Dna microinjection

DNA Microinjection Procedure

1. DNA MICROINJECTION

2. INTRODUCTION DNA Microinjection is one of the method used for production of transgenic animals. Organism containing integrated sequences of cloned DNA, transferred using techniques of genetic engineering are called transgenic animals. This method allows an early integration of the transgene into the host DNA, which is important to ensure that transgenic DNA is apparent in all of the host.

3. PROCEDURE

4. ADVANTAGES This technique is simple. It is applicable to wide range of species. Most widely used method.

5. DISADVANTAGES Time consuming procedure. Low success rate. Unpredictable effects due to random transgenic integration-gene is over expressed or under expressed. Costly

6. References: Liu, C., Xie, W., Gui, C. & Du, Y. Pronuclear microinjection and oviduct transfer procedures for transgenic mouse production. Methods Mol. Biol. 1027 , 217–232 (2013). Singhal, M. & Kansara, N. Transgenic Animals: Production and Application. Pharm. Sci. 1 , 12–22 (2010).

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