Estimation of MgSO4 &calcium gluconate

Estimation of MgSO4 &calcium gluconate by complexometry

1. ESTIMATION OF MAGNESIUM SULPHATE & CALCIUM GLUCONATE Renjitha J R Assistant Professor Mount Zion College of Pharmaceutical Sciences &Research, Adoor

2. Preparation of 0.05 M Disodium Edetate Dissolve 37.2g of disodium edetate in sufficient water to produce 1000ml. Standardization of 0.05 M Disodium edetate Pipette out 10 ml of 0.05 M CaCl 2 solution into a conical flask & 5 ml of Ph 10 buffer solution. Add 2- 3 drops of solochrome black T indicator& titrate with EDTA solution. Repeat titration for concordant value.

3. 1) Estimation of Magnesium Sulphate Weigh accurately 0.3 g of anhydrous magnesium sulphate , dissolve in 50 ml water. Add 10 ml of ph 10 ammonia buffer & 50 mg mixture of mordant black II & NaCl as indicator. Titrate with 0.05 M disodium edetate until the solution becomes blue. Each ml of 0.05 M Disodium edetate until the solution becomes blue. Each ml of 0.05 M Disodium edetate = 0.012325g of MgSO4. Percentage purity of MgSO4= Volume of EDTA x factor x 100 x Molarity of EDTA(actual) Weight of MgSO4 in gm x molarity of EDTA (expected)

4. 2 ) ESTIMATION OF CALCIUM GLUCONATE INJECTION Calcium gluconate injection contains a quantity of calcium equivalent to not less than 8.5% & not more than 9.4% of stated amount of calcium gluconate . Assay of calcium gluconate is performed by substitution reaction/replacement titration.

5. Procedure To an accurately measured volume equivalent to 0.5 g of calcium gluconate , add 50 ml of water & add 5 ml of 0.05 M MgSO 4 & 10 ml of strong ammonia solution & titrate with 0.05 M disodium edetate using mordant black II mixture as indicator. From the volume of 0.05 M disodium edetate required subtract the volume of the MgSO4 solution added. Each ml of 0.05 m Disodium edetate = 0.02242g Calcium. gluconate