Enhanced biosynthesis of hydrogen using nanoscale iron oxide-complex.pptx

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1. Enhanced biosynthesis of hydrogen using nanoscale organic iron oxide-complex particles for sustainable energy solution Presented by: FARIHA KANWAL

2. Study Background The significant development of industrial economy, transformation in living style and increasing number of vehicles on roads has raised global energy demand. In the current scenario, petroleum based fuel is fulfilling more than 80% of prime energy requirements worldwide, while 60% of its share is consumed by transportation division( Joshi, Pandey, Rana, & Rawat , 2017 ). The growing populations have more energy demands and resulting in the generations of deleterious pollutants in the atmosphere. Hence, the today’s most important issues are the global warming and climate change in global policy and science ( Lorenzi , 2012 ). It is reported by the International Energy Agency (IEA) that the release of carbon dioxide gas from energy sector will rise from 50% in 2030 to 80% in 2050. Thus, the environmental issues related to the fossil fuels utilization are the major concern for the international community ( da Silva Veras et al ., 2017 ) .

3. International Agreements 1 2 3 The Rio de Genaro Earth summit 1992 was the first attempt followed by Kyoto Protocol 1997. In Doha Amendment of Kyoto Protocol 2012, states united their motivations to reduce the level of greenhouse gases in the atmosphere and expected an improvement towards the sustainable future by launching the technology and financial support for the developing nations ( Oncel , 2017 ). Paris Agreement passed in November 2016 force the world to minimise the global warming to less than 2◦C compared to levels of pre-industrial era by declining the use of fossil fuels and moving towards a low carbon economy. According to some researchers , this is the last turn before the wall ( Namsaraev , Gotovtsev , Komova , & Vasilov , 2018 ) .

4. Sustainable Hydrogen Economy In the modern era, biohydrogen has become a promising biofuel as it is clean and efficient energy carrier. Hydrogen has many benefits since it has the highest energy per unit mass (142 Kjg -1 ). Bio hydrogen can be produced by thermo-chemical and biological ways. The biological technologies for hydrogen production are preferred due to their ecological benefits. These biological processes are also less energy intensive and more environmental friendly in terms of global reduction of carbon dioxide. The renewable hydrogen production techniques has potential to become cost effective because they can use less expensive raw biomass as feedstock e.g; agricultural, municipal and industrial wastewater and organic waste ( Bundhoo & Mohee, 2016 ; Ghimire et al ., 2015 ).

5. Photo-fermentation

6. Objectives of the Study 1 2 3 Development of cost effective methodology for sustainable energy solution . Bio-fabrication of nanoscale organic iron oxide complex Enhanced biosynthesis of hydrogen using organic iron oxide complex 3 4 Utilization of organic industrial wastewater for the production of biohydrogen.

7. Experimental Phase 1

8. Experimental Phase 1 Collection of water samples Bacterial Enrichment Bacterial colonies Strain purification Gram-staining Isolation and purification of bacterial cultures

9. Experimental Phase 2 Fabrication of organic iron oxide complex . Collection of Eucalyptus leaves Washing of leaves Drying Grinding Boiling in D.I water Vacuum filtration Leaf extract 0.1 M FeSO4.7H2O + Freeze drying Centrifuge Preparation of nano- sclae black particles Black powder of organic iron oxide complex

10. Experimental Phase 2 Pectoral Description

11. Experimental Phase 2 Characterization of organic iron oxide complex Characterization UV Vis FTIR XRD SEM TEM

12. Experimental Phase 3 Biohydrogen Production

13. Experimental Phase 3 Chemicals for bacterial media preparation Basal Media (pH 6.8-7.2) Sr No. Components g L -1 1 KH 2 PO 4 0.33 2 NaCl 0.33 3 MgSO 4 0.33 4 CaCl 2 .2H 2 O 0.05 5 NH 4 Cl 0.5 6 Sodium succinate 1.0 7 Yeast Extract 1.0 8 Agar 17 Trace Salt Solution (pH 3-4) 9 ZnSO 4 .7H 2 O 0.01 10 MnSO 4 .7H 2 O 0.003 11 H 3 BO 3 0.03 12 CoCl 2 .6H 2 O 0.02 13 CuCl 2 .2H 2 O 0.001 14 NiCl 2 .6H 2 O 0.002 15 Na 2 MoO 4 0.003 16 Cysteine - Hcl 0.5

14. RESULTS Macrogen Inc. (Seoul, South Korea )------ didoxy sequencing of 16S rRNA gene Phylogenetic tree for SP6

15. Phylogenetic tree for MP2

16. Phylogenetic tree for MP3

17. Phylogenetic tree for MP4

18. Sr. No. Strain Control 1g 0.8g 0.6g 1 SP6   +++   ++   +   + 2 MP2   +++   ++   ++   + 3 MP3   +++   +++   ++   + 4 MP4   +++   ++   ++   + Effect of yeast extract on bacterial growth without carbon source Gram negative rods

19. Sr . No. Strain Propionate Citrate Acetate Oxalate Lactate 1 SP6 + - LG LG LG 2 MP2 + - + LG LG 3 MP3 ++ - ++ LG LG 4 MP4 + - LG LG LG Response of bacterial strains to different carbon sources

20. FTIR spectra of nanoscale organic iron oxide nanoparticles using Eucalyptus leaf extract

21. XRD pattern of nanoscale organic iron oxide nanoparticles using Eucalyptus leaf extract UV Vis spectra of nanoscale organic iron oxide nanoparticles using Eucalyptus leaf extract

22. SEM image of nanoscale organic iron oxide nanoparticles fabricated using ferrous sulphate and Eucalyptus leaf extract in 1:2

23. TEM image of nanoscale organic iron oxide nanoparticles fabricated using ferrous sulphate and Eucalyptus leaf extract in 1:2

24. EDS and Elemental mapping of nanoscale organic iron oxide nanoparticles fabricated using ferrous sulphate and Eucalyptus leaf extract in 2:1

25. Production of hydrogen by different strains at different concentrations of FeSO4.7H2O

26. Photo-pigment spectra of all the strains at different concentrations of FeSO4.7H2O

27. Optical Density of hydrogen producing strains at different concentrations of FeSO4.H2O       Sr. No.       Strain   O.D at 600nm Control 2.5 mg/L Fe +2 5 mg/L Fe +2 10 mg/L Fe +2 1 MP2 1.36 1.26 1.71 1.37 2 MP3 1.73 1.93 1.94 1.81 3 MP4 1.46 1.37 1.90 1.72 4 SP6 1.38 1.70 1.50 1.80

28. Dry cell weight and total carotenoid content of strains at different concentrations of FeSO4.7H2O       Sr. No.       Strain   Dry Cell weight (g/L) Control 2.5 mg/L Fe +2 5 mg/L Fe +2 10 mg/L Fe +2 1 MP2 0.2 0.4 0.2 0.3 2 MP3 0.3 0.6 0.4 0.6 3 MP4 0.6 0.4 0.5 0.4 4 SP6 0.3 0.4 0.4 0.5       Sr. No.       Strain   Total Carotenoid (mg/g) Control 2.5 mg/L Fe +2 5 mg/L Fe +2 10 mg/L Fe +2 1 MP2 0.023 0.014 0.031 0.017 2 MP3 0.025 0.012 0.023 0.012 3 MP4 0.012 0.014 0.012 0.015 4 SP6 0.019 0.019 0.015 0.011

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