Epidemiological triad [1] 1.The agent is the harmful etiological factor that causes health problems. The etiological factor (agent) may cause disease either by its presence or by its absence. The causative agent may be a substance, living or non-living, excessively present or lacking, tangible or non-tangible. 2. Physical Agents These include mechanical forces such as friction, extreme heat, cold, humidity, pressure, sound, radiation, electricity, etc. 3. Biological Agents Living organisms, such as bacteria, fungi, chlamydia, rickettsiae, protozoa, viruses, mycoplasma, helminths. 4. Chemical Agents Endogenous chemical agents are those which are made by the body as a result of abnormal metabolism, for example urea (uremia), serum bilirubin (jaundice), ketones (ketosis, keto- acidosis), uric acid (gout), calcium carbonate(renal stones), etc .