CHABTER -6.pptx FAMILY HEALT PLANNING PPT

HEALTH

1. SUBJECT:SIMPLE DIAGNOSIS LECTURE:HUSSEIN ALI NOR Compatibility Testing (cross matching)

2. Experiment in which an unknown (patient) serum and (donor) red cells are tested for the detection of unexpected antibodies which are directed against antigens found on the cells Negative results indicate compatibility Compatibility Testing:

3. Purpose Selection of safest blood components for transfusion With acceptable donor ’ s red cell survival rates Without destruction of recipient ’ s red cells Contin …

4. Compatibility testing Confirms ABO compatibility Detects clinically significant unexpected antibodies Compatibility testing:

5. Major cross match: Test donor cells with recipient ’ s serum to detect antibodies in patient Minor cross match: Test donor serum with recipient ’ s red cells to detect antibodies in donor plasma Types of cross match:

6. Major & minor cross matching: Contin ….

7. Identification of patient & its sample patient full name & hospital registration name of requesting Clinician date & time of sample collection initials of Technician information clearly written on requisition form and sample Steps in Cross-Matching:

8. ABO & Rh of recipient and donor blood Test for clinically significant red cell antibodies on patient serum. Contin …

9. 4. Selection of appropriate unit of blood ABO & Rh compatible Expiration date Contin ….

10. 5.Performance of serological cross match 6.Labeling of component with patient identification details Contin ….

11. 7.Issue after verification of patient identity alongwith compatibility report & reaction form Contin ….

12. Donor Unit Testing ABO grouping Rh grouping: Rh (D) Pre-transfusion testing procedure:

13. Recipient Testing ABO grouping: Forward Rh grouping Cross match Contin …

14. Cross matching should be performed at following phases Saline phase at room temperature AHG phase Cross Matching Procedure:

15. Detects only IgM antibody, reactive at 22 o C. Clinically significant IgG antibody reactive at 37 o C not detected Immediate Spin Technique (IST):

16. Immediate Spin Technique (IST): Contin …..

17. Conventional AHG-Cross match : Conventional AHG-Cross match:

18. Cross matching: Clinical Urgency: Clinical urgency :

19. Selection of alternative blood group for transfusion:

20. Expired / contaminated reagents Improper cell concentration (cell : serum ratio) Labeling errors Improper washing of red cells before forward grouping Equipment – Improper centrifugation Dirty glass wares Poor Documentation Technical Problems: Normal sample Hemolysed

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