Bvaaj aaj aaj aaj aaj aaj lid (28)-1.pptx

Yes

1. Topic : Types of protein engineering Submitted to: Dr M.Kabir Submitted by: Hamza Khalid Roll No: BOTN51BF20R028 Semester: Botany 7th Session: 2020-2024 Department: Department of Biological Sciences Institute: Thal University Bhakkar 1 1

2. Contents: Types of protein engineering Site Directed Mutagensis Directed evolution Synthetic biology 2

3. 1-Site directed mutagenesis: Site-directed mutagenesis is a technique in protein engineering that allows for the specific modification of a protein’s DNA sequence at a defined site. This technique involves introducing a desired mutation into the DNA sequence that encodes a protein, resulting in a modified protein with altered structure and/or function. Site-directed mutagenesis is a powerful tool for understanding the relationship between protein structure and function, and for creating proteins with improved properties for various applications in biotechnology, medicine, and industry 3

4. Steps involved: Design of the desired mutation.
Generation of the mutated DNA sequences. Cloning of the mutated DNA sequences.
Expression of the mutated protein.
Characterization of the mutated protein. 4

5. Applications: Biomedical research Drug development Biocatalysis Structural biology Protein engineering Genetic engineering 5

6. 2-Directed evolution: Directed evolution is a method of protein engineering that mimics natural selection. By exposing a protein to random mutations and selecting for improved variants. Following steps are involved in the directed evolution: 6

7. Steps involved: Generation of a mutant library Screening or selection Amplifications and repeatition Characterization 7

8. 3-Synthetic biology: Synthetic biology is a field of biology that aims to design and construct novel biological systems for practical purposes. It involves the manipulation and engineering of biological components, such as DNA, cells, and organisms, to create new functions and systems that do not exist in nature.
Synthetic biology has applications in areas such as medicine, biotechnology, and bioremediation. It also has the potential to revolutionize the way we approach problems in these fields by providing different tools. 8

9. Applications: Medicinal applications Biotechnological application Industrial application Environmental applications Energy process applications Agricultural applications 9

10. References: https://www.slideshare.net/Saurav Das4/protein-engineering- saurav
https://www.nature.com/subjects/protein-engineering
https://library.um.edu.mo/ebooks/b2805488x.pdf
https://onlinelibrary.wiley.com/doi/book/10.1002/9783527815128 https://www.pdfdrive.com/protein-engineering-and-design- e185690002.html 10