Biosafety-and-Biosecurity-in-Molbio.pptx

biosecurity and biosafety

1. LABORATORY BIOSAFETY AND BIOSECURITY Ms. Junalen Aurelio-Dadivas, RMT, MSMT (CAR)

2. BIOSAFETY “the containment principles, technologies and practices that are implemented to prevent unintentional exposure to pathogens, toxins and their accidental release”. BIOSECURITY “refers to the protection, control and accountability for valuable biological materials within laboratories in order to prevent their unauthorized access, loss, theft, misuse, diversion or intentional release”.

3. CLASSIFICATIONS OF MICROORGANISMS ACCORDING TO RISK GROUP based on: Agent’s pathogenicity mode of transmission host range availability of preventative measures & effective treatment. Infective microorganisms: Risk group 1 Risk group 2 Risk group 3 Risk group 4

4. CLASSIFICATIONS OF MICROORGANISMS ACCORDING TO RISK GROUP RISK GROUP 1 includes microorganisms that are unlikely to cause human or animal disease. These microorganisms bring about low individual and community risk. RISK GROUP 2 includes microorganisms that are unlikelyto be significant risk to laboratory workers & community, livestock or the environment. Lab exposure may cause infection however effective treatment measures are available. Brings out moderate individual risk and limited community risk.

5. CLASSIFICATIONS OF MICROORGANISMS ACCORDING TO RISK GROUP RISK GROUP 3 includes microorganisms that are known to cause serious diseasesto humans or animals and may a significant risk to lab workers. Brings out high individual risk and limited to moderate community risk. RISK GROUP 4 includes microorganisms that are known to produce life-threatening diseases to humans or animals. It represents a significant risk to lab workers and may be readily transmissible from one individual to another while effective and preventive measures are not usually available. Brings out high individual and community risk .

6. LABORATORY BIOSAFETY LEVELS BIOSAFETY LEVEL 1 (BSL-1) suitable for work involving viable microorganisms that are defined & well characterized strains known not to cause disease in humans. (Bacillus subtilis, Naegleria gruberi) this is appropriate for undergraduate and secondary educational training & teaching lab w/c requires basic lab practices, safety equipment, facility design and level,of containment.

7. LABORATORY BIOSAFETY LEVELS BIOSAFETY LEVEL 2 (BSL-2) designed for laboratories that pose a moderate potential hazards for lab workers and environment. (Hepatitis B virus, HIV, Salmonella, Toxoplasma species) this is appropriate when work done w/ human blood, body fluids, tissues. Access to the lab must be restricted when work up is conducted.

8. LABORATORY BIOSAFETY LEVELS BIOSAFETY LEVEL 3 (BSL-3) designed for protection of workers both primary and secondary barriers. exposure to inigenous and exotic agents with a potential for respiratory transmission may cause serious and lethal infections. (Mycobacterium tuberculosis, St. Louis encephalitis virus and Coxiella.)

9. LABORATORY BIOSAFETY LEVELS BIOSAFETY LEVEL 3 (BSL-3) designed for work with dangerous and exotic agents that pose high individual risks of life threatening diseases that may be transmitted via aerosol route for w/c there NO available vaccines or treatment. Agents like Marburg or Crimean Congo hemorrhagic fever Class III biosafety cabinet is needed and with positive pressure.

10. Fume hood VS Biosafety Cabinet (BSC)

11. BSC classes The class I biological safety hood uses an exhaust fan to move air inward through the open front. The air is circulated within the safety hood, passing through a high-efficiency particulate air (HEPA) filter before reaching the environment outside the hood.

12. BSC classes The class II biological safety hood is the most common in microbiology laboratories. Air is pulled inward and downward by a blower and passed up through the airflow plenum where it passes through a HEPA filter before reaching the work surface. A percentage of the remaining air is HEPA filtered before reaching the environment.

13. BSC classes The class III biological safety hood is a self-contained ventilated system for highly infectious microorganisms or materials and provides the highest level of personal protection. The closed front contains attached gloves for manipulation on the work surface.