C value paradox unit-ii

Ppt is made vailable for public for scientifc use.
C-value paradox concept in explained by using practical exanples in a simple language

1. C-value paradox; cot curve and
Reassociation Kinetics
By
Mr. K.C.More
Assistant Professor
Department of Botany
SGB Amravati University,
Amravati (MS) India.

2. C VALUES
The amount of DNA in a haploid genome set (such as in a sperm
nucleus/ egg or pollen cell) is called the genome size or C value
where C stands for "constant" or "characteristic" to denote that C
values are relatively constant within a single species, but vary
widely between species

4. EVOLUTION OF GENOME size IN BACTERIA
Nuclear genome:
Chromosomal DNA Plasmid genome:
Plasmid DNA:
Transposable genetic elements

5. Estimation of genomic complexity
Genomic complexity?
How we can measure genomic complexity
of the species with equal genome Size?

24. Cot curves
viruses & bacteria show simple curves
Cot is inversely proportional to genome size

25. Cot curves
eucaryotes show 3 step curves
Step 1 renatures rapidly: “highly repetitive”

26. Cot curves
eucaryotes show 3 step curves
Step 1 renatures rapidly: “highly repetitive”
Step 2 is intermediate: “moderately repetitive”

27. Thank You