Chemical synthesis of dna

PHOSPHORAMADITE METHOD

1. Pt. RAVISHANKAR SHUKLA UNIVERSITY, Raipur S.o.S in Biotechnology TOPIC : - Chemical synthesis of DNA GUIDED BY : Dr. Afaque Quraishi SUBMITTED BY – P . Sujata M.Sc. 3 rd Sem 1

2. Content Introduction : Chemical Synthesis of DNA Gene Machine Oligonucleotide synthesis Evolution of Chemical Synthesis Phosphodiester synthesis Phosphotriester synthesis Phosphite Triester Synthesis Phosphoramidite method : chemical synthesis of DNA steps involved : Detrylation Activation and coupling Capping Oxidation Recovery of final product Assembly of the product Significance of the phosphoramidite method Application Conclusion Reference 2

3. Introduction : Chemical Synthesis of DNA 3

4. Gene Machine OR DNA Synthesizer 4

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6. Old version of DNA Synthesizer 6

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8. Oligonucleotide Synthesis : In General 8

9. Evolution of Chemical Synthesis 9

10. Phosphotriester synthesis 10

11. Phosphite Triester Synthesis 11

12. Phosphoramidite method : chemical synthesis of DNA 12

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14. Structure of phoshoramidite 14

15. Phosphoramidite Method 15

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23. Recovery of the Final Product . 23

24. Assembly of the DNA 24

25. Significance of Phosphoramidite Method 25

26. Applications 26

27. Conclusion 27

28. Reference www.academic.oup.com www.Slideshare.net www.ncbi.nlm.nih.gov Molecular biotechnology : Principles and applications of recombinant DNA :-textbook by Bernard R. Glick and Jack J. Pasternak 4 th edition

29. THANK YOU 29