Blood component separation

BLOOD COMPONENT SEPARATION

1. Blood components & Preparation Dr . T. Arivazhagan Post Graduate Department Of Pathology

2. Need for separation

3. Single whole blood contain Red cell Platelets Cryoprecipitate Frozen plasma

4. Uses of whole blood Massive transfusion to correct acute hypovolemic state in trauma and shock Now a days rarely used because of volume overload

5. Coombs test Also known as antiglobulin test To find out the presence of antibodies Direct Indirect

6. Direct comb’s test   Used to detect the antibodies or complement proteins Blood sample is taken & the RBCs are washed (removing the patient's own plasma) Then incubated with anti-human globulin (also known as "Coombs reagent "). Agglutination present – Positive –Presence of Antibodies

7. Indirect coombs test   Used in prenatal testing of pregnant women Serum is extracted from the blood sample Serum is incubated with RBCs of known  antigenicity   Anti-human globulin is added Agglutination occurs , the indirect Coombs test is positive