenges faced during method development,
potential sources of error, or constraints in the
experimental design are not outlined in the
text.
The paper does not mention any discussion on the
potential limitations or challenges faced during the
development and validation of the RP-HPLC method
for the simultaneous estimation of Umeclidinium and
Vilanterol in human plasma. Providing insights into
these aspects could have added depth to the research.
There is no mention of any comparison with existing
methods or techniques for the estimation of
Umeclidinium and Vilanterol in human plasma.
Including a discussion on the advantages or
improvements of the proposed method over previous
approaches could have highlighted the novelty and
significance of the research.

99. Papers (130) Limitations Research Gap
Journal Article•DOI
(107) Pharmacokinetics of
hydroxyzine and cetirizine
following oral administration of
hydroxyzine to exercised
Thoroughbred horses.
Heather K Knych  +4 more
06 Sep 2019-Journal of Veterinary
Pharmacology and Therapeutics
2 Citations
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The study highlights the lack of published
studies suitable for establishing appropriate
regulatory recommendations regarding the use
of hydroxyzine in performance horses,
indicating a gap in knowledge in this area.
The findings suggest that a prolonged
withdrawal time should be observed if
hydroxyzine is used in performance horses, as it
is classified as a prohibited substance by the
applicable regulatory body, emphasizing the
need for careful consideration and monitoring
of its administration in such contexts.
Lack of published studies establishing appropriate
regulatory recommendations for the use of
hydroxyzine in performance horses, despite its
common usage.
The need for a prolonged withdrawal time for
hydroxyzine if administered to performance horses, as
it is classified as a prohibited substance by the
applicable regulatory body.

100. Papers (130) Limitations Research Gap
Open access•Journal Article•DOI
(108) Rapid RP-HPLC method for
simultaneous estimation of
metformin, pioglitazone, and
glimepiride in human plasma
Mahmoud M. Sebaiy  +3 more
01 Mar 2020-Acta
Chromatographica
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The limitations of the paper were not explicitly
mentioned in the provided text.
Therefore, specific limitations of the developed
RP-HPLC method for simultaneous estimation
of metformin, pioglitazone, and glimepiride in
human plasma were not outlined in the text.
The paper highlights the lack of previous studies
utilizing chromatographic techniques in biological
samples for the simultaneous estimation of
metformin, pioglitazone, and glimepiride, despite their
synergistic action in treating diabetes mellitus type II.
This gap in research emphasizes the novelty and
importance of the developed RP-HPLC method
presented in the study.
Another research gap addressed in the paper is the
need for a rapid, reproducible, and sensitive
chromatographic method that can protect analytical
scientists and chemists from exposure to volatile and
corrosive organic solvents during experimentation.
The environmentally benign mobile phase used in the
developed method addresses this gap and provides a
safer alternative for conducting analytical procedures.
Open access•Journal Article•DOI The paper mentions that one of the limitations
is the lack of investigation into the stability of
The paper does not mention any specific research gaps
that were identified or discussed in relation to the

101. Papers (130) Limitations Research Gap
(109) Quantitative estimation of
cilnidipine and valsartan in rat
plasma by RP-HPLC: its
pharmacokinetic application
Ramanlal N. Kachave  +2 more
01 Dec 2021-Future Journal of
Pharmaceutical Sciences
4 CitationsPDF
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cilnidipine and valsartan in rat plasma under
different storage conditions.
Another limitation highlighted in the paper is
the absence of a comparison with other
analytical methods for the quantification of
cilnidipine and valsartan in rat plasma.
study on the quantitative estimation of cilnidipine and
valsartan in rat plasma by RP-HPLC.
Without explicit mention of research gaps, it is unclear
what areas of the study could be further explored or
improved upon in future research.

102. Papers (130) Limitations Research Gap
Open access•Journal Article•DOI
(110) Bilastine in higher doses in
chronic spontaneous urticaria
Kiran Godse  +1 more
28 Mar 2021
PDF
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The study had a small sample size of 30
patients, which may limit the generalizability of
the findings to a larger population.
Three patients were lost to follow-up after one
week of treatment, which could potentially
affect the accuracy of the results and
conclusions drawn from the study.
The study did not include a control group receiving a
placebo or standard dose of bilastine for comparison,
which could have provided more robust evidence on
the efficacy of higher doses.
The long-term effects and safety profile of using
bilastine in doses higher than the standard
recommended dose were not extensively studied,
indicating a need for further research on the potential
risks associated with prolonged use of elevated doses.
Open access•Journal Article•DOI
(111) Bioanalytical RP-HPLC
method development and
validation for estimation of
azelnidipine and Olmesartan
medoxomil in human plasma
Anuja P. Bhosale
31 Oct 2022-Journal of medical
The limitations of the study were not explicitly
mentioned in the abstract provided.
Further details on the limitations of the
developed RP-HPLC method for estimation of
Azelnidipine and Olmesartan medoxomil in
human plasma were not specified in the
abstract.
The paper does not mention any specific discussion or
identification of research gaps within the study.
It would be beneficial for future research to explore
any limitations or areas for improvement in the
developed RP-HPLC method for estimation of
Azelnidipine and Olmesartan medoxomil in human
plasma.

103. Papers (130) Limitations Research Gap
pharmaceutical and allied
sciences
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Journal Article•DOI
(112) Fast simultaneous
quantification of gabapentin and
cetirizine in cell lysates by means
of HPLC-MS/MS.
Lukas Schlatt  +4 more
30 May 2020-Journal of
Pharmaceutical and Biomedical
Analysis
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The study mentions that the method developed
is specific for gabapentin and cetirizine and may
not be applicable for other drugs.
The authors note that the method may require
further validation for use in different biological
matrices due to potential matrix effects.
The paper does not mention any specific research gaps
or limitations in the study.
It does not provide any suggestions for future research
directions or areas that need further investigation.