Colony Morphology and Characteristics of Culture

COLONY MORPHOLOGY AND CHARACTERISTICS OF CULTURE

1. COLONY MORPHOLOGY & CHARACTERISTICS OF CULTURE SUBMITTED TO SUBMITTED BY Dr. ANU.P.A AKHILA K.S ASSISTANT PROFESSOR, SIMI V.K DEPARTMENT OF BIOTECHNOLOGY FIRST YEAR, ST.MARY’S COLLEGE, THRISSUR MVOC. APPLIED BIOTECHNOLOGY ST.MARY’S COLLEGE, THRISSUR

2. COLONY & COLONY MORPHOLOGY A colony is defined as a visible mass of microorganisms, all originating from a single mother. So a colony consist of clone of cells genetically alike. Microorganisms like bacteria grow on solid media as colonies. Colonies are described on the basis of size, shape, texture, elevation, pigmentation and effect on growth medium. So in order to identify a bacteria and fungi we should know how an organism grow in or on media. AKHILA K.S & SIMI V.K,ST.MARY'S COLLEGE,THRISSUR 2

3. The identification of the cultural characteristics of a bacterium on agar plate is called colony morphology . This study of colony morphology can be very useful in distinguishing microorganism from each other. AKHILA K.S & SIMI V.K,ST.MARY'S COLLEGE,THRISSUR 3

4. CHARACTERS COLONY SHAPE FORM ELEVATION MARGIN COLONY SIZE COLONY COLOUR/PIGMENTATION APPEARANCE OF COLONY SURFACE COLONY ODOUR OPACITY CONSISTENCY/TEXTURE AKHILA K.S & SIMI V.K,ST.MARY'S COLLEGE,THRISSUR 4

5. COLONY SHAPE Form of colony It refers to the shape of the colony. The most common shapes are circular, irregular, filamentous, rhizoid, punctiform, spindle etc. AKHILA K.S & SIMI V.K,ST.MARY'S COLLEGE,THRISSUR 5 https://www.ruf.rice.edu/~bioslabs/BIOC318/Image_files/Morphology/form.png

6. Elevation of colony This describes as the side view of a colony. The most common are flat, raised, umbonate, crateiform, convex, pulvinate . AKHILA K.S & SIMI V.K,ST.MARY'S COLLEGE,THRISSUR 6 https:// i1.wp.com/microbeonline.com/wp-content/uploads/2013/09/Elevation-of-bacterial-colony.jpg

7. Margin of colony This refers to how the edge of each colony is seen. Some are entire/smooth, irregular, undulate/wavy, lobate , curled, filamentous etc. AKHILA K.S & SIMI V.K,ST.MARY'S COLLEGE,THRISSUR 7 https://www.ruf.rice.edu/~bioslabs/BIOC318/Image_files/Morphology/margin.png

8. Size of colony The diameter of a colony may be measured in millimeters. described in relative terms such as pinpoint, small, medium, large. AKHILA K.S & SIMI V.K,ST.MARY'S COLLEGE,THRISSUR 8 PINPOINT SMALL MEDIUM LARGE https://www.google.com/url?sa=i&url=https%3A%2F%2Fwww.researchgate.net%2Ffigure%2FColonies-were-classified-into-four-different-size-categories-according-to-their-diameter_fig3_12151058&psig=AOvVaw1JL2Ig3PtxucQJzb2--uhp&ust=1610555928604000&source=images&cd=vfe&ved=0CAIQjRxqFwoTCKC65sHqlu4CFQAAAAAdAAAAABAD

9. Pigmentation of colony Some bacteria produce pigments when they grow in the medium. Some bacteria capable are Staphylococcus aureus , Stretomyces sp., Bacillus sp., Chryseobacterium indologenes , etc. AKHILA K.S & SIMI V.K,ST.MARY'S COLLEGE,THRISSUR 9 https://laboratoryinfo.com/wp-content/uploads/2019/04/Images-of-the-bacterial-colony-with-a-varying-degree-of-pigmentation.jpg

10. Appearance of colony surface Colonies are frequently shiny and smooth in appearance. But some may be dull, rough, wrinkled, glistening etc. AKHILA K.S & SIMI V.K,ST.MARY'S COLLEGE,THRISSUR 10 https:// www.researchgate.net/profile/Haider_Alnaji2/publication/328342721/figure/fig1/AS:682733193940992@1539787686020/Smooth-S-and-rough-R-colonies-of-S-pneumoniae.png https:// www.researchgate.net/profile/Kimberley_Mclaughlin/publication/305121090/figure/fig8/AS:382504377831430@1468207558682/Variation-in-colony-formation-on-agar-plates-during-fitness-assay-KB-plates-inoculated.png https://pbs.twimg.com/media/DY3IcapUMAIlRVD.jpg GLISTENING WRINKLED ROUGH SMOOTH

