CURCUMIN (Curcuma longa)

Isolation, identification and analysis of CURCUMIN

1. BY G.JYOTHI SIRISHA 178N1R0028

2. OUTLINE Synonym Biological source Chemical constituents Isolation Identification Analysis Uses

3. SYNONYM: Indian Saffron, Turmeric, Haldi, Haridra BIOLOGICAL SOURCE: Turmeric cosists of dried as well as fresh “Rhizomes” Of the plant Curcuma longa FAMILY: Zingiberaceae COLOUR: Orange yellow to yellow ODOUR: Aromatic TASTE: Warmly aromatic & Bitter ORGANOLEPTIC CHARACTERS

4. PLANT HABITAT Turmeric is a perennial herbaceous plant The leaves are alternate and arranged in two rows CHEMICAL CONSTITUENTS Major constituents: Curcumin 50-60% Essential oil 2-7% with high content bisabolane derivatives It also contains Desmethoxycurcumin(DMC), Bisdesmethoxycurcumin(BDMC), common phytosterols, ar-tumerone, Zingiberene fatty acids and polysaccharides.

5. ISOLATION OF CURCUMIN Curcumin can be obtained by different process Turmeric powder is extracted with alcohol in soxhlet extractor The alcoholic extract is concentrated under reduced pressure and dried

6. 2. In another procedure, turmeric powder is first extracted with hexane followed by Acetone The acetone extract is concentrated with hexane followed by acetone The acetone extract is concentrated & dried to yield CURCUMIN

7. IDENTIFICATION TESTS Chemical Test: Powered drug + sulphuric acid gives Crimson colour The aqueous solution of turmeric + boric acid gives reddish brown colour which on addition of alkali changes to greenish blue TLC method Sample preparation : Dissolve 1mg of curcumin in 1mL of methanol Stationary phase : Silica gel G Mobile phase : Chloroform:Ethanol:Glacial acetic acid (94:5:1) Detecting Agent : Anisaldehyde sulphuric acid reagent Rf valve : 0.79,0.60 and 0.43 Colour Spot : Bright yellow fluorescent

8. ANALYSIS OF CURCUMIN METHODS : By spectrophotometeric Method Estimation by HPLC Estimation by Alkalimetric Method ESTIMATION BY HPLC Column : 5um Nucleosil NH2 (Chromapack) Mobile Phase : Etahnol Flow rate : 1.2mL/min Detection : UV-254nm Peak retention time: Bisdemethoxy curcumin 4.5-5 minutes Demethoxy curcumin 6-7 minutes Curcumin 11-12 minutes

9. USES OF CURCUMIN

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