dna fingerprinting.pdf

chemstry project work

1. JAWAHAR NAVODAYA VIDYALAYA
FATEHPUR


SESSION – 2022-23
BIOLOGY PROJECT WORK

SUBMITTED BY --- SUBMITTED TO ----
SUGHRA FATMA MRS.KALPNA SAGAR
ROLL NO. ___________ (P.G.T. BIOLOGY)
CLASS XII

2. BIODATA

NAME – SUGHRA FATMA
FATHER’S NAME – MR. SHAFIQ ALI
MOTHER’S NAME – MRS.RAZIA JAMAL
D.O.B. – 04/07/2006
SCHOOL – JAWAHAE NAVODAYA VIDYALAYA
FATEHPUR(U.P)
ROLL NO. - ________________
SESSION – 2022-23

3. CERTIFICATE

This is to certify that SUGHRA FATMA
“XIIth Science” has completed her project in
biology for academic year 2022-23 as per the
term and condition of CBSE syllabus. She has
perform the entire practical and project under
the guidance of Mrs. Kalpna Sagar (P.G.T
Biology)

Certified By-__________________

4. AIM

DNA
FINGERPRINTING

5. INDEX
SR. CONTENTS
1.
ACKNOWLEDGEMENT
2.
INTRODUCTION
3.
HISTORY OF DNA FINGERPRINTING
4. WHAT IS DNA FINGERPRINTING
5. DNA FINGERPRINTING TECHNOLOGY
6. BIOLOGICAL MATERIAL USED FOR
DNA FINGERPRINTING
7. STAGE OF DNA FINGERPRINTING
8. DNA fingerprinting: Methods
9. DNA FINGERPRINTING :
APPLICATIONS
10. CONCLUSION

6. ACKNOWLEDGEMENT

I AM THANKFUL TO MRS. KALPNA SAGAR
WHO PROVIDE ME AN INTRESTING PROJECT.
I AM INDEBTED TO FACULITY BY J.N.V.
FATEHPUR FOR IT’S CONTINUOS
SUPERVISION AND GUIDANCE DURING THE
PREPARATION OF MY PROJECT.
I AM ALSO THANKFUL TO MY FRIEND’S WHO
HAVE CO-OPERATED IN THE COMPLETION OF
THIS PROJECT.

IN THE LAST BUT NOT LEAST.

I AM THANKFUL TO MRS.NEETA UPADHYAY,
PRINCIPAL OF J.N.V. FATEHPUR FOR
PROVIDING ME ON REQUIRED FACILITIES
DURING THE PREPARATION OF MY PROJECT.

7. INTRODUCTION

The process of DNA fingerprinting was invented
by Alec Jeffreys at the University of Leicester
in 1985. He was knighted in 1994.
DNA fingerprinting or DNA profiling, any of
several similar techniques for analyzing and
comparing DNA from separate sources, used
especially in law enforcement to identify
suspects from hair, blood, semen, or other
biological materials found at the scene of a
violent crime. It depends on the fact that no two
people, save identical twins, have exactly the
same DNA sequence, and that although only
limited segments of a person's DNA are
scrutinized in the procedure, those segments
will be statistically unique.

8. HISTORY OF DNA FINGERPRINTING

Up to 1948, the only method of establishing and
authenticating personal identification was by
the fingerprint process.

DNA fingerprinting technique was devised in
1985 by Alec Jeffrey at University of Leicester
in England, while working on the sequences
within myoglobin gene.

9. WHAT IS DNA FINGERPRINTING

It is a technique, by which an individual can be
identified at molecular level. With the
advancement of science and technology STR
analysis has become very popular in forensic
laboratories. Scientists have chosen repeating
sequences in the DNA, which are present in all
individuals on different chromosomes, and are
known to vary from individual to individual
except in identical twins. These are used as
genetic markers to identify the Individual.

10. DNA FINGERPRINTING TECHNOLOGY

DNA fingerprinting technology has made it
possible to identify the source of biological
samples found at scenes of crime. This will
resolve disputes of maternity/paternity,
identification of mutilated remains,
identification of rape/murder, identification of
missing child , exchange of babies in hospital
wards, forensic wildlife, protection of farmers
right and biodiversity. This remarkable
technology provides exclusion as well as
positive identification with virtually 100%
precision.

11. BIOLOGICAL MATERIAL USED FOR
DNA FINGERPRINTING

Blood
Hair
Saliva
Semen
Body tissue cells

DNA samples have been obtained from
vaginal cells transferred to the outside of a
condom during sexual intercourse

12. STAGE OF DNA FINGERPRINTING
Cells broken down to release DNA

DNA strands cut into fragments
Fragment separated
Pattern of fragment analysed

13. DNA fingerprinting: Methods

A common procedure for DNA fingerprinting is
restriction fragment length polymorphism (RFLP). In
this method, DNA is extracted from a sample and
cut into segments using special restriction enzymes.
RFLP focuses on contain sequences of repeated DNA
bases, which vary widely from person to
person. The segments are separated using a
laboratory technique called electrophoresis, which
sorts the fragments by length. The segments are
radioactively tagged to produce a visual pattern
known as an
autoradiograph, or DNA fingerprint, on X-
ray film. A newer method known as short
tandem repeats (STR) analyzes DNA segments for
the number of repeats at 13 specific DNA sites.
The chance of misidentification in this procedure is
one in several billion. Yet another
process, polymerase chain reaction, is used to
produce multiple copies of segments from a very
limited amount of DNA (as little as 50 molecules),
enabling a DNA fingerprint to be made from a single

14. hair. Once a sufficient sample has been produced, the
pattern of the alleles (see genetics) from a limited
number of genes is compared with the pattern from the
reference sample. A non match is conclusive, but the
technique provides less certainty when a match occurs

15. DNA FINGERPRINTING : APPLICATIONS


DNA fingerprinting can be applied in the following
scenarios:
Establishment of paternity and Maternity
Establishment of the parentage for
child swapping cases
Establish the identity of the rapist in rape cases
Identification of mutilated remains In murder,
bomb blast, air crashes etc.
Wild life identification, and Seed authentication.

16. CONCLUSION

In conclusion, while evidences can
be tampered with and witnesses can
turn hostile, DNA will never lie. It will
indeed, unravel the truth even
several decades after a crime has
been committed. Such are the
powers of DNA Fingerprinting.