Cyclophosphamide

CYCLOPHOSPHAMIDE (Chemotherapy Drug)

1. CYCLOPHOSPHAMIDE Ferdinand F. Fatiga , RMT

2. CYCLOPHOSPHAMIDE Used in the treatment of WBC cancers (AML/ALL) and Lymphoma Cell Cycle – Non specific Alkylating agent Adds Alkyl group (C n H 2n+1 ) to DNA

3. CYCLOPHOSPHAMIDE Mechanism of Action the main effect of cyclophosphamide is due to its metabolite P hosphoramide mustard Phosphoramide mustard forms DNA crosslinks between ( interstrand crosslinkages ) and within ( intrastrand crosslinkages ) DNA strands at guanine N-7 position, this eventually leads to cell death

4. CYCLOPHOSPHAMIDE Activation

5. CYCLOPHOSPHAMIDE

6. CONCLUSION Expression of genes that code for Cytochrome P450 (Oxidase Enzyme) and ALDH enzymes affects the sensitivity of Cancer cells to Cyclophosphamide since these enzymes affect the metabolism of the prodrug (Cyclophosphamide) to its active Phosphoramide mustard metabolite.