11. Colony Odour Some microbes produce a characteristics smell which sometimes help in identification. For example actinomycetes produce an earthy odour which is experienced after a rain. Many fungi produce fruity smell E.coli produce a faecal odour. AKHILA K.S & SIMI V.K,ST.MARY'S COLLEGE,THRISSUR 11

12. Opacity of colony The opacity of microbial colony can be: Transparent(clear) Translucent(almost clear with distorted vision) Opaque(not clear) AKHILA K.S & SIMI V.K,ST.MARY'S COLLEGE,THRISSUR 12

13. Opacity of colony AKHILA K.S & SIMI V.K,ST.MARY'S COLLEGE,THRISSUR 13 https://en.wikipedia.org/wiki/File:Streptococcus_pyogenes_(Lancefield_Group_A)_on_Columbia_Horse_Blood_Agar.jpg https://en.wikipedia.org/wiki/File:Staphylococcus_aureus.jpeg TRANSPARENT OPAQUE TRANSLUCENT https://www.google.com/url?sa=i&url=https%3A%2F%2Fmicrobeonline.com%2Fcolony-morphology-bacteria-describe-bacterial-colonies%2F&psig=AOvVaw2JWy9hhKwxB0ZIoa467GtG&ust=1610289127366000&source=images&cd=vfe&ved=0CAIQjRxqFwoTCLD5rZSKj-4CFQAAAAAdAAAAABAK

14. Consistency or texture of colony Some are butyrous (buttery), viscid(sticky), brittle(dry/breakable), mucoid (mucus like). AKHILA K.S & SIMI V.K,ST.MARY'S COLLEGE,THRISSUR 14 https://www.researchgate.net/publication/304614226/figure/fig4/AS:411371100491777@1475089921104/Dry-chalky-white-colonies-of-Nocardia-grown-on-blood-agar.png https://www.google.com/url?sa=i&url=https%3A%2F%2Fwww.researchgate.net%2Ffigure%2FString-test-for-Klebsiella-pneumonia-mucoid-colonies_fig3_319130439&psig=AOvVaw17A9dmL9NSzIisrw1qfJm5&ust=1610285436946000&source=images&cd=vfe&ved=0CAIQjRxqFwoTCMCvte76ju4CFQAAAAAdAAAAABAD https://www.google.com/url?sa=i&url=https%3A%2F%2Fwww.microbiologyinpictures.com%2Fbacteria-photos%2Fpseudomonas-aeruginosa-photos%2Fmucous-pseudomonas-aeruginosa.html&psig=AOvVaw31ajros93ZXrodiJjdACV_&ust=1610285510732000&source=images&cd=vfe&ved=0CAIQjRxqFwoTCKib_JH7ju4CFQAAAAAdAAAAABAD BRITTLE STICKY MUCOID https:// www.google.com/url?sa=i&url=https%3A%2F%2Fwww.morton-pub.com%2Fsites%2Fdefault%2Ffiles%2Fpromos%2Fcustomlab_cover_wpages_micro.pdf&psig=AOvVaw0Cs67KNRbyhJFpLGpomthR&ust=1610290070589000&source=images&cd=vfe&ved=0CAIQjRxqFwoTCKCZnpWMj-4CFQAAAAAdAAAAABAK BUTYROUS

15. Characters in a glance AKHILA K.S & SIMI V.K,ST.MARY'S COLLEGE,THRISSUR 15 https://player.slideplayer.com/78/12828098/slides/slide_12.jpg

16. AKHILA K.S & SIMI V.K,ST.MARY'S COLLEGE,THRISSUR 16 Circular, entire margin, opaque, smooth, golden yellow Filamentous, filiform, opaque, wrinkled, white, convex Circular, entire, opaque, umbonate, smooth, red Rhizoid, filiform, opaque, raised, rough, white Circular, entire, opaque, flat, grey, smooth Irregular, undulate margin, opaque, pulvinate, rough Irregular, undulate, opaque, weak umbonate, white smooth Circular, entire margin, opaque, smooth, black, pulvinate SOME BACTERIAL COLONIES

17. REFERENCES Lansing.M.Prescott, Harley & Klein, Microbiology 5 th edition,(pg.no.-108-110). ISBN-0-07-282905-2 https://slideplayer.com/slide/14572325/ https://www2.slideshare.net/vivekaiden/colony-morphology-characteristics-of-cultures https://microbiologysociety.org/why-microbiology-matters/what-is-microbiology/bacteria/observing-bacteria-in-a-petri-dish.html 17 AKHILA K.S & SIMI V.K,ST.MARY'S COLLEGE,THRISSUR

18. 18 AKHILA K.S & SIMI V.K,ST.MARY'S COLLEGE,THRISSUR T H A N K Y O U