BIOSYNTESIS OF CYTOKININS AND GIBBERALINS

This presentation will provide a description about the biosynthesis of plant growth regulator- Cytokinins and gibberalins and their role in plant growth and development

1. BIOSYNTHESIS AND
FUNCTIONS OF CYTOKININS
AND GIBBERALINS:
DR. GURPREET SINGH, Ph.D
Assistant Professor
Post-Graduate Department of Biotechnology
Lyallpur Khalsa College, Jalandhar (INDIA)
EMAIL: [email protected]

2. CarlosMilleretalin1955isolateda
“cell-division-stimulatingfactor”from
yeastDNA.
Itwasnamedaskinetinbecauseofits
amazingpowertostimulatecell
division(cytokinesis)inthepresenceof
anauxin.

6. CYTOKININS (Natural occurring)

9. methylerythritolphosphate (MEP
mevalonate (MVA) pathway

12. Biosynthesis of Cytokinin
•Thefirstcommittedstepincytokininbiosynthesisistheadditionof
theisopentenylsidechainfromDMAPPtoanadenosinemoiety.
•TheplantandbacterialIPTenzymesdifferintheadenosinesubstrate
used;theplantenzymeappearstoutilizebothADPandATP,andthe
bacterialenzymeutilizesAMP.
•Theproductsofthesereactions(iPMP,iPDP,oriPTP)areconvertedto
zeatinbyanunidentifiedhydroxylase.

13. •Thefirstcommittedstepincytokininbiosynthesisisthetransferof
theisopentenylgroupofdimphate(DMAPP)toanadenosinemoiety.
•Inbothcases,DMAPPandAMPareconvertedto
isopentenyladenosine-5′-monophosphate(iPMP).
•Aswiththefreecytokinins,isopentenylgroupsaretransferredtothe
adeninemoleculesfromDMAPPbyanenzymecalledtRNA-IPT.
Biosynthesis of Cytokinin

14. CytokininBiosynthesis
Biosynthetic pathway for cytokinin biosynthesis. The first committed step in cytokinin biosynthesis
is the addition of the isopentenyl side chain from DMAPP to an adenosine moiety. The plant and
bacterial IPT enzymes differ in the adenosine substrate used; the plant enzyme appears to utilize
both ADP and ATP, and the bacterial enzyme utilizes AMP. The products of these reactions (iPMP,
iPDP, or iPTP) are converted to zeatin by an unidentified hydroxylase. The various phosphorylated
forms can be interconverted and free trans-Zeatin can be formed from the riboside by enzymes of
general purine metabolism. trans-Zeatin can be metabolized in various ways as shown, and these
reactions are catalyzed by the indicated enzymes.

25. GIBBERALINS
Gibberellins are tetracyclic diterpenoidsmade up of four isoprenoid units.
Terpenoidsare compounds made up of five-carbon (isoprene) building blocks: joined head to tail.
the

29. STAGEA-whichoccursin
plastids,leadstothesynthesis
ofthetetracyclichydrocarbon,
ent-kaurene
STAGEB-whichoccursinthe
endoplasmicreticulum,ent-
kaureneissequentiallyoxidized
toyieldthefirstformedGA,
GA12andits13-hydroxylated
analogGA53.
STAGEC-whichoccursinthe
cytosol,GA12andGA53are
furtheroxidizedtootherC20-
GAs,andC19-GAs.

31. Buchanan, B et al., Biochemistry and Molecular Biology of Plants; chapter 17