DNA replication in bacteria

All the process in DNA replication in bacteria .

1. MEWAR UNIVERSITY PRESENTATION BY:- rambhagat mandal Bsc.Ag 1 st year MUR 1500210 SUBMITTED TO :- Mr. SAURABH SAXENA PRESENTATION TOPIC :- DNA REPLICATION

2. DNA REPLICATION IN BACTERIA CELL DNA replication is complete in three steps :- 1.INITIATION 2.ELONGATION 3.TERMINATION * Semiconservative :-When replication is starts then double stranded DNA become half of parential molecule and half of new molecule is known as semiconservatie .

3. 1.INITIATION There are many enzymes involve. 1.Primase:-Makes RNA primers from DNA template . 2 .DNA polymerase:-Synthesis of DNA 3 .Topoisomerase :-Relaxes super coiling ahead of replication fork. 4 .Helicase :-unwind and seprate d.s.DNA . 5 .DNA ligase :-Makes covalent bond to join okazaki fragments and repair it.

4. 7 .Single strand binding proteins:-Synthesis of new strand. 8 .Transposase :-Cuts sugar of DNA leaving single strand’sticky ends’.

5. 2.ELONGATION Synthesis of DNA in each old strand which synthesis of new complementry strand.There are many proteins are invoved in protein synthesis . DNA polymerase 3 remove mismach nucleotide and put correct nucleotide.

6. 3.TERMINATION After 40 minutes two replication fork meet,two inter wined DNA molecules one seperated by other enzymes sugaranteeing that each daughter cell will inherit one complete chromosome after binary fission. A)LEADING STRAND SYNTHESIS:- DNA polymerase read the template in the 3’-5’ direction , bringging in triphosphate nucleotide that hydrogen bond with their coplement in the template strand.

7. Triphosphate nucleotides provide the DNA polymerase to covalently join nucleotides into the continuous strand forming and elongating chain of nucleotides from 5’-3’. B)LAGGING STRAND SYNTHESIS:- DNA polymerase moves away from the replication fork and discontiuous process starts and stop occurs with the new strand always lagging behind the leading strand is called as lagging strand ( okazaki fragments). DNA ligase join okazki fragments into a complete and elongating single strand.

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