Cell proliferation assay services

Cell proliferation assay services

1. Cell Proliferation Assay Services

2. What is Cell Proliferation ? 01

3. Cell proliferation is the process that results in an increase of the number of cells, and is defined by the balance between cell divisions and cell loss through cell death or differentiation.

4. Cell proliferation is increased in tumours .

5. Cells are structural and functional unit of our body which control and maintain the function of all unicellular and multicellular organisms. The process of cell proliferation and differentiation plays a key role from the time of embryogenesis to development of whole organism from single- or double- cell embryo and continues its critical role in maintenance of adult tissue homoeostasis by recycling the old cells with new cells.

6. Phases of the cell cycle Mitosis is the main way for eukaryotes to divide cells. multicellular organisms in a mitotic way to increase the number of somatic cells.

7. Creative Bioarray cell proliferation assay advantages 02

8. Flexible We chose suitable assay method based on the customer’s cell type and requirements. Accurate Sensitive Deliverables Fast The data obtained strongly correlates to the cell number. Capable of detecting low cell numbers. High-throughput, fast turnaround time. Dose response curves and IC 50 values.

9. Different cell proliferation assay technique available 03

10. Detecting proliferation markers Measuring [ATP] Tracking new RNA synthesis Fluorescence-based cell proliferation assays Measures of membrane integrity 3D cell proliferation assays We are capable of performing different cell proliferation assays based on several concepts DNA synthesis cell proliferation assays Metabolic cell proliferation assays Detecting proliferation markers

11. DNA synthesis cell proliferation assays Principle: Proliferating cells incorporate 3H-thymidine/ BrdU labels into their nascent DNA, which can be washed, adhered to filters and then measured. Dye Label: 3H-thymidine, BrdU Feature: Traditional method, reliable Application: This is suitable for immunohistochemistry, immunocytochemistry, in-cell ELISAs, flow cytometry analysis and high-throughput screening.

12. Metabolic cell proliferation assays Principle: Measure of cell proliferation based on the metabolic activity of a population of cells can be used. Tetrazolium salts or Alamar Blue are compounds which become reduced in the environment of metabolically active cells, forming a formazan dye that subsequently changes the color of the media, which is caused by increased activity of the enzyme lactate dehydrogenase during proliferation. The absorption of the media-containing dye solution can be read using a spectrophotometer or microplate reader in low- or high-throughput configurations. Dye Label: MTT, XTT, MTS, WST1, WST8 Feature: Mature method for cell proliferation assays, High-throughput Related Cell Proliferation Kits are available in Creative Bioarray .

13. 3D cell proliferation assays Besides, Creative Bioarray also provides 3D cell proliferation assays by using Soft agar assay with optimized growth and assay conditions based on our experiences with   3D cell culturing. We can upgrade the standard package to include 2D vs 3D comparison.

14. Call us today at 631-626-9181 , or email us at info@creative - bioarray.com for a quotation or if any questions ! Our customer service representatives are available 24hr a day! We thank you for choosing Creative Bioarray service.

15. Thank you ！