Diapositivas Seminario Biomol Mauricio Berbesi y Simón Berrio.pdf

Seminario biología molecular Simón Berrio y Mauricio Berbesi

1. Mauricio BerbesiZaccagnini
Simón Berrio Domínguez
Tercer Semestre
Universidad Pontificia Bolivariana

2. Triple Negative Breast
Cancer
INTRODUCTION
Metastases Shikonina

3. INTRODUCTION

4. GENERAL OBJETIVE

5. Determine native protein
massesand identify
physiologicalprotein-
proteininteractions
Blue-Native
Polyacrylamidegel
Electrophoresis(PAGE)
METHODS

6. METHODS
Western Blottingand
Immunoprecippitation
Assay
Seehowinvolved
metastasesisin the
processofcell
signaling

7. METHODS
Immunofluorescence
staining
Utilizesa pairing
betweenantigenand
antibodytoidentifythe
depositionofabnormal
proteinsin thetissue

8. METHODS
Q-PCR
Itisa test thatdetects
DNA and RNA froma
pathogenorabnormal
cells

9. EpCAM: Adhesion transmembrane
protein
CAF: Cancer-associated fibroblast
Results
C-D: Result shows how CAF
can grow uncontrolled, but
with shikoninpresence, CAF
stop its growth development
F:Detection of EpCAM
protein in red, with shikonin
you can see less presence

10. D: Detection of mitochondrial
complexes, there is greater
detection of 3
rd
mitochondrial
complex
E: In red, you can see
mitocondrial distribution. With
shikonin, mitochondria
decrease.
Results

11. B:Expression of PGC-1α
and ERRα in different
breast cancer cell types
D: Cytoplasmatic an
nuclear distributions of
PGC-1α and ERRα in TNBC
cells.
J: Protein Levels in
Mitochondria and total
cells.
CAF and Shikonin
presencecomparison.
Results

12. Shikoninincreased the
interaction of PGC-1α with
other proteins to avoid its
action. CAF and Shikonin
presence comparison
Results

13. DISCUSSION

14. CONCLUSION
Inhibit some proteins
in CAF, can stop
metastases
Shikonin proved being
efective inhibiting
mitocondrial biogenesis
Recognize the effect
of shikonin in TNBC

15. —Someone Famous
This is a quote. Words full of
wisdom that someone important
said and can make the reader get
inspired.”
“

16. Mauricio Berbesi

17. Simón Berrio