Dried human plasma, thrombin and fibrinogen

Short Note on dried human plasma, thrombin and fibrinogen by Javed

1. C l a s s s e m i n a r O n D r i e d h u m a n p l a s m a T h r o m b i n Fibrinogen P r e s e n t e d by J a v e d B . p h a r m a 3 r d y e a r

2. D r i e d h u m a n p l a s m a I t c o n sist a b o u t 5 5 % o f b l o o d . T h i s i s y e l l o w c o l our f l u i d . I t i s u s e d f o r p l a s m a transfusion w h e r e m e d i c a l l y n e e d ed . I t w a s f i r s t d e v e loped i n British 1 9 3 . C o l l e c tion T a k e b l o o d f r o m b l o o d d o n or w h i c h w a n t t o d o n ate b l o o d a n d e l i g ible f o r d o n a t i ng b l o o d . S e p a r ate t h e b l o o d p l a s m a f r o m b l o o d b y t h e p r o c ess o f c e n t r i f u g a t ion

3. P r o c e d ure P r o c e d u re T a k e 4 ml bloo d p l a s m a w h i c h a r e s e p a rated f r o m b l o o d . Packed i n t o M R C b o t t l e ( m e d i c a l r e s e arch council b o t t le F r e e z i ng - T h e b o t t l e i s s e a l ed w i t h B a c t e r i o l ogical e f f i cient . T h e bottle i s t h e n centrifuged a t 1 8 ° c

4. P r i m ary d r y i ng - T h e b o t t l e a r e mounted h o r i zontally i n the d r y i ng c h amber a t 5 ° c T h i s p r o c e s s take s p l a c e a b o u t 2 w e e k s Second ary d r y i n g - T h i s i s d o n e i n a n o t her c h a m ber b y v a c c u m e d e s i c c a t ion o v e r p h o s p h o rus p e n t a o x i d e I t t a k e s p l a c e a b o ut a d a y A t l e a st . 5 % m o i s t u r e s h o u ld b e l e f t

5. S t o r a g e - I t i s k e p t b e l ow 2 8 ° c a n d p r o t ect ed f r o m m o i s t u re , s u n l i g ht a n d r e m a i ns u s a b l e f o r 5 y e a r 8 U s e s - B l o o d clotting A utoimmune disorders hemophilia O ther medical emergencies

6. T h r o m bin & fibrinogen P r e p aration T a k e c i t r a t e d p l a s m a ( n o t m o r e t h a n . 4 % ) A d d o r g a n ic s o l v e nt h a v i n g p r o p e r v o l a t ility & b a c t e r i o s tatic a c t i vity , A stabilizer s u c h a s s o d i u m c a p r y l a t e . W h o s e f u n c tion i s t o a l l ow t h e p r e p aration t o b e h e a t e d f o r one h o u r a t l o w t e m perature w i t h o ut d e n a t u r a t i on o f p r o t ien . F o r i s o t o n i c i t y N a c l a d d e d a n d t o c o n t r o l t h e c o n t a m ination B o t h proteins a r e p r e s ent i n b l o o d p l a s m a t h e s e pro t eins a l s o u s e d i n b l o o d c l o t ting .

7. M e c h anism o f action P r o t h r o m bin T h r o m bin I n a c tive I n s o l u ble A c t i ve S o l u b le Fibrinogen Protein S F i b r i n ( m o n o m er F i b r o us i n nature I n s o l u b le ) F i b r i n P o l y m ers Haemosta t i s B l o o d c l o t ting

8. Thank y o u